NODETranscriptomicsJiayin PengRNA-Seqhttps://www.biosino.org/node/experiment/detail/OEX00021151Illumina HiSeq 1500Pairedsize fractionationN1['skeletal Muscle']['Mus musculus']For Gli1+MuSCs, a total of 8,508 tdT+ (Gli1+) cells were sorted from hindlimb skeletal muscles of 3 adult male mice and pooled for scRNA-seq. For WT MuSCs, CD45-CD31-Sca1-Vcam1+ MuSCs were isolated from wild-type mice by fluorescence-activated cell sorting (FACS),and analyzed by scRNA-seq.NODEfalseOEX_Jiayin_2212211338We performed scRNA-seq using Gli1-CreERT2; R26-tdTomato mice, in which Gli1+ cells were labeled by tdTomato (tdT) after tamoxifen (TAM) induction. CD45-CD31-Sca1-Vcam1+ MuSCs were isolated from wild-type mice by fluorescence-activated cell sorting (FACS),and analyzed by scRNA-seq.2023-08-012022-12-21OEX00021151OEP00003799