{"database":"NODE","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"submitter":["Delong Feng"],"technology_type":["ChIP-Seq"],"full_dataset_link":["https://www.biosino.org/node/experiment/detail/OEX00024597"],"experiment_platform":["Illumina NovaSeq 6000"],"experiment_library_layout":["Paired"],"experiment_library_selection":["ChIP"],"experiment_mate_pair":["N"],"sample_count":["15"],"tissue":["['pbmc']"],"taxonomy":["['Homo sapiens']"],"experiment_protocol":["ChIP-seq was performed as previously described. Briefly, 2-5 million CD4+ T cells were lysed in cell lysis buffer for 5 min on ice. Cell were pelleted and resuspened with 200 μl of  digestion buffer. Digestion was then performed by adding micrococcal nuclease and incubation for 5 min at 37 °C. The reaction was stopped by EDTA-EGTA buffer . After centrifuge for 15 min at 13000rpm, 1/10 of the supernatant  were taken as Input sample, others supernatant was dilute into a CHIP-IP buffer. Chromatin was then incubated overnight at 4 °C with anti-trimethylated H3K4 antibody . Protein G magnetic beads were added for an additional 4 h of incubation. Immunoprecipitates were washed vigorously and DNA was purified with PCR purification kit. 1ng-50ng H3K4me3 ChIP'ed DNA or input DNA was used for ChIP-seq library construction via TruePrep DNA Library Prep Kit V2 for Illumina. After PCR step, the DNA libraries were purified and size selection by 0.5× and 0.25× DNA beads. ChIP-seq libraries were sequenced via paired-end 150 bp reads on an Illumina platform."],"repository":["NODE"],"additional_accession":[]},"is_claimable":false,"name":"H3K4me3 ChIP-seq for of CD4+T cells form SLE patient and healthy control","description":"H3K4me3 ChIP-seq for of CD4+T cells form SLE patient and healthy control","dates":{"publication":"2024-03-29","submission":"2023-08-12"},"accession":"OEX00024597","cross_references":{"NODE":["OEP00004403"]}}