{"database":"NODE","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Siyu Jing"],"experiment_platform":["ACQUITY QDa"],"full_dataset_link":["https://www.biosino.org/node/experiment/detail/OEX00025210"],"sample_count":["1"],"tissue":["['liver']"],"experiment_protocol":["The resulting spectra from each fraction were searched separately against the homo_sapiens_uniprot_2021_3_9.fasta (194557 sequences) database by the search engine Proteome Discoverer 2.2 (PD 2.2, Thermo). The results of the search and identification by PD2.2 software were imported into Spectronaut (version 14.0, Biognosys) software to generate a library. The eligible peptides and product ions were selected from the spectrum by setting peptides and ion pair selection rules to generate a target list[61]. The DIA data were imported, and ion-pair chromatographic peaks were extracted according to the Target List. The ions were matched, and the peak areas were calculated to qualitatively and quantitatively analyze the peptides. iRT was added to the sample to correct the retention time, and the precursor ion Q value cutoff was set to 0.01. The quantitative values were visualized with Bionic Visualizations Proteomaps (https://proteomaps.net/). Differentially expressed proteins (DEPs; tumor vs. adjacent nontumor, p<0.05, |logFC|>1) underlying enrichment analyses. Gene Ontology and KEGG pathways with adjusted p<0.05 (Benjamini‒Hochberg method) were considered significantly enriched."],"taxonomy":["['Homo sapiens']"],"repository":["NODE"],"additional_accession":[]},"is_claimable":false,"name":"OEX_Siyu_2310032139","description":"OEX_Siyu_2310032139","dates":{"publication":"2023-10-03","submission":"2023-10-03"},"accession":"OEX00025210","cross_references":{"NODE":["OEP00004595"]}}