NODETranscriptomicsYuanliang ZhangRNA-Seqhttps://www.biosino.org/node/experiment/detail/OEX00027260Illumina NovaSeq 6000PairedPCR1['bone Marrow, Liver']['Mus musculus']For bulk RNA-seq, total RNA samples were extracted, and ribosomal RNA (rRNA) was removed using the Ribo-Zero rRNA Removal Kit (MRZMB126, Illumina). Subsequently, rRNA-depleted samples were fragmented, then subjected to first and second strand cDNA synthetization. cDNAs were ligated with sequencing adapters according to standard Novaseq 6000 platform (Illumina) manuscripts.NODEfalseOld_RNA-seqcells from WT and Setd2 deficiency mice were sorted for RNA sequencing.2024-02-272024-01-26OEX00027260OEP00004811