NODEOtherLi HuangAMPLICONhttps://www.biosino.org/node/experiment/detail/OEX00029343Illumina MiSeqPCRPaired12['marine metagenome']RNA and DNA in microorganisms collected on the filters were extracted with the RNeasy® PowerSoil® Total RNA Kit and the RNeasy® PowerSoil® DNA Elution Kit (Qiagen, Germany) according to the manufacturer's protocols. The concentration and quality of final DNA preparations were determined by NanoDrop 2000 UV-vis spectrophotometer (Thermo Scientific, Wilmington, DE, USA). DNA samples were amplified with the barcoded primers 515Fand 907R targeting the V4–V5 hypervariable regions of the 16s rRNA gene. RNAs were also amplified with the same primers after reverse transcription into cDNA with the Promega GoScript™Reverse Transcription System (Promega, USA). PCR reactions and thermocycler programe followed Liu et al. (2020). Each PCR reaction (50 µl), carried out in triplicates.NODEfalseZhenbei_sewater_16s rRNAZhenbei_sewater_16s rRNA2024-10-102024-10-09OEX00029343OEP00005639