ENAapplication/xmlftp.sra.ebi.ac.uk/vol1/fastq/ERR144/001/ERR1443011/ERR1443011.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/ERR144/002/ERR1443012/ERR1443012.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/ERR144/008/ERR1443008/ERR1443008.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/ERR144/000/ERR1443010/ERR1443010.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/ERR144/003/ERR1443013/ERR1443013.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/ERR144/009/ERR1443009/ERR1443009.fastq.gzprimaryOK2000000GenomicsCUBE - Division of Computational Systems Biology, Dept. of Microbiology and Ecosystem Science, University of Vienna, Austriahttps://www.ebi.ac.uk/ena/browser/view/PRJEB14311ArrayExpress"RNA-Seq analyses were performed in N. meningitidis strain Z5463 by deep sequencing using Ion Torrent technology (Thermo Fischer Scientific). For whole transcriptome libraries (mRNA+sRNA), cDNA libraries were prepared using the Ion Total RNA-Seq Kit v2 (Life Technologies) including a prior step of ribodepletion using specific probes adapted to N. meningitidis (Low Input Ribo Minus Eukaryote System v2) and size fractionation of RNA prior to cDNA synthesis with RNase III was performed. Briefly, 500 ng of total RNA were ribodepleted and then fragmented. After fragmentation, mean size of fragment was around 120 nt. Coding_and_NonCoding_R1, Coding_and_NonCoding_R2, and Coding_and_NonCoding_R3 represent three technical replicates.ENAribonucleic acid, Acid, Vascular Endothelial Cells, Endothelial Cells, RNA, Ribonucleic, Lymphatic Endothelial., Capillary Endothelial Cells, ribose nucleic acid, Vascular Endothelial Cell, Non Polyadenylated RNA, ribonucleic acids, Non-Polyadenylated, RNA-seq, RNS, Ribonucleic Acid, Capillary, Endothelial, Lymphatic Endothelial Cells, Cell, Vascular Endothelial, Non-Polyadenylated RNA, Capillary Endothelial, Lymphatic Endothelial, epithelial, Lymphatic, Vascular, yeast nucleic acid, Endothelial Cell, Ribonukleinsaeure, Cells, Gene Products, Whole Transcriptome Shotgun Sequencing, pentosenucleic acids, Ribonucleic acids, Capillary Endothelial Cell, Lymphatic Endothelial Cell, Non Polyadenylated, RNA Gene Products, epitheliocyteribonucleic acid, Acid, Vascular Endothelial Cells, Endothelial Cells, RNA, Ribonucleic, Lymphatic Endothelial., Capillary Endothelial Cells, ribose nucleic acid, Vascular Endothelial Cell, Non Polyadenylated RNA, ribonucleic acids, Non-Polyadenylated, RNA-seq, RNS, Ribonucleic Acid, Capillary, Endothelial, Lymphatic Endothelial Cells, Cell, Vascular Endothelial, Non-Polyadenylated RNA, Capillary Endothelial, Lymphatic Endothelial, epithelial, Lymphatic, Vascular, yeast nucleic acid, Endothelial Cell, Ribonukleinsaeure, Cells, Gene Products, Whole Transcriptome Shotgun Sequencing, pentosenucleic acids, Ribonucleic acids, Capillary Endothelial Cell, Lymphatic Endothelial Cell, Non Polyadenylated, RNA Gene Products, epitheliocyte0.00.00.00.00.00falseTwo RNA-seq studies, one of coding and the other of coding and non-coding RNA for Identification of meningococcal factors required for colonization of epithelial and endothelial cells by high throughput screeningTwo RNA-seq studies, one of coding and the other of coding and non-coding RNA for Identification of meningococcal factors required for colonization of epithelial and endothelial cells by high throughput screening2016-06-072016-07-25PRJEB14311487