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Staphylococcus aureus (MRSA) is one of the most common healthcare-associated pathogens. To examine the role of inter-hospital patient sharing on MRSA transmission, a previous study collected 2,214 samples from 30 hospitals in Orange County, California and showed by spa typing that genetic differentiation decreased significantly with increased patient sharing. In the current study, we focused on the 986 samples with spa type t008 from the same population.METHODS:We used genome sequencing to determine the effect of patient sharing on genetic differentiation between hospitals. Genetic differentiation was measured by between-hospital genetic diversity, F ST , and the proportion of nearly identical isolates between hospitals.RESULTS:Surprisingly, we found very similar genetic diversity within and between hospitals, and no significant association between patient sharing and genetic differentiation measured by F ST . However, in contrast to F ST , there was a significant association between patient sharing and the proportion of nearly identical isolates between hospitals. We propose that the proportion of nearly identical isolates is more powerful at determining transmission dynamics than traditional estimators of genetic differentiation (F ST ) when gene flow between populations is high, since it is more responsive to recent transmission events. Our hypothesis was supported by the results from coalescent simulations.CONCLUSIONS:Our results suggested that there was a high level of gene flow between hospitals facilitated by patient sharing, and that the proportion of nearly identical isolates is more sensitive to population structure than F ST when gene flow is high.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/about/who-we-are/policies/open-access-science http://www.sanger.ac.uk/resources/downloads/bacteria/ENAMRSA bacteremias, Methicillin Resistant Staphylococcus aureus, Infection, MRSA infection, Methicillin-resistant Staphylococcus aureus (MRSA) infection, MRSA bacteremia, methicillin-resistant staphylococcus aureus infectious disease., Methicillin-resistant Staphylococcus aureus, MRSA, infection by MRSA, methicillin-resistant Staphylococcus aureus (MSSA) bacteremia, NHE6MRSA bacteremias, Methicillin Resistant Staphylococcus aureus, Infection, MRSA infection, Methicillin-resistant Staphylococcus aureus (MRSA) infection, MRSA bacteremia, methicillin-resistant staphylococcus aureus infectious disease., Methicillin-resistant Staphylococcus aureus, MRSA, infection by MRSA, methicillin-resistant Staphylococcus aureus (MSSA) bacteremia, NHE60.00.00.00.00.00falseDiversity of MRSA IIDiversity of MRSA II2016-05-202012-02-07PRJEB2686268737131280