ENAapplication/xmlftp.sra.ebi.ac.uk/vol1/fastq/ERR905/ERR905144/ERR905144_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/ERR905/ERR905143/ERR905143_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/ERR905/ERR905146/ERR905146_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/ERR905/ERR905145/ERR905145_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/ERR905/ERR905143/ERR905143_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/ERR905/ERR905144/ERR905144_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/ERR905/ERR905142/ERR905142_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/ERR905/ERR905145/ERR905145_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/ERR905/ERR905142/ERR905142_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/ERR905/ERR905146/ERR905146_2.fastq.gzprimaryOK2000000GenomicsDKFZhttps://www.ebi.ac.uk/ena/browser/view/PRJEB9511Thymic antigen presenting cells (APCs) use diverse, cell type-specific strategies of self- antigen expression and presentation to co-operatively impose central tolerance. We found that intrathymic expression of Aire is not limited to medullary thymic epithelial cells, but also occurs in thymic B cells. Aire expression in B cells results from an intrathymic ‘licensing’ process that is triggered by a cross-talk with immature CD4+ thymocytes and which also elicits up-regulation of MHCII, induction of CD80 and immunoglobulin class-switching. A neo- antigen whose expression mimicked ‘licensing-associated’ self-antigen induction was directly presented by immigrating peripheral B cells for negative selection. Thus, autoreactivity within the nascent T cell repertoire fuels a tolerogenic feed forward loop that endows thymic B cells with potent APC features.ENAAIRE1, T lymphocyte tolerance induction, PGA1, T-cell tolerance induction, B-cell, B Cells, B lymphocyte, B-Lymphocyte, B Lymphocytes, Bursa-Dependent Lymphocytes, B cell, T-lymphocyte tolerance induction., APS1, Bursa-Equivalent Lymphocyte, B-lymphocyte, APSI, APECED0.00.00.00.00.00falseIntrathymic B cell licensing for Aire expression and T cell tolerance induction2016-05-202015-07-01PRJEB951110090