ENA0000GenomicsMultiomicsBiostatistics, Virginia Commonwealth Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA111363Homo sapiensBacillus anthracis is a gram-positive, aerobic, spore-forming, rod-shaped bacterium which has recently been used as an agent of bioterrorism. Because there is a significant delay between the initial contact of the spore with the host and clinical evidence of disease, there appears to be temporary containment of the pathogen by the innate immune system. Contact with the human alveolar macrophage (HAM) plays a key role in the innate immune response to B. anthracis spores. Therefore, the early macrophage response to anthrax exposure is important in understanding the pathogenesis of this disease. The majority of genes modulated by spores were upregulated, and a lesser number were downregulated. The data was subjected to Ingenuity Pathway analysis, the Database for Annotation, Visualization and Integrated Discovery (DAVID) analysis, and the Promoter Analysis and Interaction Network Toolset (PAINT). Among the upregulated genes, we identified a group of chemokine ligands, apoptosis genes and, interestingly, keratin filament genes. Central hubs regulating the activated genes were TNF-alpha, NF-κB and their ligands/receptors. Other activated genes included IL-1alpha and IL-18. RNA for these, and several additional cytokines including IL-6, IL-1gamma, IP-10 and GM-CSF, were differentially expressed from 1.6- to 27-fold. The microarray cytokine data is consistent with our previously published findings which demonstrated that there was 4- to 43-fold induction of these cytokines at the transcriptional and translational levels as determined by RNase protection assays and ELISA. The PAINT analysis revealed that the majority of the genes affected by spores contain the binding site for c-Rel, a member of the NF-κB family of transcription factors. Other transcription regulatory elements contained in many of the upregulated genes were c-Myb, CP2, Barbie Box, E2F and CRE-BP1. This study is the first detailed microarray analysis to describe the HAM response to B. anthracis. Overall design: In this paper, we exposed HAM to B. anthracis Sterne spores at an MOI of 1 for 6 hours. RNA was extracted from HAM and analyzed by the Affymetrix Human Genome U133 Plus 2.0 Array. The transcriptional profile of Bacillus anthracis spore-treated HAM was compared with mock infected cells, and differentially expressed genes were identified.ENAGene Expression Monitoring, Profiling, Transcriptome, human being, Transcriptome Profiling, Spore., Analyses, mRNA, Monocyte Derived Macrophages, Macrophages, Transcriptome Profilings, Monitorings, Gene, Transcriptomics, Bone Marrow Derived Macrophages, Bone Marrow-Derived Macrophage, Differential Displays, Monocyte Derived, Transcript Expression, Transcriptome Analysis, man, Transcriptome Analyses, human, Monitoring, Gene Expression Profilings, Bone Marrow-Derived, Gene Expression, Differential Display, Transcript Expression Analysis, transcription profiling, Gene Expression Analysis, mRNA Differential Displays, Monocyte-Derived, Transcript Expression Analyses, Gene Expression Analyses, Gene Expression Pattern Analysis, Bone Marrow-Derived Macrophages, Monocyte-Derived Macrophage, Gene Expression Monitorings, Expression Analysis, Analysis, Profilings, Expression Analyses, mRNA Differential Display, gene expression profiling, Monocyte-Derived Macrophages, Macrophagehuman being, human., man0.00.00.00.00.00falseHomo sapiensGene expression profiling of human alveolar macrophages infected by B. anthracis spores2022-05-122014-02-11PRJNA111363GSE14390197443339606