ENA0000Genomicsde Duve Institutehttps://www.ebi.ac.uk/ena/browser/view/PRJNA111445Homo sapiensThe aim of this work was to identify functional features that are specific of human Treg cells, through the identification of genes that are differentially expressed: 1/ in activated Treg clones versus activated Thelper clones; 2/ in Th clones activated in the presence versus the absence of TGFb; 3/ in suppressed Th clones, i.e. Th clones activated in the presence of Treg clones, versus controls. Keywords: TCR activation Overall design: Two to four million cells of human T cell clones were collected at rest (time point = 0) or after activation with coated anti-CD3 and soluble anti-CD28 antibodies (time points = 6, 24 or 41hrs). RNA was extracted for hybridization on Affymetrix microarrays.ENAalpha-beta regulatory T-lymphocyte, suppressor T-cell, regulatory T lymphocyte, suppressor T-lymphocyte, human being, Treg, suppressor T lymphocyte, alpha-beta regulatory T-cell, Experiment., regulatory T-lymphocyte, alpha-beta regulatory T lymphocyte, CD4-positive, CD25-positive, regulatory T-cell, man, human, suppressor T cellhuman being, human., man0.00.00.00.00.00falseHomo sapiensComparison of stable human Treg and Th clones by transcriptional profiling - experiment I2022-05-122014-02-11PRJNA111445GSE14330192246389606