ENA0000GenomicsUniversity of Washingtonhttps://www.ebi.ac.uk/ena/browser/view/PRJNA120035Mus musculusWe noticed that ThPOK repression is readily abrogated upon in vitro TCR stimulation of peripheral CD8 T cells. This observation prompted us to investigate a role of ThPOK in the CD8 T cell response to an acute viral infection. We observed that clonal expansion is significantly less in both primary and secondary CD8 T cell responses in the absence of functional ThPOK. To approach this mechanism, we carried out a microarray analysis for comparison of gene expression between ThPOKhd/hd and ThPOKwt/wt P14 memory T cells. Overall design: To identify genes either directly or indirectly regulated by ThPOK in CD8 T cells, we performed a microarray analysis of ThPOKhd/hd and ThPOKwt/wt memory P14 T cells. We analyzed gene expression profiles of memory cells since they showed the highest and most uniform ThPOK expression compared to not only naïve but also effector CD8 T cells. The two memory subsets were FACS purified during the memory phase, more than 100 days after LCMV infection.ENAMTS1, SCP, ZFP-67., Liver-type fatty acid-binding protein, hcKROX, SCP., INK4, Calgranulin-B, MAPKSP1AP, Gene, GAGB, Gene Expression Profilings, Transcript Expression Analysis, mRNA Differential Displays, Gene Expression Pattern Analysis, Z-protein, MIF, Gene Expression Monitorings, SF3B14, ARF, Analysis, Profilings, c-KROX, HSPC175, LIAG, Zfp67, AW546964, L1Ag, reference sample, Analyses, Leukocyte L1 complex heavy chain, Fatty acid-binding protein 1, MTS-1, Transcriptomics, c-Krox, Transcript Expression, Transcriptome Analysis, SF3B14a, Fabplg, Myeloid-related protein 14, Monitoring, 60B8Ag, L-FABP, Gene Expression Analysis, ZBTB15, L1AG, SUB1 homolog, CMM2, ZFP67, Migration inhibitory factor-related protein 14, P14, Squalene- and sterol-carrier protein, Ragulator2, P16, P15, Expression Analyses, P19, P16-INK4A, 60B8AG, Controlled, MAPBPIP, CKROX, Profiling, Controlling, TP16, Transcriptome, P16INK4, MLM, Monitorings, p14, ROBLD3, DOC-1R, Cell, PC4, Gene Expression, Differential Display, transcription profiling, Transcript Expression Analyses, CAGB, Ht006, P14ARF, S100 calcium-binding protein A9, mRNA Differential Display, gene expression profiling, Calprotectin L1H subunit, P16INK4A, Gene Expression Monitoring, CGLB, ZNF857B, Cagb, SAP14a, Transcriptome Profiling, mRNA, Transcriptome Profilings, MRP14, CDK4I, Positive cofactor 4, SAP14, CFAG, Differential Displays, ENDAP, Transcriptome Analyses, HSPC003, BEE22, Gene Expression Analyses, MRP-14, Thpok, NIF, P19ARF, Expression Analysis, THPOK, INK4A, MAC387, Single-stranded DNA-binding protein p9, CDKN2mouse, mouse <Mus musculus>, house mouse.0.00.00.00.00.00falseMus musculusGene expression profiling from memory P14 T cells with control or mutated ThPOK2022-05-122014-02-11PRJNA120035GSE178121973423010090