ENA0000GenomicsJohn Perfect, Medicine, Duke University Medical Centerhttps://www.ebi.ac.uk/ena/browser/view/PRJNA120071Cryptococcus neoformans var. grubii H99The Cryptococcus neoformans NRG1 gene was identified using gene microarrays to define putative transcription factor genes regulated by the cyclic AMP (cAMP) signal transduction pathway. Disruption of NRG1 results in delayed capsule formation and mating, two phenotypes that are directly controlled by cAMP signaling. Putative targets of the Nrg1 transcription factor were identified using a second genome microarray to define differences in the transcriptomes of the wild-type and nrg1 mutant strains. These experiments implicate Nrg1 in the transcriptional control of multiple genes involved in carbohydrate metabolism and substrate oxidation, as well as the UGD1 gene encoding a UDP-glucose dehydrogenase required for polysaccharide capsule production and cell wall integrity. In addition to being under transcriptional control of the cAMP pathway, Nrg1 contains a putative protein kinase A phosphorylation site; mutation of this motif results in reduced Nrg1 activity. Consistent with prior studies in hypocapsular mutants, the nrg1 mutant strain is attenuated in an animal model of disseminated cryptococcal disease. Overall design: WT and nrg1 mutant RNA were each compared to a Reference Pool RNA sample in a modified Reference design. Three hybridizations were performed for the WT and nrg1 mutant samples, and the dyes were swapped for the third hybridization of both WT and nrg1 mutant.ENAdisruption by symbiont of host cell, activation by organism of non-apoptotic programmed cell death in other organism, nrg1-A, Debaryomyces neoformans, hemolysin activity, regulation by symbiont of host system process, GGFII, modulation by organism of defense response of other organism involved in symbiotic interaction, positive regulation by symbiont of host non-apoptotic programmed cell death, NRG-1, heregulin, ARIA, Cryptococcus neoformans var. grubii, positive regulation by symbiont of host programmed cell death, Lipomyces neoformans, enhancement of host programmed cell death, induction by organism of non-apoptotic programmed cell death in other organism during symbiotic interaction, Hgl, HGL, GGF2, hemolysis by symbiont of host RBCs, modulation by symbiont of host system process, modulation by symbiont of host defense response, pathogenesis, induction by organism of programmed cell death in other organism involved in symbiotic interaction, mitigation by symbiont of host defense response, Saccharomyces neoformans, MST131, Torula neoformans, haemolysis in host, upregulation by symbiont of host programmed cell death, Torulopsis neoformans., Filobasidiella neoformans, induction of non-apoptotic programmed cell death by other organism, HRGalpha, modification by symbiont of host biological process, HRGA, enhancement of host programmed cell death by organism, stimulation by symbiont of host programmed cell death, MSTP131, NDF, perturbation by symbiont of host defense response, D230005F13Rik, induction by organism of programmed cell death in other organism during symbiotic interaction, activation by organism of programmed cell death in other organism during symbiotic interaction, modification by symbiont of host morphology or physiology, NRG1-IT2, regulation of cytolysis of host cells by symbiont, activation by organism of host programmed cell death, Blastomyces neoformans, GGF, induction by symbiont of host programmed cell death, hemolysis by symbiont of host red blood cells, activation by symbiont of host programmed cell death, Filobaxidiella neoformans, 6030402G23Rik, HRG1, cytolysis by organism of host cells, SMDF, HRGCryptococcus neoformans var. grubii serotype A H99, Cryptococcus neoformans H99.0.00.00.00.00.00falseCryptococcus neoformans var. grubii H99Effect of Nrg1 on pathogenesis of Cryptococcus neoformans2022-05-122014-02-11PRJNA120071GSE1788716835458235443