ENA0000GenomicsGUDMAP Database Group, IGMM MRC Human Genetics Unithttps://www.ebi.ac.uk/ena/browser/view/PRJNA123459Mus musculusThe long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. Overall design: At E18.5, pups were isolated from CRYM-BAC transgenic mice and fostered to CD-1 mice. Cap mesenchyme cells were isolated from the kidney by fluorescent activated cell sorting (FACS) on postnatal days 0, 1, 2, 3, and 4. RNA was isolated from FACS sorted cells and the gene expression profiles were determined by microarrays.ENANEWBORN (0-27 DAYS), Neonatal, 3.4.22.-, Laboratory, Mus domesticus, mouse, Gene, mini-ICE, LACS 1, CASP-14, condensed mesenchyme, House Mouse, Facl2, House, Mus, fetal antiepileptic drug syndrome., Mini-ICE, Mus musculus domesticus, foetal AEDS, Mice, Mus musculus, cap mesenchyme, Gene Expressions, Caspase-14 subunit p10, FACS, Swiss, mice, foetal antiepileptic drug syndrome, Swiss Mouse, House Mice, Swiss Mice, Caspase-14 subunit p19, MICE, Expressions, FAC sorting, Laboratory Mice, domesticus, LACS1, THBP, fetal AEDS, stage I nephron, Acas, Acs, Newborn, TG, Mouse, Expression, DFNA40, Laboratory Mouse, Acas1mouse, mouse <Mus musculus>, house mouse.0.00.00.00.00.00falseMus musculusGene expression profiles of cap mesenchyme and renal vesicle isolated between P0-P4 from Crym-EGFP neonatal transgenic mice using FACS. (GUDMAP Series ID: 28)2022-05-122014-02-11PRJNA123459GSE171392193179110090