ENA0000GenomicsMultiomicsAISThttps://www.ebi.ac.uk/ena/browser/view/PRJNA125233Mus musculusA functional interaction between peroxisome proliferator-activated receptor alpha (PPARalpha) and components of the circadian clock has been suggested; however, it remains to be clarified whether those transcriptional factors interact with each other to regulate the expression of their target genes. In this study, we used a ligand of PPARalpha, bezafibrate, to search the PPARalpha-regulated genes that express in a CLOCK-dependent circadian manner. Microarrays analyses using hepatic RNA isolated from bezafibrate treated-wild type, Clock mutant (Clk/Clk), and PPARalpha-null mice revealed that 136 genes are transcriptionally regulated by PPARalpha in a CLOCK-dependent manner. Overall design: Clk/Clk mutant mice with Jcl:ICR background, wild-type mice with the same strain, and PPARalpha-null mice aged 6-12 weeks were housed under a 12 h light-12 h dark cycle [LD 12:12; lights on at Zeitgeber time (ZT) 0]. For chronic treatment of bezafibrate, mice were provided with either a normal diet or the same diet containing 0.5% w/w bezafibrate for 5 days. A white fluorescent lamp provided light (300 - 500 lux at cage level) during the day. To examine the transient effect of bezafibrate injection on hepatic gene expression, bezafibrate was dissolved in warm (~40 C) sterile corn oil (Sigma) at a concentration of 10 mg/ml and administered intraperitoneally (i.p.) in a single dose of 100 mg/kg body weight at ZT2. To examine the PPARalpha-regulated genes that express in a CLOCK-dependent manner in mice, we performed oligonucleotide microarray analysis at ZT14, when CLOCK/BMAL1 transcriptional activity is maximal, using RNA isolated from wild-type (n = 3), Clock mutant (n = 3), and PPARalpha-null mice (n = 3) treated with bezafibrate for 5 days, and control wild-type mice (n = 3). Livers were collected and frozen in liquid nitrogen. Total RNA (250 ng) was extracted using RNAiso.ENAbezafibrate, data, Bezafibrat PB, bezafibrato, NR1C1, Befibrat, Bezalip, mouse, A4, PPAR, 2-(p-(2-(p-Chlorobenzamido)ethyl)phenoxy)-2-methylpropionic acid, Beza Lande, 5330400M04Rik, XClock, TYPE, LGMD2C, jecur, DAGA4, clk, BM15.075, Mus, AW742785, Peroxisome Proliferator Activated Receptor alpha, Bezabeta, Bezacur, Peroxisome Proliferator-Activated Receptor alpha, Eulitop, durabezur, Solibay, Beza Puren, Regadrin B, Bezatol SR (TN), 2-(4-(2-((4-chlorobenzoyl)amino)ethyl)phenoxy)-2-methyl-, 4933429D07Rik, MAM, Bezafisal, SCG3, bHLHe8, Difaterol, Nr1c1, hPPAR, DMDA1, Propanoic acid, mice, Bezamerck, KAT13D, Beza-Lande, PPAR-alpha, mKIAA0334, 2-{4-[2-(4-chlorobenzamido)ethyl]phenoxy}-2-methylpropanoic acid, Cedur, iecur., PPARalpha, Livers, Xclk, Béfizal, DMDA, Reducterol, Azufibrat, SCARMD2, Beza-Puren, BM-15.075, Mouse, house mouse, bezafibratum, Ppar, Befizal, Lipox, Sklerofibrat, BM 15.075mouse, mouse <Mus musculus>, house mouse.0.00.00.00.00.00falseMus musculusExpression data from (bezafibrate treated-wild type, Clock mutant, and PPARalpha-null) mouse liver2022-05-122014-02-11PRJNA125233GSE205132040068010090