ENA0000GenomicsNathan Salomonis, Biomedical Informatics, Cincinnati Children's Hospitalhttps://www.ebi.ac.uk/ena/browser/view/PRJNA134961Mus musculusLeanness is associated with increased lifespan and is linked to favorable metabolic conditions promoting life extension. We show here that deficiency of the lipid synthesis enzyme acyl CoA:diacylglycerol acyltransferase 1 (DGAT1), which reduces body fat in mice, promotes longevity. Female DGAT1-deficient mice were protected from age-related increases in body fat, non-adipose tissue triglycerides, and markers of inflammation in white adipose tissue. These metabolic changes were accompanied by an increased mean and maximal lifespan of ~25% and ~10%, respectively. The gene expression profile of DGAT1-deficient mice was not highly correlated with calorie restriction of sex and age matched wild-type littermates. Our findings indicate that loss of DGAT1-mediated lipid synthesis results in leanness, protects against age-related metabolic consequences, and thus extends longevity. Overall design: Liver gene expression profiles between short-term calorie restricted wild-type (WTCR) and Dgat1 deficient (KO) middle-aged (15-16 mo) female mice were compared to determine if calorie restriction and Dgat1 deficiency rely on common regulatory pathways for the promotion of longevity. Both CR and KO were compared to middle-aged wild-type female littermates fed a standard chow diet ad libitum (WTAL).ENA3.4.22.-, Mus musculus, Gene Expressions, DMDA1, Caspase-14 subunit p10, female human body, Laboratory, Swiss, mice, Mus domesticus, Swiss Mouse, mouse, stubby, A4, Gene, mini-ICE, House Mice, Swiss Mice, Caspase-14 subunit p19, CASP-14, MICE, Expressions, female, House Mouse, TYPE, LGMD2C, Laboratory Mice, domesticus, DAGA4, shortened, DMDA, House, Mus, SCARMD2, Mini-ICE, Mus musculus domesticus, Mouse, Expression, MAM, short, SCG3, Mice, Laboratory Mouse, Females, Mus musculus., Elderlymouse, mouse <Mus musculus>, house mouse.0.00.00.00.00.00falseMus musculusComparison of hepatic gene expression between short-term calorie restricted wild-type and Dgat1 deficient middle-aged female mice2022-05-122014-02-11PRJNA134961GSE262672229116410090