ENA0000GenomicsMultiomicsLaboratory Medicine & Genetics, Samsung Medical Centerhttps://www.ebi.ac.uk/ena/browser/view/PRJNA194409Homo sapiensA global DNA methylation and gene expression analysis of early human B-cell development reveals a demethylation signature and transcription factor network. Nucleic Acids Res. 2012 Dec;40(22):11339-51. We examined DNA methylation and RNA expression status during early B-cell development by sorting multiple replicates of four separate stages of pre-B cells derived from normal human fetal bone marrow and applied high-dimension DNA methylation scanning and expression arrays. We identified a distinct development-dependent demethylation signature which has gene expression regulatory properties for pre-B cells, and provide a catalog reference for the epigenetic changes that occur in pre-B-cell leukemia and other B-cell-related diseases. Overall design: Human fetal bone marrow was obtained, and using flow cytometry antibodies, four populations of B-cell developmental stages including stage 1 (S1; predominantly multipotent progenitors before lineage commitment and common lymphoid progenitors), stage II (S2; pre-B-I cells), stage 3 (S3; pre-B-II cells) and stage 4 cells (S4; immature B cells). For 8 indibiduals, DNAs and RNAs were isolated and the methylation and expression changes during early B-cell development were identified using the Illumina HumanMethylation450 Beadchip (Illumina) and GeneChip Human Gene 1.0 ST Array (Affymetrix).ENA0lod, data, advanced, DmelCG2684, B-cell, B Cells, human being, B Lymphocytes, B cell, Bursa-Equivalent Lymphocyte, NTef2, Lds, man, human, early, Bursa-Dependent Lymphocytes., Fs(3)Sz11, B lymphocyte, B-Lymphocyte, Horka, precocious, CG2684, Fs(3)Horka, DmF2, B-lymphocyte, Fs(3)Horhuman being, human., man0.00.00.00.00.0falseHomo sapiensExpression data from early human B-cell development2022-05-122014-02-11PRJNA194409GSE45460230741949606