ENAapplication/xmlftp.sra.ebi.ac.uk/vol1/fastq/SRR954/SRR954530/SRR954530_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR954/SRR954529/SRR954529_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR867/SRR867049/SRR867049_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR954/SRR954532/SRR954532_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR867/SRR867057/SRR867057.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR867/SRR867052/SRR867052_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR867/SRR867056/SRR867056.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR954/SRR954530/SRR954530_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR867/SRR867050/SRR867050_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR954/SRR954529/SRR954529_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR954/SRR954531/SRR954531_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR954/SRR954532/SRR954532_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR867/SRR867051/SRR867051_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR867/SRR867054/SRR867054_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR867/SRR867055/SRR867055.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR867/SRR867053/SRR867053_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR867/SRR867050/SRR867050_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR954/SRR954531/SRR954531_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR867/SRR867054/SRR867054_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR867/SRR867053/SRR867053_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR867/SRR867052/SRR867052_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR867/SRR867051/SRR867051_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR867/SRR867049/SRR867049_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR867/SRR867058/SRR867058.fastq.gzprimaryOK2000000GenomicsJiming Jiang, UW-Madisonhttps://www.ebi.ac.uk/ena/browser/view/PRJNA205183Avena sativa/Zea mays addition lineMany existing centromeres may have originated as neocentromeres that activated de novo from non-centromeric regions. However, the evolutionary path from a neocentromere to a mature centromere has been elusive. Here we analyzed the centromeres of six chromosomes that were transferred from maize into oat as the result of an inter-species cross. Centromere size and location were assayed by chromatin immunoprecipitation for the histone variant CENH3, which is a defining feature of functional centromeres. Maize and oat are highly divergent and differ in genome size by four fold. Two isolates of maize chromosome proved to contain neocentromeres in the sense that they had moved from the original site, whereas the remaining seven centromeres (1, 2, 5, 6, 8, 9 and 10) were retained in the same area in both species. In all cases the CENH3-binding domains were dramatically expanded to encompass a larger area in the oat background (~4 Mb) than the average centromere size in maize (~2 Mb). The expansion of maize centromeres appeared to be restricted by the transcription of genes located in regions flanking the original centromeres. The results from the current study provide evidence that (1) centromere size is regulated; (2) centromere sizes tend to be uniform within a species regardless of chromosome size or origin of the centromere; and (3) neocentromeres emerge and expand preferentially in gene poor regions. Our results, together with data from several animal species, suggest that centromere size expansion may be a key factor in the survival of neocentric chromosomes in natural populations. Overall design: Using ChIP-seq with CenH3 antibody, we defined the functional centromere regions in 9 oat-maize additional lines. Two oat maize additional lines include maize chromosome 3 in oats background (OMA3.01 and neoM3). The other lines include maize chromosome 1,2,5,6,8,9, and 10, respectively.ENAHOGA, ornithine-oxo-acid transaminase activity, thyroid gland oncocytic adenoma, Zea mays var. japonica, benign oncocytoma of thyroid, benign thyroid oncocytoma, Cid[Mel], Zea mays subsp. ceratina, B-cell receptor complex, AI194874, CENP-A, benign oncocytoma of the thyroid gland, Avena sativa L., CENP-C, ornithine 5-aminotransferase activity, CenpA, antibody, rOAT, BCR complex, ornithine-oxo-acid aminotransferase activity, ornithine--keto acid aminotransferase activity, Zea mays subsp. tunicata, oncocytic adenoma of the thyroid, immunoglobulin complex, follicular adenoma of thyroid gland of oxyphilic cell type, CG13329, CenH3[CID], CenH3[Cid], ornithine:alpha-oxoglutarate transaminase activity, F6F3_17, BcDNA:RE21270, membrane bound, Indian corn, DmelCG13329, DmelCG8782, F6F3.17, Hurthle cell adenoma of thyroid, ornithine ketoacid aminotransferase activity, ornithine(lysine) transaminase activity, maize, Zea mays subsp. ramosa, Cenp-A, ornithine--2-oxoacid aminotransferase activity, ornithine--alpha-ketoglutarate aminotransferase activity, L-ornithine:2-oxo-acid aminotransferase activity, B-lymphocyte receptor complex, Zea mays subsp. indurata, Zea mays subsp. amylacea, corn, thyroid follicular adenoma of the oxyphilic cell type, cultivated oat, thyroid follicular adenoma of oxyphilic cell type, GabT, benign thyroid gland oncocytoma, ornithine--keto acid transaminase activity, B lymphocyte receptor complex., cenH3, 151832_at, antibodies, oncocytic adenoma of thyroid gland, Hurthle cell adenoma, CID, Cid, Zea mays var. sacharata, ornithine transaminase activity, Zea mays subsp. everta, oncocytic adenoma of the thyroid gland, follicular adenoma of the thyroid of the oxyphilic cell type, Hurthle cell adenoma of the thyroid, ornithine delta-transaminase activity, follicular adenoma of thyroid of oxyphilic cell type, cenpA, Zea mays mays, opsonin activity, oncocytic adenoma of thyroid, OAT, Zea mays subsp. sacharata, ornithine aminotransferase activity, CENH3, CenH3, CenH3/CID, CG8782, CENP-A/Cid, CENP-A/CID, CENPA, follicular adenoma of the thyroid gland of the oxyphilic cell type, CENTROMERIC HISTONE H3, L-ornithine 5-aminotransferase activity, L-ornithine aminotransferase activity, OATASE, ornithine--ketoglutarate aminotransferase activity, thyroid gland follicular adenoma of oxyphilic cell type, benign oncocytoma of the thyroid, thyroid gland Hurthle cell adenoma, thyroid gland follicular adenoma of the oxyphilic cell type, oat, immunoglobulin, B cell receptor accessory molecule complex, GACR, Hurthle cell adenoma of the thyroid gland, Hurthle cell adenoma of thyroid gland, ornithine--oxo-acid transaminase activity, B cell receptor activity, Zea mays var. mays, thyroid Hurthle cell adenoma, Zea ramosa, OKT, CenpA/CID, L-ornithine:alpha-ketoglutarate delta-aminotransferase activity, thyroid oncocytic adenoma, benign oncocytoma of thyroid gland, ornithine--oxo acid aminotransferase activity, CID/CENP-A0.00.00.00.00.00falseAvena sativa/Zea mays addition lineChIP-seq in oat-maize additional lines using CenH3 antibody2022-05-122013-09-01PRJNA205183GSE47342241000791336713