ENA0000GenomicsMultiomicsDepartment of Genetics, University of Melbournehttps://www.ebi.ac.uk/ena/browser/view/PRJNA221201Talaromyces marneffeiPenicillium marneffei (Talaromyces marneffei) is an opportunistic human pathogen that can grow in a multicellular hyphal form at 25°C or a unicellular fission yeast form at 37°C, and can switch between these two forms in response to temperature. The yeast form is found in infected individuals and represents the pathogenic phase. In response to specific environmental cues, the hyphal form can undergo asexual development (conidiation) the produce a differentiated multicellular structure called a conidiophore which produces dormant spores (conidia). Inhaled conidia initiate infection. To identify genes that are ‘phase or cell-state specific’ transcriptional profiling was performed with a custom microarray consisting of ~42% of the predicted P. marneffei genes and RNA from P. marneffei hyphal, yeast and conidiation cell types. Overall design: A custom microarray consisting of short, random genomic fragments from P. marneffei (~42% of the predicted genes) was generated using PCR products of the inserts from two independent DNA libraries constructed from genomic DNA of the P. marneffei type strain FRR2161. PCR products from previously cloned P. marneffei genes with known expression profiles were included as controls on the microarray. Total RNA from hyphal, yeast or conidiation cell types was prepared and used in pairwise combinations (conidiation versus hyphal cells, conidiation versus yeast cells and hyphal versus yeast cells) on the microarrays. Three biological replicate cultures were prepared for each experiment.ENAProfiling, dTAF[[II]]230, TAF[[II]]250, d230, Penicilliums, Transcriptome, Monitorings, TAF200, Gene, l(3)84Ab, dTAFII250, BG:DS00004.13, TAFII-250, TAF250/230, EfW1, Cell, Gene Expression Profilings, dTAF230, Gene Expression, Differential Display, Transcript Expression Analysis, transcription profiling, dmTAF[[II]]230, mRNA Differential Displays, TAFII250, Transcript Expression Analyses, Gene Expression Pattern Analysis, dmTAF1, Taf230, p230, Gene Expression Monitorings, TAF[[II]]250/230, TFIID, Analysis, Profilings, mRNA Differential Display, gene expression profiling, TAF250, Taf[[II]]250, Gene Expression Monitoring, Yeast, Taf200, dTAF[[II]]250, TAF[[II]]230, Transcriptome Profiling, TFIID TAF250, Analyses, mRNA, cel, cell, Transcriptome Profilings, Transcriptomics, Taf1p, TAF[II]250, Differential Displays, Transcript Expression, Transcriptome Analysis, CG17603, TAF[[II]], Transcriptome Analyses, Monitoring, dTAF250, Gene Expression Analysis, DmelCG17603, Gene Expression Analyses, TAF1., Taf250, SR3-5, Expression Analysis, Expression Analyses, TAF, Eupenicillium, TAF230Penicillium marneffei.0.00.00.00.00.00falseTalaromyces marneffeiPenicillium marneffei: Gene expression profiling of hyphal and yeast cells and during conidiation2022-05-122014-02-11PRJNA221201GSE511092406253037727