ENA0000GenomicsCHU Rangueil, Team 9- I2MC, INSERM U1048https://www.ebi.ac.uk/ena/browser/view/PRJNA231198Mus musculusTo further study gene expression regulated by 17b-estradiol in uteri, we have employed whole gene microarray profiling to identify genes specifically regulated by the Estrogen receptor ERα localized either at the plasma membrane or in the nucleus. Two mice models have been used, the mice named ERa-AF2° (named AF2_KO and on C57BL6/J background) in which 7 aminoacids have been deleted in the AF2 domain and the mice C451A-ERa (named MISS_KO and on C57BL6/N background) mutated for the C451 into Ala on ERa. Control littermates were used for each mutant mice respectively name AF2_WT or MISS_WT. Overall design: 17b-estradiol induced gene expression in uteri from ovariectomized mice was measured after 6 hours exposure to 0 (PLB) and 8µg/kg of 17b-estradiol (E2) injected subcutaneously in castor oil.ENAEstraderm TTS, membrane, 17 beta Oestradiol, Estradiol Anhydrous, Ovocyclin, 17 alpha)-Isomer, Estradiol Valerate, integral to membrane, Gene, ESTRADIOL, Estra, Estradiol-17beta, membranous organ component, Estra-1, estradiol receptor, oestradiol, 17beta-estra-1, integral component of membrane, Progynon-Depot, ER, (17beta)-estra-1, whole membrane, (9 beta, 3, estra-1, beta-Estradiol, 17 beta)-Isomer, 17 beta)-(+-)-Isomer, (17-alpha)-Isomer, Estradiol Valeriante, 17 beta-Estradiol, (+-)-Isomer, 5(10)-triene-3, 17beta-oestradiol, transmembrane, membrane of organ, Estradiol 17 alpha, Gene Expressions, Estradiol Hemihydrate, (16 alpha, Progynon Depot, Estradiol, NR3A1, membrane region, Vivelle, Monosodium Salt, 17 beta-Oestradiol, Estradiol 17 beta, Estrace, 17-diol (17beta)-, nuclear receptor subfamily 3 group A member 1., (-)-Isomer, (17 alpha)-Isomer, Oestradiol, Expressions, Estradiol 17beta, Estradiol-17 alpha, Delestrogen, 17beta-estradiol, 17beta-diol, cis-estradiol, Sodium Salt, ESR, Estradiol-17 beta, 17beta oestradiol, Estr, ESR1, 17 beta Estradiol, region of membrane, Expression, Aerodiol, Progynova, 17-diol, ER-alpha, (8 alpha, Estradiol Monohydratemouse, mouse <Mus musculus>, house mouse.0.00.00.00.00.00falseMus musculusGene expression signatures in uteri from nuclear and membrane effects of Estradiol on Estrogen receptor ERα2022-05-122014-07-01PRJNA231198GSE532372437130910090