ENA0000GenomicsMultiomicsInternal Medicine / Oncology, Washington University in St. Louis School of Medicinehttps://www.ebi.ac.uk/ena/browser/view/PRJNA238299Mus musculusNK cells develop in the bone marrow and complete their maturation in peripheral organs, but the molecular events controlling maturation are incompletely understood. Utilizing an NK cell-specific miR-15/16 deficient genetic model (15aKO), we identified a critical role for miR-15/16 family microRNAs in the normal maturation of NK cells in vivo, with a specific reduction in mature CD11b+CD27- NK cells in multiple tissues. The mechanism responsible was a block in differentiation, since accelerated NK cell death was not evident, and earlier intermediates of NK cell maturation were expanded. Further, we identified Myb as a direct target of miR-15/16 in NK cells, with Myb expression increased in immature 15aKO NK cells. Following adoptive transfer, immature 15aKO NK cells exhibited defective maturation, which was rescued by ectopic miR-15/16 expression or Myb knockdown. Moreover, Myb overexpression resulted in defective NK cell maturation. Thus, miR-15/16 regulation of Myb controls the normal NK cell maturation program. Overall design: 3 technical replicates each of CD27+ 15a/16-1FKO NK cells, and CD27+ Ctrl NK cellsENAc-myb_CDS, small temporal RNA, Controlling, c-myb, Dm Myb, xmyb, reference sample, Dm-myb, stRNA, Dmyb, micro RNA, MYB, INSDC_feature:ncRNA, dMyb, cmyb, l(1)XVI, dmMYB, INSDC_qualifier:miRNA, c-Myb, NK cell., Dm myb, large granular lymphocyte, AI550390, efg, CG9045, p85, Dm-Myb, DMyb, Cmyb, null cell, M16449, myb, DmelCG9045, microRNA, D-myb, DmMyb, DmMYB, dmyb, Controlledmouse, mouse <Mus musculus>, house mouse.0.00.00.00.00.00falseMus musculusMicroRNA-15/16 Antagonizes c-Myb to Control Natural Killer Cell Maturation2022-05-122015-09-05PRJNA238299GSE550332626865710090