ENAapplication/xmlftp.sra.ebi.ac.uk/vol1/fastq/SRR129/001/SRR1292301/SRR1292301.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/005/SRR1292295/SRR1292295.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/003/SRR1292293/SRR1292293.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/007/SRR1292287/SRR1292287.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/003/SRR1292303/SRR1292303.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/006/SRR1292286/SRR1292286.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/007/SRR1292297/SRR1292297.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/002/SRR1292292/SRR1292292.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/009/SRR1292289/SRR1292289.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/004/SRR1292294/SRR1292294.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/000/SRR1292300/SRR1292300.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/002/SRR1292302/SRR1292302.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/008/SRR1292288/SRR1292288.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/000/SRR1292290/SRR1292290.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/009/SRR1292299/SRR1292299.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/008/SRR1292298/SRR1292298.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/001/SRR1292291/SRR1292291.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR129/006/SRR1292296/SRR1292296.fastq.gzprimaryOK2000000GenomicsMultiomicsFunctional Genomics, IGBMChttps://www.ebi.ac.uk/ena/browser/view/PRJNA248144Mus musculusThe nuclear receptor HNF4A regulates embryonic and post-natal hepatocyte gene expression. Using hepatocyte-specific inactivation in mice, we show that the TAF4 subunit of TFIID acts as a cofactor for HNF4A in vivo and that HNF4A interacts directly with the TAF4-TAF12 heterodimer in vitro. In vivo, TAF4 is required to maintain HNF4A-directed embryonic gene expression at post-natal stages and for HNF4A-directed activation of post-natal gene expression. TAF4 promotes HNF4A occupancy of functional cis-regulatory elements located adjacent to the transcription start sites of post-natal expressed genes and for pre-initiation complex formation required for their expression. Promoter-proximal HNF4A-TFIID interactions are therefore required for pre-initiation complex formation and stable HNF4A occupancy of regulatory elements as two concomitant mutually dependent processes. Overall design: Examination of PIC, H3k4me3, Ctcf and Hnf4a occupancy in wild-type and Taf4-/- livers by deep sequencingENAphosphoric acid tributyl ester, d110, TAF48, CPD photolyase activity, GTF2D, l(3)72Dj, PhrB photolyase activity, NR2A1, Gene, Hepatocyte, HNF4, Hnf4, biosynthesis, photoreactivating enzyme activity, pigmented epithelium, nr2a1, deoxyribodipyrimidine photolyase activity, stratum pigmentosum retinae, element, DmelCG5444, RBP, hnf4, NR2A21, Taf110, dmTAF4, 3, TAF2C, stratum pigmentosa retinae, Gtf2d, NUP96, outer pigmented layer of retina, MODY1, TBP38, epithelium, multicellular organismal biosynthetic process, Nr2a1, DmelCG9874, single-organism biosynthetic process, HDL4, MOS3, deoxyribocyclobutadipyrimidine pyrimidine-lyase activity, Gene Expressions, FRTS4, PRECOCIOUS, formation, anabolism, SPAC30.12, pigmented retina, F23A5.3, Expressions, DNA cyclobutane dipyrimidine photolyase activity, SUPPRESSOR OF AUXIN RESISTANCE 3, synthesis, HNF4a7, HNF4a8, MPT1, HNF4a9, pigment epithelium of retina, SCA17, TFIID 110, GTF2D1, pigmented retina epithelium, Hepatic, SY3-2 TAF110, Expression, elements, TAF4A, TAF, TFIIDtau, F23A5_3, atom, serum total protein, Hepatic Cell., Taf[[II]]110, PRE, dTAF[[II]]110, deoxyribonucleic cyclobutane dipyrimidine photolyase activity, tri-(n-butyl )-phosphate, dTBP, dTbp, TAF[II]110, p110, MODY, CG5444, dTAF4, pigmented retinal epithelium, TfIID, Cell, Hepatic Cells, TBP, TAFII130, stratum pigmentosum (retina), MODIFIER OF SNC1, TCF14, retinal pigment, TAFII135, Hnf4alpha, TSG2, taf110, deoxyribonucleic photolyase activity, dipyrimidine photolyase (photosensitive), TFIID, atomo, atomus, TCF, atome, retinal pigment layer, HNF-4, activation, HNF4alpha, tbp, TAF[[II]]110, RPE, Element, tri-n-butyl phosphate, tributylphosphate, photolyase activity, tdf1, retinal pigmented epithelium, atoms, tri-n-butylphosphate, initiation, Tcf14, dTAFII110, TAF[[II]], retinal pigment epithelium, p. pigmentosa retinae, phr A photolyase activity, TAFII110, DNA-photoreactivating enzyme, dTFIID, TAF2C1, SR3-3, Cells, dtbp, deoxyribonucleate pyrimidine dimer lyase (photosensitive), CG9874, TAF110, TAF4mouse, mouse <Mus musculus>, house mouse.0.00.00.00.00.00falseMus musculusTAF4 promotes pre-initiation complex formation and HNF4A occupancy of regulatory elements required to activation post-natal gene expression programme in hepatocytes (ChIP-seq)2022-05-112014-09-27PRJNA248144GSE578072520999710090