ENA0000GenomicsMultiomicsLaboratory of Cell Proliferation and Ageing, Institute of Biosciences and Applications, NCSR "Demokritos"https://www.ebi.ac.uk/ena/browser/view/PRJNA255415Bos taurusAssessment of the putative differential gene expression profiles in high osmolality-treated bovine nucleus pulposus intervertebral disc cells for a short (5 h) and a long (24 h) time period. Identification of novel genes up- or down-regulated as an early or a late response to hyperosmotic stress. A 5 and 24 h-hyperosmotic treatment of nucleus pulposus cells led to transcriptional changes in >100 and 200 genes, respectively. Overall design: Nucleus pulposus intervertebral disc cells were exposed to hyperosmotic stress for 5 and 24 h before RNA extraction and transcriptomics analysis. Three biological replicates were tested for each condition. Selected genes found to be differentially expressed were validated by RT-qPCR. Functional experiments were performed in order to assess the role of specific proteins encoded by genes found to be up-regulated in the osmo-reguatory response of intervertebral disc cells.ENAdairy cow, data, dTAF[[II]]230, TAF[[II]]250, d230, cow, TAF200, l(3)84Ab, dTAFII250, BG:DS00004.13, TAFII-250, TAF250/230, EfW1, Cell, dTAF230, dmTAF[[II]]230, TAFII250, DISC protein complex, cattle, dmTAF1, Bos primigenius taurus, Taf230, p230, TAF[[II]]250/230, disk, TFIID, domestic cattle, death-inducing signalling complex, Disco, DISC, disc, TAF250, Taf[[II]]250, Taf200, dTAF[[II]]250, TAF[[II]]230, TFIID TAF250, cel, cell, Bos bovis, discus, Taf1p, TAF[II]250, CG17603, CG9908, TAF[[II]], dTAF250, Bos Tauurus, oxen, DmelCG17603, DmelCG9908, TAF1., ox, Taf250, SR3-5, death receptor-induced signalling complex, domestic cow, death receptor-induced signaling complex, TAF, bovine, TAF230, nucleus pulposus cellBos bovis, dairy cow, Bos Tauurus, domestic cattle, domestic cow, oxen, Bos primigenius taurus., bovine, ox, cattle, cow0.00.00.00.00.00falseBos taurusExpression data from bovine nucleus pulposus interverteral disc cells2022-05-122015-06-21PRJNA255415GSE59485259673989913