ENAapplication/xmlftp.sra.ebi.ac.uk/vol1/fastq/SRR191/004/SRR1915444/SRR1915444.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR191/000/SRR1915440/SRR1915440.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR191/002/SRR1915442/SRR1915442.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR191/001/SRR1915441/SRR1915441.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR191/009/SRR1915439/SRR1915439.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR191/003/SRR1915443/SRR1915443.fastq.gzprimaryOK2000000GenomicsGenetics, STANFORD UNIVERSITYhttps://www.ebi.ac.uk/ena/browser/view/PRJNA278162Mus musculusMicroRNAs have emerged as key regulators of B cell fate decisions and immune function. Deregulation of several microRNAs in B cells leads to the development of autoimmune disease and cancer in mice. We demonstrate that the microRNA-212/132 cluster (miR-212/132) is induced in B cells in response to B cell receptor signaling. Enforced expression of miR-132 results in a block in early B cell development at the pre-pro-B cell to pro-B cell transition and induces apoptosis in primary bone marrow B cells. Importantly, loss of miR-212/132 results in increased B cell output under non-homeostatic conditions. We find that miR-212/132 regulates B lymphopoiesis by targeting the transcription factor SOX4. Co-expression of SOX4 with miR-132 rescues the defect in B cell development from over-expression of miR-132 alone. In addition, we show that the expression of miR-132 in cells that are prone to spontaneous B cell cancers can have a protective effect on cancer development. We have thus uncovered a novel regulator of B cell lineage specification that may potential applications in B cell cancer therapy Overall design: RNA-seq of wild-type and microRNA-212/132 knock-out B-cells after IgM stimulationENAcellular suicide, cell suicide, Caspase-Dependent Apoptosis, stRNA, caspase-dependent programmed cell death, apoptotic cell death, B-cell differentiation, Caspase Dependent Apoptosis, micro RNA, type I programmed cell death, apoptotic programmed cell death, activation of apoptosis, sox4., signaling (initiator) caspase activity, induction of apoptosis, Extrinsic Pathway Apoptoses, EVI16, Intrinsic Pathway, Programmed, Programmed Cell Death, Extrinsic Pathway Apoptosis, Type I, Caspase-Dependent, apoptosis signaling, small temporal RNA, B-lymphocyte differentiation, apoptosis, AA682046, Cell Death, apoptosis activator activity, INSDC_feature:ncRNA, INSDC_qualifier:miRNA, Classic Apoptoses, Intrinsic Pathway Apoptoses, Intrinsic Pathway Apoptosis, Sox-4, induction of apoptosis by p53, Classic, B cell development, Classic Apoptosis, apoptotic program, B lymphocyte differentiation, execution phase of apoptotic process, Classical Apoptosis, Extrinsic Pathway, commitment to apoptosis, microRNA, programmed cell death by apoptosis, Classical, Apoptoses, Apoptosismouse, mouse <Mus musculus>, house mouse.0.00.00.00.00.00falseMus musculusThe microRNA-212/132 cluster regulates B cell development and apoptosis by targeting SOX42022-05-122015-09-01PRJNA278162GSE668822637118810090