ENA0000GenomicsMultiomicsCBIIT, NCIhttps://www.ebi.ac.uk/ena/browser/view/PRJNA288320Homo sapiensCutaneous T-cell lymphoma (CTCL) is defined by infiltration of activated and malignant T cells in the skin. The clinical manifestations and prognosis in CTCL are highly variable. In this study, we hypothesized that gene expression analysis in lesional skin biopsies can improve understanding of the disease and its management. Based on 63 skin samples, we performed consensus clustering, revealing 3 patient clusters. Of these, 2 clusters tended to differentiate limited CTCL (stages IA and IB) from more extensive CTCL (stages IB and III). Stage IB patients appeared in both clusters, but those in the limited CTCL cluster were more responsive to treatment than those in the more extensive CTCL cluster. The third cluster was enriched in lymphocyte activation genes and was associated with a high proportion of tumor (stage IIB) lesions. Survival analysis revealed significant differences in event-free survival between clusters, with poorest survival seen in the activated lymphocyte cluster. Using supervised analysis, we further characterized genes significantly associated with lower-stage/treatment-responsive CTCL versus higher-stage/treatment-resistant CTCL. We conclude that transcriptional profiling of CTCL skin lesions reveals clinically relevant signatures, correlating with differences in survival and response to treatment. Additional prospective long-term studies to validate and refine these findings appear warranted. Overall design: kuppe-00390 Assay Type: Gene Expression Provider: Affymetrix Array Designs: U133AAofAv2 Organism: Homo sapiens (ncbitax) Tissue Sites: Skin Material Types: total RNA, synthetic_RNA, organism_part, whole_organism Disease States: Cutaneous T-cell lymphomaENAProfiling, other disease, CTCL, cutaneous T-cell lymphoma, Transcriptome, wide/broad, disorders, Monitorings, T-cell non-Hodgkin's lymphoma of skin, Gene, broad, primary cutaneous T-cell non-Hodgkin lymphoma, Gene Expression Profilings, Gene Expression, Differential Display, Transcript Expression Analysis, transcription profiling, mRNA Differential Displays, Transcript Expression Analyses, Gene Expression Pattern Analysis, diseases, Diseases, Gene Expression Monitorings, T-cell non-Hodgkin's lymphoma of the skin, disease or disorder, condition, diseases and disorders, Analysis, Profilings, mRNA Differential Display, gene expression profiling, Gene Expression Monitoring, human disease, Transcriptome Profiling, Analyses, mRNA, cutaneous T cell lymphoma, Transcriptome Profilings, skin T-cell non-Hodgkin's lymphoma, Transcriptomics, cutaneous T-cell non-Hodgkin's lymphoma, Differential Displays, Transcript Expression, Transcriptome Analysis, Transcriptome Analyses, Monitoring, non-neoplastic, cutaneous T-cell non-Hodgkin lymphoma, primary cutaneous T-cell non-Hodgkin's lymphoma, Gene Expression Analysis, disease, wide, Gene Expression Analyses, PCTCL, disorder, Homo sapiens disease, medical condition., Expression Analysis, associated, Expression Analyseshuman being, human., man0.00.00.00.00.00falseHomo sapienscaArray_kuppe-00390: Broad: Lesional gene expression profiling in cutaneous T-cell lymphoma reveals natural clusters associated with disease outcome2022-05-122015-06-28PRJNA288320GSE70328176388529606