ENA0000GenomicsLaboratory of Biology of the cell Nucleus, Depertment of Biology of the cell Nucleus, Institute of Molecular Geneticshttps://www.ebi.ac.uk/ena/browser/view/PRJNA305129Mus musculusPlasma membrane tension is an important feature that determines the cell shape and influences processes such as cell motility, spreading, endocytosis and exocytosis. Unconventional class 1 myosins are potent regulators of plasma membrane tension because they physically link the plasma membrane with the adjacent cytoskeleton. We identified nuclear myosin 1 (NM1) - a putative nuclear isoform of myosin 1c (Myo1c) - as a new player in the field. Although having specific nuclear function, we show that NM1 localizes preferentially to the plasma membrane. Deletion of NM1 causes more than 50% increase in the elasticity of the plasma membrane around the actin cytoskeleton as measured by atomic force microscopy. This higher elasticity of NM1 knock-out cells leads to 25% higher resistance to short-term hypotonic environment and rapid cell swelling. In contrary, overexpression of NM1 in WT cells leads to additional 30% reduction of their survival. We have brought evidence that NM1 has a direct functional role in the cytoplasm as a dynamic linker between the cell membrane and the underlying cytoskeleton, regulating the degree of effective plasma membrane tension. Overall design: We used tissues from NM1 WT and KO mice with highest expression of NM1, lungs and heart (3 replicates each) and skin fibroblast derived from each mice (2 replicates each)ENA3.4.22.-, Mus musculus, Caspase-14 subunit p10, Laboratory, Swiss, mice, Mus domesticus, Swiss Mouse, mouse, mini-ICE, House Mice, Swiss Mice, Caspase-14 subunit p19, CASP-14, MICE, House Mouse, Laboratory Mice, domesticus, House, Mus, Mini-ICE, Mus musculus domesticus, Mouse, Mice, Laboratory Mouse, Mus musculus.mouse, mouse <Mus musculus>, house mouse.0.00.00.00.00.00falseMus musculusExpression profiling of NM1 KO and WT mice2022-05-122015-12-06PRJNA305129GSE7570910090