ENAapplication/xmlftp.sra.ebi.ac.uk/vol1/fastq/SRR399/001/SRR3997851/SRR3997851_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR399/001/SRR3997851/SRR3997851_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR399/000/SRR3997850/SRR3997850_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR399/009/SRR3997849/SRR3997849_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR399/000/SRR3997850/SRR3997850_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR399/001/SRR3997871/SRR3997871_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR399/009/SRR3997849/SRR3997849_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR399/001/SRR3997871/SRR3997871_2.fastq.gzprimaryOK2000000GenomicsDNARamsinghLabhttps://www.ebi.ac.uk/ena/browser/view/PRJNA336432Homo sapiensLibraries for whole-genome bisulfite sequencing were generated from 5 ng of purified DNA from paired senescent and active CD34+ cells from 3 healthy humans (33, 45, and 53 years of age) using the NuGen Ovation Ultralow MethylSeq Kit following the manufacturer’s protocol. Reads were aligned using Biscuit (https://github.com/zwdzwd/biscuit) and Metilene was used forcalling of differentially methylated regions (Juhling et al., 2016b). DMRs were scanned for overlap with linked regions and the target genes used for prediction of RNA expression differences. Motif analysis of DMRs was conducted using the PWMEnrich package (http://www.bioconductor.org/packages/2.12/bioc/html/PWMEnrich.html) with Hocomoco and Factorbook motif databases provided in the motifbreakR package (Coetzee et al., 2015)ENAhematopoietic precursor cell, whole genome bisulfite sequencing, human being, hemopoietic progenitor cell, primary axis, CFU-S, hematopoietic progenitor cell, WGBS, blutbildende Stammzelle, axis, hemopoietic stem cell, progenitor cell, stalk, HSC cell, primary stem, HSC, man, human, culm, colony forming unit spleen, Shotgun bisulfite sequencing., whole-genome shotgun bisulfite sequencing, WGSBShuman being, DNS, WGBS, (Deoxyribonucleotide)n, beta Galactosidase, e(-), Dairyaid, DELTA AND GAMMA, hematopoietic progenitor cell antigen CD34, electron, betaTub1, Lactrase, Deoxyribonucleic acids, Shotgun bisulfite sequencing., B1t, beta Galactosidases, Deoxyribonucleic Acid, B-spec, betaspec, animal breed, beta D Galactosidase, Lactaid, Lactogest, whole genome bisulfite sequencing, beta Galactosidase A3, thymus nucleic acid, beta Galactosidase A1, betatub(56D), beta Galactosidase A2, kus, beta1tub, CG9277, Elektron, beta[[1]] tubulin, beta-Galactosidases, beta1t, Double Stranded, T, beta-particle, Deoxyribonucleic acid, lac Z, man, ecotype, anon-EST:fe1B3, 1t, e, e-, beta1Tub, SPEC8, Double-Stranded DNA, (Deoxyribonucleotide)m, deoxyribonucleic acids, CG5870, DNAn, whole-genome shotgun bisulfite sequencing, beta-tubulin56D, Spec-beta, Dmbeta1, BETA 56D, DNAn+1, Tubulin, Double-Stranded, i168, CD34, strain, (Deoxyribonucleotide)n+m, l(1)G0108, Protein, Lactases, beta-Galactosidase A2, beta-Galactosidase A3, betaSpec, FLOWERING TIME CONTROL PROTEIN FCA ALPHA, beta-Galactosidase A1, cultivar, ds-DNA, desoxyribose nucleic acid, b-Spec, lac Z Protein, AU040960, beta-tub, DmelCG5870, l(1)G0074, l(1)G0198, beta-D-Galactosidase, Beta <eudicots>, negatron, beta-D-Galactoside galactohydrolase, beta[[1]]-tubulin, b spectrin, FCAALL.331, beta1, beta56D, DmelCG9277, human, beta, beta1-tub, beta(-), DTB2, Tub, beta-Tub, beta-spec, i6, DL4180C, ds DNA, BETA, Desoxyribonukleinsaeure, DNA, beta-Tub56D, Spec, betaTub, Specbeta, beta1-Tubulin, WGSBS0.00.00.00.00.00falseHomo sapiens strain:DNA | isolate:SA-Beta-beta-Galactosidase-CD34-Positive | breed:Whole Genome bisulfite sequencingActive and senescent human hematopoietic stem and progenitor cell whole genome bisulfite sequencing2022-05-122016-08-05PRJNA3364329606