ENAapplication/xmlftp.sra.ebi.ac.uk/vol1/fastq/SRR645/003/SRR6452893/SRR6452893.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/000/SRR6452910/SRR6452910.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/007/SRR6452887/SRR6452887.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR518/003/SRR5182203/SRR5182203.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/005/SRR6452905/SRR6452905.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/002/SRR6452892/SRR6452892.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/001/SRR6452911/SRR6452911.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR518/009/SRR5182209/SRR5182209.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/009/SRR6452899/SRR6452899.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/006/SRR6452886/SRR6452886.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/007/SRR6452917/SRR6452917.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/004/SRR6452904/SRR6452904.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/003/SRR6452923/SRR6452923.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR518/002/SRR5182202/SRR5182202.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/006/SRR6452906/SRR6452906.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR518/004/SRR5182204/SRR5182204.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/005/SRR6452895/SRR6452895.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/006/SRR6452916/SRR6452916.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/004/SRR6452924/SRR6452924.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/004/SRR6452894/SRR6452894.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/005/SRR6452915/SRR6452915.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR518/003/SRR5182213/SRR5182213.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/008/SRR6452908/SRR6452908.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR518/002/SRR5182212/SRR5182212.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/004/SRR6452914/SRR6452914.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/007/SRR6452897/SRR6452897.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/000/SRR6452900/SRR6452900.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR518/006/SRR5182206/SRR5182206.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/003/SRR6452913/SRR6452913.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/000/SRR6452890/SRR6452890.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/007/SRR6452907/SRR6452907.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR518/001/SRR5182211/SRR5182211.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/000/SRR6452920/SRR6452920.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR518/005/SRR5182205/SRR5182205.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/001/SRR6452901/SRR6452901.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/006/SRR6452896/SRR6452896.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/002/SRR6452912/SRR6452912.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR518/008/SRR5182208/SRR5182208.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/001/SRR6452891/SRR6452891.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR518/000/SRR5182210/SRR5182210.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/009/SRR6452919/SRR6452919.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/008/SRR6452898/SRR6452898.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/001/SRR6452921/SRR6452921.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/003/SRR6452903/SRR6452903.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/002/SRR6452922/SRR6452922.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/009/SRR6452889/SRR6452889.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/008/SRR6452888/SRR6452888.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR518/007/SRR5182207/SRR5182207.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/002/SRR6452902/SRR6452902.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/008/SRR6452918/SRR6452918.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR645/009/SRR6452909/SRR6452909.fastq.gzprimaryOK2000000GenomicsNeurology and Neurosurgery, McGill Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA362200Caenorhabditis elegansMild deficits in mitochondrial function have been shown to increase lifespan in multiple species including worms, flies and mice. Here, we study three C. elegans mitochondrial mutants (clk-1, isp-1 and nuo-6) to identify overlapping genetic pathways that contribute to their longevity. We find that genes regulated by the FOXO transcription factor DAF-16 are upregulated in all three strains, and that the transcriptional changes present in these worms overlap significantly with the long-lived insulin-IGF1 signaling pathway mutant daf-2. We show that DAF-16 and multiple DAF-16 interacting proteins (MATH-33, IMB-2, CST-1/2, BAR-1) are required for the longevity of all three mitochondrial mutants. Our results suggest that the activation of DAF-16 in these mutants results from elevated levels of reactive oxygen species. Overall, this work reveals an overlapping genetic pathway required for longevity in three mitochondrial mutants, and, combined with previous work, demonstrates that DAF-16 is a downstream mediator of lifespan extension in multiple pathways of longevity. Overall design: Six biological replicates per genotype (WT, sod-2, clk-1, nuo-6, isp-1, daf-2) or three biological replicates per genotype (sod-1, ctl-1, prdx-2); mRNA was isolated individually from each replicate; Six WT and sod-2 were sequenced individually in a different experiment though were analyzed in the same way as other samplesENACG16878, Life Span, Pro Oxidants, length of life, Lifespans, Daf1, daf, Life Spans, BG:DS05899.4, Radical, BG:DS05899.5, Length of Life, Dalpha5, Oxygen Radical, CROM, Daf-TM, long., Reactive Oxygen Intermediates, nAChR, CG4498, Nic 34E, AI323748, Oxygen Species, CT14633, Oxygen, GPI-DAF, DAF, Daf, Active Oxygen Species, anon-WO02059370.44, TC, CR, Pro-Oxidants, Active, Pro Oxidant, Pro-Oxidant, DmelCG32975, Active Oxygen, CG32975, Oxygen Radicals, activation, Daf-GPI, TM-DAF, Reactive, Lifespan, Cd55bnematode, Caenorhabditis elegan., Caenorhabditis, Rhabditis elegans, elegan0.00.00.00.00.00falseCaenorhabditis elegansActivation of DAF-16/FOXO by reactive oxygen species promotes longevity in long- lived mitochondrial mutants in C. elegans2022-05-122018-03-01PRJNA362200GSE93724295225566239