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and Columbia River Inter-Tribal Fish Commission (CRITFC)https://www.ebi.ac.uk/ena/browser/view/PRJNA402052Oncorhynchus tshawytschaThe genome was sequenced with a combination of TruSeq long-read libraries, BAC libraries, mate-pair libraries (3-40kb) and BioNano optical maps. TruSeq and BAC libraries were assembled with SPADES to obtain synthetic long reads, followed by a whole genome assembly using CELERA. Resulting contigs were scaffolded using mate-pairs libraries with FAST-SG+OPERA-LG. Scaffolds were error-corrected using optical maps with BioNano Access v.1.0. Finally, scaffolds were ordered into chromosomes with available genetic maps (Brieuc et al. 2014; McKinney et al. 2015) and whole genome alignments to a reference rainbow trout genome (Palti et al. 2017). The resulting assembly has a total size of 2.36Gb with a contig N50 of 19.1Kb, scaffold N50 of 153Kb and 72% (1.7Gb) of the assembly anchored to chromosomes (N50 45.4Mb).ENASalmo tshawytscha, Oncorhynchus tschawytscha, king salmon, Chinook salmon, 1792), Oncorhynchus tshawytscha (Walbaum, Oncorhynchus tshawytsha, Genomes.Salmo tshawytscha, Oncorhynchus tschawytscha, king salmon, Chinook salmon, 1792), Oncorhynchus tshawytscha (Walbaum, Oncorhynchus tshawytsha.0.00.00.00.00.00falseOncorhynchus tshawytscha isolate:JC-2011-M1Oncorhynchus tshawytscha isolate:JC-2011-M1 Genome sequencing and assembly2022-05-122017-09-09PRJNA40205274940