ENAapplication/xmlftp.sra.ebi.ac.uk/vol1/fastq/SRR650/006/SRR6503146/SRR6503146.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR650/000/SRR6503150/SRR6503150.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR650/005/SRR6503145/SRR6503145.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR650/008/SRR6503148/SRR6503148.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR650/001/SRR6503151/SRR6503151.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR650/004/SRR6503144/SRR6503144.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR650/009/SRR6503149/SRR6503149.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR650/000/SRR6503140/SRR6503140.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR650/001/SRR6503141/SRR6503141.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR650/003/SRR6503143/SRR6503143.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR650/007/SRR6503147/SRR6503147.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR650/002/SRR6503142/SRR6503142.fastq.gzprimaryOK2000000GenomicsMultiomicsBSPII room 547, Univ. of Pittsburghhttps://www.ebi.ac.uk/ena/browser/view/PRJNA430862The abundance of HSV mRNAs was determines over 16h of infection in wt (KOS) and n199 (ICP22 mutant) infected cells. ICP22 is an immediate early gene of HSV that affects gene expression Overall design: 2 X 10E6 MRC5 cells were infected at an MOI of 10 PFU/cell. mRNA was isolated at different times post infection using Ambion RNaqueous-4PCR kit and quantified using an Agilent Eukaryotic RNA Nano kit and a 2100 bioanalyzer. Individual samples each with unit barcodes were quantified using an Agilent DNA 7500 chip an a 2100 bioanalyzer. Pooled multiplexed samples were sequenced at the Tufts University Genomics facility. The demulitplexed sequence read files were mapped to the HSV strain KOS genome and quantified using CLC Genomics Workbench.ENAocular anomalies, Herpes simplex virus type 1, blepharophimosis-ptosis-intellectual disability syndrome, dTAF[[II]]230, TAF[[II]]250, d230, kos, HSV-1, TAF200, herpes simplex virus type 1 HSV1, Kaufman type, l(3)84Ab, dTAFII250, and long thin hands and feet, BG:DS00004.13, TAFII-250, TAF250/230, EfW1, Cell, dTAF230, dmTAF[[II]]230, TAFII250, oculocerebrofacial syndrome, dmTAF1, Taf230, p230, herpes simplex virus 1 HSV-1, Herpes simplex virus 1, TAF[[II]]250/230, TFIID, TAF250, Taf[[II]]250, Taf200, HSV1, dTAF[[II]]250, TAF[[II]]230, TFIID TAF250, long narrow face, cel, cell, BPIDS, Taf1p, TAF[II]250, herpes simplex virus type 1 HSV-1, CG17603, TAF[[II]], microcephaly, dTAF250, severe intellectual disability, Kaufman oculocerebrofacial syndrome, DmelCG17603, herpes simplex virus type-1 HSV-1, severe mental retardation, TAF1., Taf250, SR3-5, herpes simplex virus HSV-1, KOS, Human Herpesvirus-1, Human herpesvirus type 1, TAF, TAF230ocular anomalies, Herpes simplex virus type 1, blepharophimosis-ptosis-intellectual disability syndrome, dTAF[[II]]230, TAF[[II]]250, d230, kos, HSV-1, TAF200, herpes simplex virus type 1 HSV1, Kaufman type, l(3)84Ab, dTAFII250, and long thin hands and feet, BG:DS00004.13, TAFII-250, TAF250/230, EfW1, Cell, dTAF230, dmTAF[[II]]230, TAFII250, oculocerebrofacial syndrome, dmTAF1, Taf230, p230, herpes simplex virus 1 HSV-1, Herpes simplex virus 1, TAF[[II]]250/230, TFIID, TAF250, Taf[[II]]250, Taf200, HSV1, dTAF[[II]]250, TAF[[II]]230, TFIID TAF250, long narrow face, cel, cell, BPIDS, Taf1p, TAF[II]250, herpes simplex virus type 1 HSV-1, CG17603, TAF[[II]], microcephaly, dTAF250, severe intellectual disability, Kaufman oculocerebrofacial syndrome, DmelCG17603, herpes simplex virus type-1 HSV-1, severe mental retardation, TAF1., Taf250, SR3-5, herpes simplex virus HSV-1, KOS, Human Herpesvirus-1, Human herpesvirus type 1, TAF, TAF2300.00.00.00.00.00falseRNAseq comparison of HSV-1 KOS vs n199 infected cellsRNAseq comparison of HSV-1 KOS vs n199 infected cells2022-05-122018-03-01PRJNA430862GSE109420286112499606