ENAGenomicsHerbalife Nutritionhttps://www.ebi.ac.uk/ena/browser/view/PRJNA503738The Herbalife Genomic Barcode Database is a collection of barcode nucleotide sequences of botanicals used in food, herbal medicine, and dietary supplements. Originally coined for using a standardized DNA region to quickly identify existing species and discover new species, DNA barcoding can also be used for species identification in botanical authentication. Sequences were collected from reference materials purchased from herbariums, organizations, or commercial entities. Sequences in the database are from one of the following 5 genetic regions – rbcL, matK (coding regions), ITS2, trnH, trnL (non-coding regions). For each species, sequences were collected from at least two genetic regions, one from a coding region (1st tier) and one from a non-coding region (2nd tier) to support a two-tiered approach in species identification. The use of a coding region in combination with a non-coding region optimizes efficiency and species discrimination power. While the coding regions are efficient in identification at genus level, the highly-variable non-coding regions are more effective for species-level identification. The sequences in the database are trimmed, with primer sequences removed. The sequences have been validated against the public database to confirm that an independent lab has reported a similar sequence from the same species. For inquiries, please contact zhengfeil@herbalife.com .ENADNA barcode, Data Base.0.00.00.00.00.0falseHerbalife Botanical Barcode Database2022-06-162018-11-06PRJNA50373830717821