ENAapplication/xmlftp.sra.ebi.ac.uk/vol1/fastq/SRR141/093/SRR14192693/SRR14192693_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR141/089/SRR14192689/SRR14192689_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR141/090/SRR14192690/SRR14192690_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR141/092/SRR14192692/SRR14192692_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR141/091/SRR14192691/SRR14192691_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR141/089/SRR14192689/SRR14192689_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR141/092/SRR14192692/SRR14192692_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR141/088/SRR14192688/SRR14192688_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR141/091/SRR14192691/SRR14192691_2.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR141/090/SRR14192690/SRR14192690_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR141/093/SRR14192693/SRR14192693_1.fastq.gzftp.sra.ebi.ac.uk/vol1/fastq/SRR141/088/SRR14192688/SRR14192688_2.fastq.gzprimaryOK200GenomicsGenetic Medicine and Development, University of Genevahttps://www.ebi.ac.uk/ena/browser/view/PRJNA720700Mus musculusTo compare expression profiles of fetal and adult mouse Leydig cells, we performed bulk RNA sequencing. Overall design: Mouse testis from XY Nr5a1 Tg mouse embryos at embryonic day (E) 18.5 and 100-day old mice were harvested. Tissues were enzymatically dissociated and then filtered.The single cell solution is then sorted by fluorescent-active cell sorting to obtain a solution of alive and single Nr5a1-GFP+ cells. RNA was extracted from these cells with RNeasy Mini kit (Qiagen). Sequencing libraries were prepared with the TruSeq Stranded Total RNA Library Prep Gold (Ribo-Zero) and sequenced on an Illumina HiSeq 2500 (50 bp, paired end, ~35 million reads expected). Each condition was performed in three independent replicates.ENALeydig Cell, adults, adult stage, Cells, Interstitial Cell, Interstitial Cells, Testicular Interstitial Cells, Whole Transcriptome Shotgun Sequencing, Testicular Interstitial, Testicular Interstitial., RNA-seq, Dietary Fiber, Adults, Leydig, adult, Testicular, Cell, Testicular Interstitial CellLeydig Cell, adults, adult stage, Cells, Interstitial Cell, Interstitial Cells, Testicular Interstitial Cells, Whole Transcriptome Shotgun Sequencing, Testicular Interstitial, Testicular Interstitial., RNA-seq, Dietary Fiber, Adults, Leydig, adult, Testicular, Cell, Testicular Interstitial Cell0.00.00.00.00.0falseBulk RNA-seq of fetal and adult murine Leydig cellsBulk RNA-seq of fetal and adult murine Leydig cells2022-05-122021-06-30PRJNA720700GSE1717463426290710090