{"database":"panorama","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Kuei-Ho Chen"],"species":["Homo Sapiens"],"full_dataset_link":["https://panoramaweb.org/9R0PpO.url"],"submitter_email":["kueiho.chen@gladstone.ucsf.edu"],"submitter_affiliation":["J. David Gladstone Institutes, San Francisco, CA"],"sample_protocol":[""],"repository":["PanoramaPublic"],"data_protocol":[""],"pubmed_abstract":["Mechanisms specifying cancer cell states and response to therapy are incompletely understood. Here we show epigenetic reprogramming shapes the cellular landscape of schwannomas, the most common tumors of the peripheral nervous system. We find schwannomas are comprised of 2 molecular groups that are distinguished by activation of neural crest or nerve injury pathways that specify tumor cell states and the architecture of the tumor immune microenvironment. Moreover, we find radiotherapy is sufficient for interconversion of neural crest schwannomas to immune-enriched schwannomas through epigenetic and metabolic reprogramming. To define mechanisms underlying schwannoma groups, we develop a technique for simultaneous interrogation of chromatin accessibility and gene expression coupled with genetic and therapeutic perturbations in single-nuclei. Our results elucidate a framework for understanding epigenetic drivers of tumor evolution and establish a paradigm of epigenetic and metabolic reprograming of cancer cells that shapes the immune microenvironment in response to radiotherapy."],"pubmed_title":["Epigenetic reprogramming shapes the cellular landscape of schwannoma."],"pubmed_authors":["Liu S John SJ, Casey-Clyde Tim T, Cho Nam Woo NW, Swinderman Jason J, Pekmezci Melike M, Dougherty Mark C MC, Foster Kyla K, Chen William C WC, Villanueva-Meyer Javier E JE, Swaney Danielle L DL, Vasudevan Harish N HN, Choudhury Abrar A, Pak Joanna J, Breshears Jonathan D JD, Lang Ursula E UE, Eaton Charlotte D CD, Hiam-Galvez Kamir J KJ, Stevenson Erica E, Chen Kuei-Ho KH, Lien Brian V BV, Wu David D, Braunstein Steve E SE, Sneed Penny K PK, Magill Stephen T ST, Lim Daniel D, McDermott Michael W MW, Berger Mitchel S MS, Perry Arie A, Krogan Nevan J NJ, Hansen Marlan R MR, Spitzer Matthew H MH, Gilbert Luke L, Theodosopoulos Philip V PV, Raleigh David R DR"],"additional_accession":[]},"is_claimable":false,"name":"Vestibular schwannoma is comprised of neural crest and immune subgroups","description":"Vestibular schwannomas (VS) are tumors arising from cranial nerve Schwann cells that cause significant morbidity and are treated with surgery and irradiation. We describe a DNA methylation-based classification of VS into neural crest and immune enriched subgroups and validate our findings using bulk and single cell transcriptomics. Neural crest enriched VS express primary cilia and are associated with misactivation of the Hedgehog pathway. Irradiation epigenetically reprograms VS cells to reduce ciliary length, attenuate Hedgehog signaling, and induce cellular stress mechanisms that recruit lymphocytes and macrophages, transforming recurrent tumors in pairs of patient-matched samples from neural crest to immune enriched VS. Using single cell transcriptomics, we develop a VS cell atlas and identify a diversity of schwannoma and non-schwannoma cell-types that resemble different stages of nerve injury and regeneration. These data elucidate the molecular pathways underlying VS and establish a framework for understanding how irradiation modulates the tumor microenvironment.","dates":{"publication":"Wed Jun 17 00:00:00 BST 2026"},"accession":"PXD014883","cross_references":{"TAXONOMY":["9606"],"pubmed":["38216587"]}}