panoramaProteomicsOlga ShevchukMus Musculushttps://panoramaweb.org/BnW2U9.urlolga.shevchuk@isas.deISAS e.V. Otto-Hahn-Straße 6b 44227 Dortmund, GermanyPanoramaPublicGrowth differentiation factor 11 (GDF11) is a TGF-β superfamily circulating factor that regulates cardiomyocyte size in rodents, sharing 90% amino acid sequence identity in the active domains with myostatin (GDF8)-the major determinant of skeletal muscle mass. Conflicting data on age-related changes in circulating levels have been reported mainly due to the lack of specific detection methods. More recently, liquid chromatography tandem mass spectrometry (LC-MS/MS) based assay showed that the circulating levels of GDF11 do not change significantly throughout human lifespan, but GDF8 levels decrease with aging in men. Here a novel detection method is demonstrated based on parallel reaction monitoring LC-MS/MS assay combined with immunoprecipitation to reliably distinguish GDF11 and GDF8 as well as determine their endogenous levels in mouse serum. The data indicate that both GDF11 and GDF8 circulating levels significantly decline with aging in female mice.Targeted Approach to Distinguish and Determine Absolute Levels of GDF8 and GDF11 in Mouse Serum.Camparini Luca L, Kollipara Laxmikanth L, Sinagra Gianfranco G, Loffredo Francesco S FS, Sickmann Albert A, Shevchuk Olga O, Shevchuk Olga OHeart, liquid chromatography tandem mass spectroscopy, Co-Immunoprecipitations, Heart Muscle Cells, determination, multiple endocrine neoplasia syndrome, Laboratory, Blood, postnatal development, Myocyte, Mus domesticus, growth and development, CASP-14, Aging, Serum, House Mouse, Co Immunoprecipitation, Cardiac Muscle, Techniques, DSmurf, Method, Biological, Cmpt, cardiomyocyte, Muscle Cell, Senescence, Life Span, LCMSMS, Man (Taxonomy), entire life cycle, Length of Life, Co-Immunoprecipitation, Cardiac Myocytes, Precipitation, Swiss Mice, procedures, Boys, Structures, Biological Aging, reaction, Immune, Methodological Studies, Cardiomyocytes, Smurf, scientific observation, ELL1, Ell1, GDF8, Gdf8, house mouse, Precipitations, GDF-8, CG4943, D-smurf, LC-MS2, Primary Protein Structure, Lifespans, Multiple endocrine neoplasms, BMP-11, DmelCG10120, MSLHP, rodents, Modern, LC-MS/MS, mouse, CASP14, Growth Differentiation Factor 8, Lack, Procedure, Blood Serum, cardiocyte, female, familial endocrine, Dipodidae, Hydrochaeri, RENBP, Mini-ICE, multiple endocrine adenomatosis, Amino Acid, Mus musculus, Caspase-14 subunit p10, growth pattern, DmelCG4943, non-developmental growth, mice, life, Swiss Mouse, Adenomatosis, Caspase-14 subunit p19, Methodological, Beaver, MICE, Methodological Study, human, AGE, domesticus, Heart Muscle Cell, Endocrine neoplasia, multiple, liquid chromatography-tandem mass spectroscopy, C19orf17, Rodentias, Cells, heart muscle cell, anon-WO0118547.278, Mouse, multiple endocrine neoplasia syndrome(s), Myostatin, Mdh-NADP, Amino Acid Sequences, 3.4.22.-, lifespan, human being, Procedures, dSmurf1, men syndromes, Capybara, Multiple endocrine neoplasia, mini-ICE, rodent, LC-MS-MS, Human, method, Cardiac, Homo sapiens, House, cardiac muscle fiber, method used in an experiment, LC-MSMS, Studies, Immune Precipitations, Mus musculus domesticus, Mice, Technique, Man, Immune Precipitation, muscle mass measurement, entire lifespan, Swiss, multiple endocrine neoplasia, Castor Beaver, Muscle Cells, Structure, Study, men syndrome, d-smurf, Protein Structure, Jerboas, muscle mass, Cardiac Myocyte, Primary Protein, Lifespan, Hydrochaeris, measuring, BMP11, Life Spans, female human body, RnBP, Multiple Endocrine Neoplasia, Bmp11, MDH, Heart Muscle, GlcNAc 2-epimerase, Primary, ME, Dsmurf, Cell, development, Sequences, dSmurf, N-acetyl-D-glucosamine 2-epimerase, LC/MS/MS, Mus, Sequence, cardiac muscle cell, Smurf ubiquitin ligase, chemical analysis, Familial endocrine adenomatosis, Cardiomyocyte, techniques, Protein Structures, MEN, Men, Me, Mus musculus., Beavers, postnatal growth, mem, House Mice, Rodents, Jerboa, Cardiac Muscle Cells, men, Cardiac Muscle Cell, Capybaras, Rodent, Laboratory Mice, plan specification, PPP1R68, Modern Man, cardiac myocyte, renin-binding protein, liquid chromatography tandem mass spectrometry, Multiple endocrine adenomatosis, assay, Primary Protein Structures, growth, Laboratory Mouse, Serums, Females, methodology, CG10120measuring, Mus musculus, BMP11, PP14, BMP-11, determination, gdf-5, Laboratory, Swiss, mice, Mus domesticus, Bmp11, mouse, Swiss Mouse, Blood., Growth Differentiation Factor 8, House Mice, Swiss Mice, Blood Serum, Serum, House Mouse, GD, domesticus, Laboratory Mice, GdA, GdF, gdf, concentration, House, Mus, scientific observation, chemical analysis, GDF8, Mus musculus domesticus, Mouse, assay, PAEG, house mouse, GdS, Myostatin, PEP, Mice, Laboratory Mouse, GDF-8, Serums, CDMP1Mus musculus, BMP11, BMP-11, Laboratory, Swiss, MSLHP, mice, Mus domesticus, Bmp11, mouse, Swiss Mouse, Blood., House Mice, Swiss Mice, Blood Serum, Serum, House Mouse, domesticus, Laboratory Mice, House, Mus, Cmpt, Gdf8, GDF8, Mus musculus domesticus, Mouse, house mouse, Mice, Laboratory Mouse, SerumsHeart, liquid chromatography tandem mass spectroscopy, FBgn0003149, Psychological Power, 3.4.22.-, Procedures, determination, Professional Power, Laboratory, postnatal development, Mus domesticus, Heart Hypertrophy, metazoans, Gene, mini-ICE, growth and development, CASP-14, Aging, animals, House Mouse, Enlargement, LC-MS-MS, CG11121, Techniques, method, Personal, Para, Cardiac, old, DmelCG11121, House, Method, mPM, method used in an experiment, Cmpt, LC-MSMS, Studies, Gene Products, Mus musculus domesticus, Animal, Heart Hypertrophies, CG5939, Mice, Technique, Animalia, Psychological, study, Senescence, somda, LCMSMS, Heart Enlargement, Swiss, Swiss Mice, procedures, SO, Social, Biological., Study, set, Biological Aging, Abstract, Methodological Studies, Enlarged Heart, grupos, scientific observation, GDF8, Gdf8, Social Power, GDF-8, So, Power, Drl, LC-MS2, measuring, BMP11, grupo, BMP-11, cardiomegaly, MSLHP, DmelCG5939, Bmp11, Proteins, LC-MS/MS, CASP14, mouse, Growth Differentiation Factor 8, myocardial hypertrophy, Psychological Powers, Procedure, Mdu, group, heart hypertrophy, development, LC/MS/MS, prm, Mus, mda, Metazoa, chemical analysis, Protein, Mini-ICE, l(3)S010605, Professional, Cardiac Hypertrophies, techniques, Hypertrophies, ami, Hypertrophy, Mus musculus, Caspase-14 subunit p10, growth pattern, ensemble, 0106/05, non-developmental growth, med, mice, PM/mPM, postnatal growth, Swiss Mouse, Rest, House Mice, Caspase-14 subunit p19, Methodological, MICE, Methodological Study, Cardiac Hypertrophy, Laboratory Mice, domesticus, plan specification, Protein Gene Products, multicellular animals, Gene Proteins, Enlarged, Personal Power, l(3)10631, liquid chromatography-tandem mass spectroscopy, Power (Psychology), liquid chromatography tandem mass spectrometry, Mouse, enlarged heart, assay, Myostatin, Powers, growth, groupe, Laboratory Mouse, Elderly, pm, PM, methodology, GruppefalseTargeted assay for differentiation and monitoring the concentration of GDF 8 (Myostatin) and GDF11 in mouse serumAbstract Growth differentiation factor 11 (GDF11) and Myostatin (GDF8) are circulating factor shown to reduce cardiac hypertrophy of aged animals. Although GDF11 and GDF8 have high structural homology, it seems that GDF11 is had a more potent effect on reducing cardiomyocytes size, albeit GDF11 quantification methods have been recently questioned. In this study, we developed the IP based-PRM- LC-MS/MS method for measuring the GDF8 and GDF11 in one assay. The method demonstrated discriminatory power and allow the quantification of both proteins. Comparison of young and old group of mice show declination of both, GDF8 and GDF11 during aging.Sun Jan 24 00:00:00 GMT 2021PXD0158381009032104967