panorama0000ProteomicsSanjeeva SrivastavaHomo SapiensSevere Acute Respiratory Syndrome Coronavirus 2https://panoramaweb.org/COVID_Swab_MRM.urlsanjeeva@iitb.ac.inIIT BOMBAYPanoramaPublicThe altered molecular proteins and pathways in response to COVID-19 infection are still unclear. Here, we performed a comprehensive proteomics-based investigation of nasopharyngeal swab samples from patients with COVID-19 to study the host response by employing simple extraction strategies. Few of the host proteins such as interleukin-6, L-lactate dehydrogenase, C-reactive protein, Ferritin, and aspartate aminotransferase were found to be upregulated only in COVID-19-positive patients using targeted multiple reaction monitoring studies. The most important pathways identified by enrichment analysis were neutrophil degranulation, interleukin-12 signaling pathways, and mRNA translation of proteins thus providing the detailed investigation of host response in COVID-19 infection. Thus, we conclude that mass spectrometry-detected host proteins have a potential for disease severity progression; however, suitable validation strategies should be deployed for the clinical translation. Furthermore, the <i>in silico</i> docking of potential drugs with host proteins involved in the interleukin-12 signaling pathway might aid in COVID-19 therapeutic interventions.Proteomic investigation reveals dominant alterations of neutrophil degranulation and mRNA translation pathways in patients with COVID-19.Bankar Renuka R, Suvarna Kruthi K, Ghantasala Saicharan S, Banerjee Arghya A, Biswas Deeptarup D, Choudhury Manisha M, Palanivel Viswanthram V, Salkar Akanksha A, Verma Ayushi A, Singh Avinash A, Mukherjee Amrita A, Pai Medha Gayathri J MGJ, Roy Jyotirmoy J, Srivastava Alisha A, Badaya Apoorva A, Agrawal Sachee S, Shrivastav Om O, Shastri Jayanthi J, Srivastava Sanjeeva SCOVID19, host organism, determination, COVID-19, Infestations and Infections, Virus Infection, SARS-CoV infection, COVID-19 Virus Diseases, 2019 novel coronavirus, SARS ASSOC CORONAVIRUS, Virus Disease, 2019 nCoV Disease, School-Age, Polypeptides, serRS, peptido, SARS, 2019-nCoV Disease, SARS CoV 2 Infection, 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Drug, Gene Proteins, Preparations, L-Lactate, Dipotassium, Mg-5-Longoral, B Cell Differentiation Factor, Ammonium Aspartate, Hybridoma, signalling process, Lactate Dehydrogenase, Therapeutic, B-Cell Stimulatory Factor 2, Treatment, assay, response, fthl6, Sodium Aspartate, IL-12 p70, PTX1, Monosodium Aspartate, Polyadenylated mRNA, Pharmaceutical Products, COVID 19, Calcium Salt, L-Aspartate, Pharmaceutical Preparation0falseValidation of detected viral and host peptides from COVID-19 nasopharyngeal swab samplesIn the current scenario of COVID-19, an assay that can efficiently and specifically detect infection by SARS-COV2 is required. Nasopharyngeal swabs are non-invasive samples and can be conveniently acquired from the population to be screened. In this work, we have validated a few viral and host peptides (found to be relevant from our shotgun data) using a Triple Quadrupole mass spectrometer.Fri Apr 02 00:00:00 BST 2021PXD0218452697049960633558857