{"database":"panorama","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Sanjeeva Srivastava"],"species":["Homo Sapiens"],"full_dataset_link":["https://panoramaweb.org/longitudinal_plasma_MRM.url"],"submitter_email":["sanjeeva@iitb.ac.in"],"submitter_affiliation":["Biosciences and Bioengineering Department, IIT Bombay"],"sample_protocol":[""],"repository":["PanoramaPublic"],"data_protocol":[""],"pubmed_abstract":["Severe coronavirus disease 2019 (COVID-19) infection may lead to lung injury, multi-organ failure, and eventually death. Cytokine storm due to excess cytokine production has been associated with fatality in severe infections. However, the specific molecular signatures associated with the elevated immune response are yet to be elucidated. We performed a mass-spectrometry-based proteomic and metabolomic analysis of COVID-19 plasma samples collected at two time points. Using Orbitrap Fusion LC-MS/MS-based label-free proteomic analysis, we identified around 10 significant proteins, 32 significant peptides, and 5 metabolites that were dysregulated at the severe time points. Few of these proteins identified by quantitative proteomics were validated using the multiple reaction monitoring (MRM) assay. Integrated pathway analysis using distinct proteomic and metabolomic signatures revealed alterations in complement and coagulation cascade, platelet aggregation, myeloid leukocyte activation pathway, and arginine metabolism. Further, we highlight the role of leukocyte activation and arginine metabolism in COVID-19 pathogenesis and targeting these pathways for COVID-19 therapeutics."],"pubmed_title":["A Multi-omics Longitudinal Study Reveals Alteration of the Leukocyte Activation Pathway in COVID-19 Patients."],"pubmed_authors":["Suvarna Kruthi K, Salkar Akanksha A, Palanivel Viswanthram V, Bankar Renuka R, Banerjee Nirjhar N, Gayathri J Pai Medha M, Srivastava Alisha A, Singh Avinash A, Khatri Harsh H, Agrawal Sachee S, Shrivastav Om O, Shastri Jayanthi J, Srivastava Sanjeeva S"],"additional_accession":[]},"is_claimable":false,"name":"Validation of proteins showing significant dysregulation from shotgun proteomics experiments in longitudinal plasma samples of COVID-19 patients","description":"A Label Free Quantification study was done to observe the changes in the plasma proteome of patient samples collected at two different time points of disease severity (T1 and T2). A few of the proteins that were significantly dysregulated between the two time points were taken forward for validation using the Multiple Reaction Monitoring approach. The Skyline file here shows two proteins: 10 Vitamin D binding protein and Profilin 1.","dates":{"publication":"Mon Jun 27 00:00:00 BST 2022"},"accession":"PXD026848","cross_references":{"TAXONOMY":["9606"],"pubmed":["34379420"]}}