panorama0000ProteomicsSanjeeva SrivastavaSRM/MRMHomo Sapienshttps://panoramaweb.org/covid19semen.urlsanjeeva@iitb.ac.inBiosciences and Bioengineering Department, IIT BombayPanoramaPublicA considerable section of males suffered from COVID-19, with many experiencing long-term repercussions. Recovered males have been documented to have compromised fertility, albeit the mechanisms remain unclear. We investigated the impact of COVID-19 on semen proteome following complete clinical recovery using mass spectrometry. A label-free quantitative proteomics study involved 10 healthy fertile subjects and 17 COVID-19-recovered men. With 1% false discovery rate and >1 unique peptide stringency, MaxQuant analysis found 1099 proteins and 8503 peptides. Of the 48 differentially expressed proteins between the healthy and COVID-19-recovered groups, 21 proteins were downregulated and 27 were upregulated in COVID-19-recovered males. The major pathways involved in reproductive functions, such as sperm-oocyte recognition, testosterone response, cell motility regulation, adhesion regulation, extracellular matrix adhesion, and endopeptidase activity, were downregulated in COVID-19-recovered patients according to bioinformatics analysis. Furthermore, the targeted approach revealed significant downregulation of semenogelin 1 and prosaposin, two proteins related to male fertility. Therefore, we demonstrate the alteration of semen proteome in response to COVID-19, thus disrupting the male reproductive function despite the patient's clinical remission. Hence, to understand fertility-related biological processes triggered by this infection, a protracted evaluation of the consequences of COVID-19 in recovered men is warranted.Semen Proteomics of COVID-19 Convalescent Men Reveals Disruption of Key Biological Pathways Relevant to Male Reproductive Function.Ghosh Susmita S, Parikh Swapneil S, Nissa Mehar Un MU, Acharjee Arup A, Singh Avinash A, Patwa Dhruv D, Makwana Prashant P, Athalye Arundhati A, Barpanda Abhilash A, Laloraya Malini M, Srivastava Sanjeeva S, Parikh Firuza FCOVID19, other disease, testicle, Effects, COVID-19, number, Virus Infection, COVID-19 Virus Diseases, 2019 novel coronavirus, Spectrum Analyses, Ximpact, Virus Disease, presence, 2019 nCoV Disease, Long Term, element, protrusion, gonad of male reproductive system, diseases, 2019-nCoV Disease, SARS CoV 2 Infection, gonad of male genitalia, Mass, Severe Acute Respiratory Syndrome Coronavirus 2 Infection, disease or disorder, diseases and disorders, β-coronavirus, 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Sustanon, Social Control, Down-Regulation (Physiology), endoprotease activity, chemical analysis, Protein, Familial endocrine adenomatosis, Phenomena, Infection, Natural Fertility, IMPACT, imprinted and ancient gene protein homolog, Men, MEN, Sterotate, Me, Determinants, Y-Bearing, Mass Spectrum Analyses, CG10120., Plasma, Mass Spectrum, betacoronavirus, Disease 2019, CG4216, mem, patient, men, COVID-19 Pandemics, COVID-19 Pandemic, SARS-coronavirus 2, Protein Gene Products, male human body, Gene Proteins, Differential, Y-Bearing Sperms, PPP1R68, Marital, 17-beta-Hydroxy-4-Androsten-3-one, Multiple endocrine adenomatosis, Peptid, regulation, proteinase, assay, Receptor, Seminal, COVID 19, RWDD5, Fertility Survey, CG10120, 8 Isotestosterone0falseTargeted validation of significantly altered semen proteins in COVID-19 patientsThe current global outbreak of COVID-19 has significantly impacted several organ systems. Male reproductive organs are among the potential targets of SARS-CoV-2 infection owing to the presence of abundant viral receptors; ACE2 and TMPRSS2 in the testis. However, the questions pertaining to the long term effects of SARS-CoV-2 infection on male fertility are still unanswered. For this study, we procured the semen samples from COVID-19 recovered patients and performed mass spectrometry based proteomic studies to understand the impact of the disease on the biological processes involved in reproductive health.Sun Jun 26 00:00:00 GMT+01:00 2022PXD026849960635309488