panoramaProteomicsMehar Un NissaSRM/MRMLabeo Rohitahttps://panoramaweb.org/rohuliverproteomicsah.urlkohlimeharunnissa@gmail.comBiosciences and Bioengineering Department, IIT BombayPanoramaPublicAeromonas hydrophila (Ah) is a Gram-negative bacterium and a serious global pathogen causing Motile Aeromonas Septicaemia (MAS) in fish leading to global loss in aquaculture. Investigation of the molecular alterations of host tissues such as liver could be a powerful approach to identify mechanistic and diagnostic immune signatures of disease pathogenesis. We performed a proteomic analysis of Labeo rohita liver tissue to examine the protein dynamics in the host cells during Ah infection. The proteomic data was acquired using two strategies; discovery and targeted proteomics. Label-free quantification was performed between Control and challenged group (AH) to identify the differentially expressed proteins (DEPs). A total of 2525 proteins were identified and 157 were DEPs. DEPs include metabolic enzymes (CS, SUCLG2), antioxidative proteins, cytoskeletal proteins and immune related proteins (TLR3, CLEC4E). Pathways like lysosome pathway, apoptosis, metabolism of xenobiotics by cytochrome P450 were enriched by downregulated proteins. However, upregulated proteins majorly mapped to innate immune system, signaling of B cell receptor, proteosome pathway, ribosome, carbon metabolism and protein processing in ER. Our study would help in exploring the role of Toll-like receptors, C-type lectins and, metabolic intermediates like citrate and succinate in Ah pathogenesis to understand the Ah infection in fish. SIGNIFICANCE: Bacterial diseases such as motile aeromonas septicaemia (MAS) are among the most serious problems in aquaculture industry. Small molecules that target the metabolism of the host have recently emerged as potential treatment possibilities in infectious diseases. However, the ability to develop new therapies is hampered due to lack of knowledge about pathogenesis mechanisms and host-pathogen interactions. We examined alterations in the host proteome during MAS caused by Aeromonas hydrophila (Ah) infection, in Labeo rohita liver tissue to find cellular proteins and processes affected by Ah infection. Upregulated proteins belong to innate immune system, signaling of B cell receptor, proteosome pathway, ribosome, carbon metabolism and protein processing. Our work is an important step towards leveraging host metabolism in targeting the disease by providing a bigger picture on proteome pathology correlation during Ah infection.Proteomic analysis of liver tissue reveals Aeromonas hydrophila infection mediated modulation of host metabolic pathways in Labeo rohita.Nissa Mehar Un MU, Pinto Nevil N, Ghosh Biplab B, Singh Urvi U, Goswami Mukunda M, Srivastava Sanjeeva Sjecur, Metabolic Networks, Networks, Livers, host organism, Pathway, Cyprinus rohita, Jayanti rohu., Metabolic, proteomic analysis, Aeromonas hydrophila disease or disorder, Tissue, Metabolic Pathway, iecur, simple tissue, Pathways, Network, Aeromonas hydrophila caused disease or disorder, Metabolic Network, Aeromonas hydrophila infectious disease, Metabolic PathwaysProtein Gene Products, jecur, Gene Proteins, Livers, Cyprinus rohita, Aeromonas hydrophila disease or disorder., Protein, Gene Products, Proteins, Tissue, iecur, Gene, simple tissue, Aeromonas hydrophila caused disease or disorder, Jayanti rohu, Aeromonas hydrophila infectious diseaseB Cells, F14M4.21, l(3)LG9, DL3735W, F6G17_50, F14M4.22, MJB24.3, POLYPEPTIDE 7, FAMILY 77, MJB24.7, Tertiary Sectors, Metabolic Concepts, B cell, A4, ribosomal RNA, T20B5_9, Cytochrome P 450 Monooxygenase, F4P12.1, C230050L11, Carbon-12, F6G17_60, Aeromonas liquefaciens, T11J7.16, TSK5, T11J7.14, F3L17.70, Grp1, 1, 2, Metabolism Concept, MOJ9.6, Infectious Diseases, F6G17.50, T11J7_16, T11J7_14, gamma sarcoglycan, C, Toll Like Receptor, molecules, DM10, stimulation by symbiont of host programmed cell death, Cytoskeletal, CYP90A, Tissue, F2P9_2, MDN11.4, DL3710C, F6G17.60, F3L17_70, Toll Like Receptors, Host Pathogen Interactions, citrate, F6A4.110, g, F22H5.19, F6G17_80, Homo sapiens disease, CYTOCHROME P450, CG4943, F13O11.20, F6A4.120, AI957183, anatomical protrusion, Infectious Disease, DM33, DM36, F13O11.25, F13O11.23, F13O11.24, T29H11.210, THALIAN-DIOL DESATURASE, CYP450 Family, not genetically inherited, cytochrome P450 activity, F6G17.80, DWF3, signaling (initiator) caspase activity, Role Concept, induction of apoptosis, meconium aspiration, DM23, secretion, simple tissue, DL3600C, CYP703, F11C18.7, 4 Butanedioic Acid, F11C18.9, F5G3_8, CG11628, Communicable, Host-Pathogen, cit, Infection and Infestation, F5M6.19, Sector, T5J17.120, B-Lymphocyte, 35kD dystrophin-associated glycoprotein, F13O11_20, Extrinsic Pathway, T29H11.200, F5M6_19, F13O11_24, F13O11_25, F13O11_23, F6A4.160, Lectin, DWARF 3, GRP1/cytohesin 1, Peptidomics, fishes, regulation by symbiont of host system process, AF171077, F6A4.170, Agnatha, jecur, CG11633, DM63, Host-Pathogen Relations, Cytochrome P-450 Families, Gene Products, T12H17_100, CG5893, MAM, MAS, succinates, F5A8_3, syndrome, clinical infection, T29A15_200, F21J6.102, CD283, Enzyme, T8K22_12, F21J6.4, F21J6.2, 3-propanetricarboxylate, F13D4_120, K15C23.6, AI256723, grupo, polypeptide 25, polypeptide 26, hemolysin activity, degradation, polypeptide 23, polypeptide 24, ATR4, polypeptide 21, SH3MD1, polypeptide 22, polypeptide 20, Sox70, disorders, polypeptide 18, polypeptide 19, polypeptide 16, polypeptide 17, Dsmurf, T8K22.12, carbone, Concept, carbono, dSmurf, F5I10.21, xenobiotic compounds, Tertiary, native protein, polypeptide 14, polypeptide 15, polypeptide 12, polypeptide 13, polypeptide 10, Pathogen-Host Interactions, polypeptide 11, Smurf ubiquitin ligase, proteomic analysis, family 71 subfamily B, F3F19_13, condition, Lectins, metabolism, THAD1, P-450 Enzyme, F3F19.10, F5I10_21, F3F19.11, F3F19.12, F3F19.13, free ribosome, Ribosome, RED1, ensemble, ATT1, Bursa-Equivalent Lymphocyte, polypeptide 38, ion(2-), polypeptide 36, signalling process, polypeptide 37, polypeptide 34, Host, cytochrome P450, polypeptide 35, polypeptide 32, polypeptide 33, polypeptide 30, polypeptide 31, microtubule organising centre attachment site, polypeptide 29, polypeptide 27, polypeptide 28, CYTOCHROME P450 MONOOXYGENASE 83B1, 2-Ethanedicarboxylic Acid, Interactions, Anabolism, biochemical pathways, F18A5_160, Metabolic Process, Immune Systems, Bursa-Dependent Lymphocytes, Infestations and Infections, ion(3-), protein, neutral molecular compounds, Cytochrome P 450, MXI10.18, Pathogen-Host Interaction, DSmurf, T9E8.50, MOJ9_6, F3O9_20, Roles, Pathogen Host Interactions, Monooxygenase, pathogenesis, MXI10_18, T9E8_50, protein aggregate, Phenomenon, REF2, prevention and control, F3O9.20, molecule, Host-Pathogen Relation, F13P17.35, molecula, human disease, UNFERTILIZED EMBRYO SAC 9, SUPERROOT 2, YUP8H12.23, F3F19_10, F25C20_25, F25C20_24, F3F19_12, F3F19_11, hypoplasia, iecur, Fish, CYP81F1, F13P17.22, F25C20.25, F25C20.24, Immune, B lymphocyte, l(2)k08110, disease management, biotransformation, YUP8H12_23, MJB24_3, AT5G57250, MJB24_7, D-smurf, Cytochrome P 450 Enzyme System, F6A4_110, Cytochrome P450 Superfamily, Caspase-Dependent Apoptosis, Isolectins, Communicable Diseases, Citric Acid Monohydrate, P 450 Enzyme, 2-hydroxytricarballylate, Jayanti rohu, Autolysosomes, F6A4_120, T22C12.3, ray-finned fishes, Aeromonas dourgesi, P-450, P450 Enzyme, CYTOCHROME P450 90A1, Monorhina, histopathology, Step, F12B17_50, System, F12B17.40, Treatments, Cytochrome p450 Families, TKS5, Host-Pathogen Interaction, 2-hydroxy-1, disease, Vitreous Carbon, T9E8.30, DMDA, F12B17_40, T9E8_30, Apoptoses, CYTOCHROME P450 MONOOXYGENASE 81F1, Gruppe, Ammonium, Bioflocs, Lysosome, SGCG_HUMAN, Cytochrome P-450 Oxygenase, caspase-dependent programmed cell death, CABBAGE 3, KLU, carbon, cit(3-), loD, cytohesin/GRP1, Relations, Metabolism, F13D4.120, Toll Like, tiny, F6A4_160, Animal Lectin, study, Cytochrome P-450, IIAE2, F11C18.12, l(2)SH2 0323, F6A4_170, meconium, Infestation and Infection, Neural-specific protein-tyrosine phosphatase, Cytochrome P 450 Enzyme, D6Wsu120e, causality, CONSTITUTIVE PHOTOMORPHOGENIC DWARF, disorder, F6F3.25, Controlled, small, Controlling, activation by organism of non-apoptotic programmed cell death in other organism, F21J6_102, succinic acid anion, protein complex, Infectious, CYP450, enzyme activity, medical condition, F6G17.1, F6G17.6, F6G17.5, F6G17.8, group, Enzymes, Metabolism Concepts, CYP90A1, SOX70D, Programmed Cell Death, Infection, butanedioic acid, membrane bound ribosome, reactive hemophagocytic lymphohistiocytosis, CYH1, biodegradation, Metabolic, F1C12.160, Cytochrome P 450 Superfamily, Carbon 12, Protein Gene Products, F8N16.14, F8N16.15, Cytochrome P-450-Dependent, Therapeutic, SCARMD2, Animal Lectins, Treatment, F8N16_14, Pathogen Host Interaction, F8N16_15, Cytochrome P 450 Families, K8K14.3, aspiration syndrome, A_IG005I10.21, MTOC attachment site, Receptors, A_IG005I10_21, prevention, MFB13_19, SUR2, Cytochrome P 450 Oxygenase, F14P1_4, GRP1, CYP77A7, Bacterium hydrophilum, Ammonium Succinate, succinate anion, treatment, bony fishes, AW556404, K24M7_14, MJJ3.9, catabolism, ABERRANT INDUCTION OF TYPE THREE 1, PTPSTEP, MFB13.19, metabolic process resulting in cell growth, CYTOCHROME P450 79F2, Molekuel, K24M7.14, P450 protein, T1E3_20, set, Intrinsic Pathway Apoptosis, Cytochrome P450, Smurf, T1E3.20, Role Concepts, Infections and Infestations, single organism signaling, GPH, preventive therapy, Citrate, B Lymphocytes, metabolism resulting in cell growth, Toll-Like Receptor, T1E3.4, Potassium Succinate, Lack, Cytochrome P-450 Superfamily, peptidolysis during protein maturation, CITRATE ANION, Vitreous, Interaction, Cephalaspidomorphi, DL3720W, Role, sarcoglycan, aspiration, AI413738, Caspase-Dependent, T22C12_3, clinical infection., CYP89, Anhydrous Citric Acid, Cytochrome P 450 Enzymes, CBB3, T26C19.1, Relation, protein maturation by proteolysis, Striatum-enriched protein-tyrosine phosphatase, MFD22.6, Intrinsic Pathway Apoptoses, LUTEIN DEFICIENT 5, CYP90, Aeromonas hydrophila (Chester 1901) Stanier 1943 (Approved Lists 1980), meconium inhalation, activation by symbiont of host programmed cell death, Cytochrome P-450 Enzyme, K9E15_12, T4C9.140, T12H17.80, Sectors, Biocatalysts, Processes, PFD, 2310014D11Rik, K9E15.12, Metabolic Processes, protein-containing complex, F1C12_160, induction by organism of non-apoptotic programmed cell death in other organism during symbiotic interaction, K16E1.5, K16E1.6, Host Pathogen Interaction, SCS-betaG, disease or disorder, T12H17_80, YUP8H12R.1, RED ELONGATED 1, gamma-SG, F12B17.50, Industries, P-450 Enzymes, T4C9.160, Butanedioic Acid, Metabolic Concept, T4C9.150, SUBFAMILY A, F6F3.8, Classic Apoptosis, Host Pathogen, Isolectin, Anhydrous, Potassium, Apoptosis, DmelCG5893, ALTERED TRYPTOPHAN REGULATION 4, Sox B2-1, F17K2.9, F17K2.8, LUT5, 35 kDa dystrophin-associated glycoprotein, K8K14_3, Proteins, stepk, CYTOCHROME P450 MONOOXYGENASE 71B2, Cell, Xenobiotic, family 735, SGCG, Actinopterygi, subfamily K, subfamily H, subfamily F, subfamily G, RUNT 1, T4C9.170, subfamily D, subfamily B, F3M18.13, subfamily C, subfamily A, P450 Enzymes, CYTOCHROME P450 MONOOXYGENASE, THAD, polypeptide 30 pseudogene, KAO1, T3A4.4, Metabolic Phenomenon, RNT1, F1M20_23, multicellular organism metabolic process, Toll-Like, apoptosis, DMDA1, underdeveloped, prophylaxis, 3-propanetricarboxylic acid, DmelCG11628, Classic Apoptoses, Gene Proteins, SOX70, Superfamily, T20P8.6, F18A5.160, T20P8.5, control, T18B16_200, F1M20.23, commitment to apoptosis, F1M20.22, Receptor, (-)OOC-CH2-CH2-COO(-), biopsy, F1M20_22, MGC130048, host organism, Pathogen Interaction, Cyprinus rohita, T12C24_27, F7D19.15, Host Pathogen Relations, Sry-rDM33, positive regulation by symbiont of host non-apoptotic programmed cell death, Sry-rDM36, LFQ, Autolysosome, REDUCED EPIDERMAL FLUORESCENCE 2, polypeptide 2, polypeptide 1, ENT-KAURENOIC ACID OXYDASE 1, Succinate, polypeptide 8, F7D19_15, diseases, Extrinsic Pathway Apoptoses, polypeptide 7, polypeptide 9, polypeptide 4, polypeptide 3, polypeptide 6, polypeptide 5, Sry-rDM23, Concepts, diseases and disorders, cytopathology, CYTOCHROME P450 MONOOXYGENASE 91A2, Pathogen Interactions, family 703, Gm5098, F2P9.2, family 707, family 706, family 705, F14P1.4, family 709, MDN11_4, T9I4.17, reference sample, enzymes, 2 Ethanedicarboxylic Acid, 4-Butanedioic Acid, Pseudomonas hydrophila, F3M18_13, family 710, MJJ3_9, family 715, family 714, Sry-rDM10, family 712, preventive measures, family 718, T24P15_16, FCAALL.274, Classic, Carbon, Classical Apoptosis, FCAALL.270, Pathologies, T20B5.9, T12C24.27, Therapies, gamma-sarcoglycan, fish, T24P15.16, FISH, family 722, T9I4_17, family 721, Sox70D, Catabolism, Therapy, Process, polyostotic fibrous dysplasia, SG-gamma, Bacillus hydrophilus fuscus, CYP, Citric Acid, Communicable Disease, Cytochrome P-450 Enzymes, Intrinsic Pathway, Extrinsic Pathway Apoptosis, Diseases, F21F23_15, T5J17_120, 3.1.3.48, modulation by symbiont of host system process, GBETA, T20D16.18, T20D16.15, F5G3.8, T20D16_15, Sry-rDM63, FCAALL.208, F21F23.15, T20D16_18, Epistemology, DmelCG4943, 6C, succinate, Proteus hydrophilus, Biofloc, POFD, K16E1_6, gamma (35kDa dystrophin-associated glycoprotein), K16E1_5, Tks5, induction of apoptosis by p53, 3-propanetricarboxylate(3-), spine, STEP, carbonium, Biocatalyst, Classical, Proteomes, Cytochrome, other disease, SOXB2.1, F17K2.10, F17K2.11, gbeta, dSmurf1, Gene, Caspase Dependent Apoptosis, TYPE, protrusion, DAGA4, AA589508, Sh3md1, MYH9.18, Cytochrome P 450 Dependent Monooxygenase, reduced, T18B16.200, T4C9_140, 35DAG, Animal, Type I, F5A8.3, MYH9_18, Metabolism Phenomena, SCG3, Proteus ichthyosmius, B-cell, T29H11.190, F14M2.15, causes, F14M2.14, apoptosis activator activity, F6F3_8, P450, EG329070, non-neoplastic, F14M2_14, F14M2_15, CYP450 Superfamily, Kohlenstoff, d-smurf, grupos, K15C23_6, Disease, T4C9_170, FCAALL.280, P 450 Enzymes, total expressed protein, FCAALL.277, T20P8_6, KLUH, T20P8_5, Cytochrome P-450-Dependent Monooxygenase, LGMD2C, Metabolic Phenomena, family 90, T3A4_4, family 93, family 91, F6G17.10, macrophage activation syndrome, family 98, family 97, Protein, Systems, Phenomena, F1C12.160B, F1C12.160A, F6G17_10, l(2)SH0323, SOXDP, F11F12_12, Hyperotreti, T29A15.200, Uralyt U, family 83, family 82, family 81, Cytochrome P-450 Monooxygenase, family 87, DL3725W, family 86, T4C9_150, Pathogen-Host, family 89, family 88, Rest, F6G17_20, F6G17.20, YUP8H12R_1, Aquacultures, Osteichthyes, T21P5.11, Livers, family 72, family 71, ATKAO1, T4C9_160, family 76, family 79, family 78, family 77, F11F12.12, F11F12.13, Tertiary Sector, B-lymphocyte, groupe, T12H17.100, F11F12_13, T21P5_11aspiration syndrome, other disease, Bioflocs, SOXB2.1, Cyprinus rohita, l(3)LG9, MTOC attachment site, Peptidomics, SRML1, fishes, PFD, 2310014D11Rik, Sry-rDM33, Sry-rDM36, Infestations and Infections, Gene, protein, protein-containing complex, prevention, Agnatha, loD, protrusion, jecur, AA589508, DM63, Sh3md1, diseases, C230050L11, Aeromonas liquefaciens, TSK5, Gene Products, Sry-rDM23, disease or disorder, diseases and disorders, Bacterium hydrophilum, CG5893, protein aggregate, prevention and control, MAS, Proteus ichthyosmius, Gm5098, study, bony fishes, human disease, DM10, reference sample, MRM, Tissue, iecur, syndrome, Pseudomonas hydrophila, Fish, clinical infection, EG329070, Sry-rDM10, non-neoplastic, preventive measures, meconium, Infestation and Infection, set, g, grupos, disorder, Homo sapiens disease, Infections and Infestations, species, fish, FISH, Sox70D, Controlled, DmelCG5893, AI256723, Sox B2-1, Controlling, SPS1., preventive therapy, anatomical protrusion, grupo, DM33, DM36, protein complex, SH3MD1, Sox70, Proteins, PAPT, polyostotic fibrous dysplasia, disorders, medical condition, Bacillus hydrophilus fuscus, Jayanti rohu, SPDSY, group, Multiple Reaction Monitoring, Actinopterygi, macrophage activation syndrome, native protein, meconium aspiration, ray-finned fishes, Aeromonas dourgesi, DM23, SOX70D, Cephalaspidomorphi, Protein, vertebrates, Infection, Vertebrata, condition, simple tissue, Vertebrate, aspiration, AI413738, reactive hemophagocytic lymphohistiocytosis, Sry-rDM63, SOXDP, Hyperotreti, Monorhina, ensemble, prophylaxis, Rest, Proteus hydrophilus, Infection and Infestation, Biofloc, POFD, TKS5, Tks5, Aquacultures, Osteichthyes, Protein Gene Products, Gene Proteins, Livers, SOX70, disease, spine, control, Aeromonas hydrophila (Chester 1901) Stanier 1943 (Approved Lists 1980), meconium inhalation, microtubule organising centre attachment site, groupe, GruppefalseTargeted proteomic validation of dysregulated proteins in liver tissue of Labeo rohita after Aeromonas hydrophila infectionAeromonas hydrophila (Ah) is an opportunistic Gram-negative bacterium and a serious global pathogen causing Motile Aeromonas Septicaemia (MAS) in fish and many other vertebrates. This study aimed at identifying the cellular proteins and processes altered by Ah infection in liver tissue of Labeo rohita, an important aquaculture species. Discovery based proteomics data could identify a panel of differentially expressed proteins (DEPs) between Control and AH condition (Ah infected group). Protein abundance changes in a few selected DEPs was then validated using Selected reaction monitoring (SRM) approach.Tue May 02 00:00:00 GMT+01:00 2023PXD0294838464536906258