{"database":"panorama","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Robert Moulder"],"species":["Homo Sapiens"],"full_dataset_link":["https://panoramaweb.org/jbML1x.url"],"submitter_email":["robmou@utu.fi"],"submitter_affiliation":["Turku Bioscience"],"sample_protocol":[""],"repository":["PanoramaPublic"],"data_protocol":[""],"pubmed_abstract":["Regulatory T cells (Treg) play a central role in maintaining immune homeostasis, and the implementation of in vitro induced Treg cells (iTreg) to control immune function has significant potential in clinical medicine. The clinical application of iTreg has been limited by their poor stability. To better define the molecular characteristics of human iTreg, we performed a data-independent acquisition proteomics, detecting over 8000 proteins and providing a quantitative comparison of their relative levels in iTreg and activated Th0 cells. Consistent with the known molecular characteristics of Treg, several Treg signature proteins, including FOXP3, IKZF4, IL2RA, CTLA4, PD-1, IKZF3, LAG3, RUNX1 and hypermethylated in cancer 1, were identified and validated using Tier two targeted SRM and/or qRT-PCR. Notably, Leupaxin level was upregulated during Treg cell differentiation. Functional studies demonstrated that LPXN-deficient cells showed impaired expression of Treg protein markers FOXP3, IKZF4 and IKZF3 and impaired suppression of effector T cells. In addition, we identified a distinct CD160<sup>+</sup> iTreg subpopulation characterized by a distinct proteomic signature as compared to CD160<sup>-</sup> iTreg. Together, these findings provide a high-resolution proteomic landscape of human iTreg and identified a novel role of LPXN in the development and suppressive activity of iTreg."],"pubmed_title":["Proteomic Insights Into Human Induced Regulatory T Cells: The Role of LPXN and CD160 in Immune Suppression."],"pubmed_authors":["Batkulwar Kedar K, Andrabi Syed Bilal Ahmad SBA, Kattelus Roosa R, Buchacher Tanja T, Arnkil Ilona I, Starskaia Inna I, Rasool Omid O, Moulder Robert R, Lahesmaa Riitta R"],"additional_accession":[]},"is_claimable":false,"name":"Proteomics reveals the essential role for LPXN in human iTreg suppression","description":"Regulatory T cells are critical in the modulation of immune responses. Accordingly, the manipulation and implementation of in vitro induced Treg cells to control immune function has a significant potential in clinical medicine. However, the use of iTregs as therapeutics is complicated by their poor stability and variable suppressive activity. To gain a better understanding of the molecular characteristics of human iTreg cells, we conducted a data-independent analysis-proteomics, (detecting over 8000 proteins and providing quantitative comparison of their relative amounts in iTreg and activated Th0 cells). Consistent with the known molecular characteristics of Tregs, a full complement of Treg signature proteins, including FOXP3, EOS, IL2RA, CTLA4, PDCD1, IKZF3, LAG3, RUNX1 and HIC1were identified and validated using targeted proteomics and/or TaqMan assay. In addition, elevated levels of LPXN were observed during Treg cell differentiation. As a follow to the latter finding, we observed that LPXN deficient cells showed impaired expression of Treg protein markers, FOXP3, EOS, and IKZF3 and reduced ability to suppress effector cells. Collectively, this study characterized the proteomic signature of human iTregs and identified a novel role of LPXN in the development and suppressive activity of Tregs.","dates":{"publication":"Thu Aug 27 00:00:00 GMT+01:00 2026"},"accession":"PXD052690","cross_references":{"TAXONOMY":["9606"],"pubmed":["42624209"]}}