{"database":"panorama","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Philip Remes"],"species":["Homo Sapiens","Gallus Gallus","Escherichia Coli"],"full_dataset_link":["https://panoramaweb.org/stellar_ms_platform.url"],"submitter_email":["philip.remes@thermofisher.com"],"submitter_affiliation":["Thermo Fisher Scientific"],"sample_protocol":[""],"repository":["PanoramaPublic"],"data_protocol":[""],"description_synonyms":["FBgn0003149, IPP2A2, CDF, determination, Dint-1, DmelCG4889, BDSD, Blood, l(2)wg, Visible Light, 5730420M11Rik, Polypeptides, Techniques, Para, GAS-6, POF, peptido, int-1, Method, Dm-1, AGAMOUS-like 61, germacrene A synthase activity, Enterococcus coli, Dm Wg, CG5939, Chicken, r3d1, l(2)02657, Wnt-1, DiA, average, BDE, SET, Fresh Frozen Plasmas, Fresh Frozen, Man (Taxonomy), Escherichia/Shigella coli, l(2)k07135, peptides, I, TAF-I, R3D1-S, chemical analysis., R3D1-L, procedures, DIASP, WNT, Wnt, free, DIA, Dia, E. coli, DmelCG4299, PA1, 4-(4-dihexadecylaminostyryl)N-methylpyridium iodide, IGAAD, set, reaction, TNFSF14, R3D1, Methodological Studies, DmelCG10574, scientific observation, C16orf53, 38E.16, Br, RCB0007, Reference Standard, plasma, wnt, GPF, Sp, phapii, gastric antral vascular ectasia (disorder), (+)-germacrene A synthase activity, Standard Preparation, Standard Preparations, Frozen Plasma, Radiation, Standardization, Gastric Antral Vascular Ectasia, UNQ391/PRO726, DmelCG5939, Gallus gallus domesticus, Modern, StF-IT-1, Light, Procedure, SPDSY, Plasmas, HOX4I, Gallus gallus, Multiple Reaction Monitoring, TRBP1, TRBP2, ms(2)04138, Desmoplastic infantile astrocytoma, LIGHT, prm, l(3)S010605, DWint-1, gastric antral vascular ectasia, GAS, 6-trans-farnesyl-diphosphate diphosphate-lyase (germacrene-A-forming) activity, CG4889, Visible Radiations, fg, Visible Radiation, LOQS, HLA-DR-associated protein II, HVEML, DI-2, Standard, 0106/05, I-2Dm, POF2, PM/mPM, F27C12_24, WG, F27C12.24, CG4299, Methodological, Methodological Study, human, I-2PP1, TAF-IBETA, Gallus domesticus, label, DIANA, Wg, TAF-Ibeta, Polypeptide, Growth-potentiating factor, i2pp2a, Fresh, criteria, human being, Fresh Frozen Plasma, Procedures, watermelon stomach, SRML1, dRax, number, 2-trans, high weight, guidelines, presence, PHAPII, Human, Ly113, Loqs, GAVE, Homo sapiens, mPM, DmelCG1768, heavy, chickens, CG6866, Studies, HILDA, Watermelon stomach (disorder), Man, Technique, AXL receptor tyrosine kinase ligand, Blood Plasma, MRM, loq, ipp2a2, HeLa, 2pp2a, dWnt, Visible, Wnt/Wg, CG10574, Phasianus gallus, Wnt1, Study, wnt1, Reference, 2PP2A, taf-ibeta, TRBP, Blood Plasmas, dSET, dSet, peptidos, TR2, Preparation, DIA2, SPS1, measuring, DmelCG6866, Bacterium coli, SPD, l(2)rO727, 6-trans-farnesyl-diphosphate diphosphate-lyase [(+)-germacrene-A-forming] activity, Desmoplastic astrocytoma of infancy, PAPT, igaad, CG1768, DRF2, CD258, (+)-(10R)-germacrene A synthase activity, Peptide, wgl, group, Frozen Plasmas, count in organism, spd, Experiment, I-2PP2A, Bacillus coli, Dm I-2, chemical analysis, MLPLI, I2PP2A, techniques, Dias, Radiations, Plasma, chicken, ensemble, HVEM-L, Photoradiation, cg6866, portion of plasma, LTg, dSET/TAF-Ibeta, Preparations, 2610030F17Rik, Photoradiations, l(3)10631, Bacterium coli commune, Modern Man, Standards, Peptid, bantam, assay, AA407739, DWnt-1, pm, PM, Gla, methodology, CG6866)"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of Stellar MS","description":"A set of plasma peptides were targeted with selected reaction monitoring (SRM) on an Altis Plus and also with parallel reaction monitoring (PRM) on a Stellar. The maximum concurrency of the assay was 624 transitions for the Altis, but only 128 precursors for the Stellar. The quantitative performance of the assays was characterized with a matrix-matched dilution curve of human into chicken plasma, where the Stellar had LOQs and LODs that were on average 12x and 2x better than the Altis.\n\nTargeted assays were created for absolute quantitation of the PQ500 light and heavy peptides for a 60 samples-per-day (SPD) and 100 SPD throughputs. The quantitative performance of these assays was characterized using dilution curves of the heavy standards spiked into 300 ng of human plasma with the Stellar mass spectrometer. Both assays gave good results, with the 60 SPD assay having LOQs about 1.6x better on average than the 100 SPD assay.\n\nTargeted assays were created for label-free quantitation of E. coli spiked into 200 ng of HeLa. Two 60 samples-per-day methods were created, one with 397 peptides, and another with 1302 peptides. These assays were based off of a gas-phase-fractionation (GPF) data independent acquisition (DIA) experiment using the Thermo Fisher Scientific Stellar mass spectrometer. The quantitative performance of these assays was characterized over the range of E. coli amounts from 100 ng to 0.78 ng. Both size assays gave good results, with the n=397 assay having LOQs about 1.2x better on average than the n=1302 assay.","dates":{"publication":"Tue Jun 11 00:00:00 GMT+01:00 2024"},"accession":"PXD052734","cross_references":{"TAXONOMY":["562","9606","9031"]}}