PAXDBapplication/xmlhttp://pax-db.org/downloads/latest/datasets/bioprojects-abundance-files-v4.0.zipprimaryOK2000000ProteomicsChristian von Mering3635https://pax-db.org/dataset/3635/2382479503mering@imls.uzh.chUniversity of ZurichPAXDBFor the rescaling, the datasets are first parsed or processed such that the data reflect proportional abundances of whole protein molecules (i.e. proportionality to counts of complete, individual protein molecules, not to molecular weights, protein volumes, or digested peptides). In the case of spectral counting data protein. The proportional abundances are rescaled linearly to add up to one million; this means the abundance of each protein of interest is finally expressed in (parts per million,) relative to all other proteins in a sample. For a given protein abundance dataset, we then compute the absolute log abundance ratios of all pairs of proteins annotated to be functionally linked. The median of these absolute log abundance ratios represents an indirect quality metric: the closer it is to zero, the better (i.e. the more there is consistency between abundance values and functional annotations such as protein complexes or pathways). We then compute a background expectation for this metric, by permuting the abundance values in a given dataset randomly, and recomputing the median log abundance ratios. The permutation is repeated several times, yielding a distribution of medians. The actually observed median is then expressed as a Z-score distance to the random distribution ofmedians—this distance is termed the interaction consistency score.Reproductive performance in plants is impaired as maximum temperatures consistently approach 40°C. However, the timing of heatwaves critically affects their impact. We studied the molecular responses during pollen maturation in cotton to investigate the vulnerability to high temperature. Tetrads (TEs), uninucleate and binucleate microspores, and mature pollen were subjected to SWATH-MS and RNA-seq analyses after exposure to 38/28°C (day/night) for 5 days. The results indicated that molecular signatures were downregulated progressively in response to heat during pollen development. This was even more evident in leaves, where three-quarters of differentially changed proteins decreased in abundance during heat. Functional analysis showed that translation of genes increased in TEs after exposure to heat; however, the reverse pattern was observed in mature pollen and leaves. For example, proteins involved in transport were highly abundant in TEs whereas in later stages of pollen formation and leaves, heat suppressed synthesis of proteins involved in cell-to-cell communication. Moreover, a large number of heat shock proteins were identified in heat-affected TEs, but these proteins were less abundant in mature pollen and leaves. We speculate that the sensitivity of TE cells to heat is related to high rates of translation targeted to pathways that might not be essential for thermotolerance. Molecular signatures during stages of pollen development after heatwaves could provide markers for future genetic improvement.Patterns of gene expression in pollen of cotton (Gossypium hirsutum) indicate downregulation as a feature of thermotolerance.Masoomi-Aladizgeh Farhad F, McKay Matthew J MJ, Asar Yasmin Y, Haynes Paul A PA, Atwell Brian J BJAdd, DmelCG43443, ADD, ADD-87, Hts-RC, data, AU023367, Data Set, protein complex, supply, Proteins, Ovhts, Gene, HtsRC, CG9325, protein, neutral molecular compounds, protein-containing complex, Dmel_CG9325, Xt, Peptide, 1B1, add, Polypeptides, anon-EST:Posey9, protein polypeptide chains, native protein, peptido, htsRC, GLI3-190, natural protein, polypeptide chain, Add-hts, Protein, CG43443, Gene Products, l(2)k14523, Dmel_CG34197, l(2)00634, median, Ovhts-RC, background, supply and distribution, protein aggregate, all_pairs, molecule, Bph, molecula, Random selection by shearing, oligonucleotide random primer, proportion, HTS-R1, HTS, Hts, molecules, peptides, l(2)k06121, adducin, GLI3FL, distribution, AI854843, proportionality, add-like, HTS-RC, rate, proteins, Molekuel, Pdn, sample population, introduction, Protein Gene Products, Gene Proteins, Adducin, RANDOM, 10^[-6], ppm, sample, supply., quotient, Peptid, peptidos, Polypeptide, Attention Deficit Hyperactivity Disorder, l(2)01103, EST D, CG34197, HtsF, ratiosequence, primary structure of sequence macromolecule.Gossypium purpurascens, Pollen Grain, Plant Male, Male, cotton, Tolerances, Male Gametophytes, Feature, Gene, Gametophyte, Pollen, Male Gamete, Male Gametes, Male Gametophyte, Heat Adaptation., pollen grain (exact), Characteristic, exact), American upland cotton, Gamete, Heat, Gametes, Pollen Grains, Plant Microspores, Grain, Microspores, Gene Expressions, polen (Spanish, Gametophytes, Plant, Features, Expressions, Grains, Plant Male Gametes, male generative cell, Tolerance, Heat Tolerance, Plant Male Gamete, Gossypium lanceolatum, Gossypium hirsutum subsp. mexicanum, 花粉 (Japanese, American cotton, thermotolerance, Expression, upland cotton, Characteristics, Microspore, Plant Microspore, pollen grainGametes, Pollen Grains, Plant Microspores, Grain, Pollen Grain, Microspores, polen (Spanish, Plant Male, Male, Male Gametophytes, Gametophytes, Plant, Gametophyte, Pollen, Male Gamete, Male Gametes, Male Gametophyte, Grains, Plant Male Gametes, pollen grain (exact), male generative cell, exact), Plant Male Gamete, Gamete, sequence, primary structure of sequence macromolecule., 花粉 (Japanese, Microspore, Plant Microspore, pollen grainprotein translation, Gossypium purpurascens, Materials, Plant Male, Male, determination, Mbp1, Pollen, formation of generative and vegetative cells, Circulatory Collapse, Cell Interactions, dysfunctional, dmTAF[[II]]230, solute:solute exchange, exact), Communications, 10.5, Heat, Cell-to-Cell, myd, present in fewer numbers in organism, hereditary., 10.9, multicellular organismal biosynthetic process, Shock, tess-2, increased, single-organism biosynthetic process, Grain, F, Tes1, TFIID TAF250, DmelCG2328, cel, hot, Moods, anabolism, Cell to Cell Interaction, Mbp-1, plants, V, proteins, Futurology, Tes2, NACK2, Communication, genetic, allergic reaction, decreased, 花粉 (Japanese, upland cotton, Predictions and Projections, protein anabolism, dTAF[[II]]230, protein biosynthetic process, gyltl1b-b, Male Gametophytes, land plants, familial, TAF200, RNA-seq, Circulatory Failure, TAFII-250, TAF250/230, results, partial functionality, Male Gametophyte, TAFII250, Interaction, tess, single-organism transport, MDDGA6, mKIAA0609, protein formation, Gamete, Genetic Materials, VI, TESS, KIAA0609, TESS-2, Genetic Material, male gametophyte formation, fg, 20.35, Cell Communications, gyltl1b, ARABIDOPSIS NPK1-ACTIVATING KINESIN 2, mdc1d, Plant, expanded, lacks function of type, CG17603, TAF[[II]], TETRASPORE, low functionality, MDC1D, Grains, higher plants, Plant Male Gametes, enr, Tolerance, protein synthesis, Taf250, enlarged, Specificity and Sensitivity, Material, SR3-5, testin2, microgametophyte development, high temperature, eve2, small molecule transport, Cistron, thermotolerance, inherited genetic, E430016J11Rik, accessory, TAF230, pollen grain, CG2328, 14.10, big, d230, Pollen Grain, Circulatory, number, impaired, Gene, dTAFII250, biosynthesis, Male Gametes, Ximpact, EfW1, supernumerary, froggy, Failure, Gyltl1a, large, reduced, dmTAF1, subnumerary, Taf230, sensitive, Cell-to-Cell Interactions, dTES, Gene Products, Whole Transcriptome Shotgun Sequencing, Mood, Projections and Predictions, even, sensitivity, TAF250, DmelCG6522, Gametes, Pollen Grains, Taf200, Plant Microspores, Microspores, dTAF[[II]]250, polen (Spanish, Genetic, pattern, Cell Interaction, formation, distribution, cell, MDDGB6, dysfunction, Gametophytes, response to heat shock, Cell-to-Cell Interaction, Taf1p, LARGE, decreased number, synthesis, Hypovolemic Shock, dTAF250, BPFD#36, Hypovolemic, male generative cell, pollen grain formation, great, Plant Male Gamete, Gossypium hirsutum subsp. mexicanum, Sensitivity, American cotton, TAF, constitutitional genetic, Plant Microspore, TAF[[II]]250, cotton, Tolerances, synthesize, Affects, Proteins, male gametophyte development, l(3)84Ab, Gametophyte, BG:DS00004.13, Male Gamete, Cistrons, impact-a, Cell, Heat Adaptation, dTAF230, pollen grain (exact), Temperatures, native protein, p230, Protein, chemical analysis, TAF[[II]]250/230, protein biosynthesis, TFIID, Eve, EVE, Taf[[II]]250, TAF[[II]]230, increased number, ATNACK2, Specificity, l(2)46Ce, TAF[II]250, l(2)46Cg, N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate, l(2)46CFj, Protein Gene Products, Collapse, l(2)46CFh, Gene Proteins, present in greater numbers in organism, having decreased function, DmelCG17603, l(2)46CFp, Heat Tolerance, cardinality, Gossypium lanceolatum, NPK1-ACTIVATING KINESIN 2, D6Ertd352e, CG6522, assay, Microspore, E(eve), Future, Interactions, RWDD5, l(2)46CFg, TAF10falseGhirsutum - Pollen, sequence; xls file (Masoomi-aladizgehetal,plantj.2021)abundance based on sequence; xls file, Interaction consistency score: 5.2, Coverage: 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