PAXDBapplication/xmlhttp://pax-db.org/downloads/latest/datasets/bioprojects-abundance-files-v4.0.zipprimaryOK2000000ProteomicsChristian von Mering3635https://pax-db.org/dataset/3635/2549891306mering@imls.uzh.chUniversity of ZurichPAXDBFor the rescaling, the datasets are first parsed or processed such that the data reflect proportional abundances of whole protein molecules (i.e. proportionality to counts of complete, individual protein molecules, not to molecular weights, protein volumes, or digested peptides). In the case of spectral counting data protein. The proportional abundances are rescaled linearly to add up to one million; this means the abundance of each protein of interest is finally expressed in (parts per million,) relative to all other proteins in a sample. For a given protein abundance dataset, we then compute the absolute log abundance ratios of all pairs of proteins annotated to be functionally linked. The median of these absolute log abundance ratios represents an indirect quality metric: the closer it is to zero, the better (i.e. the more there is consistency between abundance values and functional annotations such as protein complexes or pathways). We then compute a background expectation for this metric, by permuting the abundance values in a given dataset randomly, and recomputing the median log abundance ratios. The permutation is repeated several times, yielding a distribution of medians. The actually observed median is then expressed as a Z-score distance to the random distribution ofmedians—this distance is termed the interaction consistency score.<i>Fuzzless-lintless</i> mutant (<i>fl</i>) ovules of upland cotton have been used to investigate cotton fiber development for decades. However, the molecular differences of green tissues between <i>fl</i> and wild-type (WT) cotton were barely reported. Here, we found that gossypol content, the most important secondary metabolite of cotton leaves, was higher in <i>Gossypium hirsutum</i> L. cv Xuzhou-142 (Xu142) WT than in <i>fl</i>. Then, we performed comparative proteomic analysis of the leaves from Xu142 WT and its <i>fl</i>. A total of 4506 proteins were identified, of which 103 and 164 appeared to be WT- and <i>fl</i>-specific, respectively. In the 4239 common-expressed proteins, 80 and 74 were preferentially accumulated in WT and <i>fl</i>, respectively. Pathway enrichment analysis and protein-protein interaction network analysis of both variety-specific and differential abundant proteins showed that secondary metabolism and chloroplast-related pathways were significantly enriched. Quantitative real-time PCR confirmed that the expression levels of 12 out of 16 selected genes from representative pathways were consistent with their protein accumulation patterns. Further analyses showed that the content of chlorophyll a in WT, but not chlorophyll b, was significantly increased compared to <i>fl</i>. This work provides the leaf proteome profiles of Xu142 and its <i>fl</i> mutant, indicating the necessity of further investigation of molecular differences between WT and <i>fl</i> leaves.Comparative Proteomic Analysis of Molecular Differences between Leaves of Wild-Type Upland Cotton and Its <i>Fuzzless</i>-<i>Lintless</i> Mutant.Zhu Liping L, Zheng Bowen B, Song Wangyang W, Tao Chengcheng C, Jin Xiang X, Li Hongbin HAdd, DmelCG43443, ADD, ADD-87, Hts-RC, data, AU023367, Data Set, protein complex, supply, Proteins, Ovhts, Gene, HtsRC, CG9325, protein, neutral molecular compounds, protein-containing complex, Dmel_CG9325, Xt, Peptide, 1B1, add, Polypeptides, anon-EST:Posey9, protein polypeptide chains, native protein, peptido, htsRC, GLI3-190, natural protein, polypeptide chain, Add-hts, Protein, CG43443, Gene Products, l(2)k14523, Dmel_CG34197, l(2)00634, median, Ovhts-RC, background, supply and distribution, protein aggregate, all_pairs, molecule, Bph, molecula, Random selection by shearing, oligonucleotide random primer, proportion, HTS-R1, HTS, Hts, molecules, peptides, l(2)k06121, adducin, GLI3FL, distribution, AI854843, proportionality, add-like, HTS-RC, rate, proteins, Molekuel, Pdn, sample population, introduction, Protein Gene Products, Gene Proteins, Adducin, RANDOM, 10^[-6], ppm, sample, supply., quotient, Peptid, peptidos, Polypeptide, Attention Deficit Hyperactivity Disorder, l(2)01103, EST D, CG34197, HtsF, ratioDAGA4, Gossypium purpurascens, DMDA, DMDA1, cotton, SCARMD2, proteomic analysis, Gossypium lanceolatum., Gossypium hirsutum subsp. mexicanum, A4, American cotton, upland cotton, MAM, SCG3, TYPE, LGMD2Cmolecula., molecules, Molekuel, exact), neutral molecular compounds, molecule, hoja (Spanish, 葉 (Japanesesecondary metabolite metabolic process, Gossypium purpurascens, Materials, (2, determination, Effects, Metabolisms, number, A4, 7'-hexahydroxy-3, Gene, 1', Polymerase Chain Reactions, protein, protein-containing complex, Inverse, composed of, 3'-dimethyl-5, presence, supernumerary, TYPE, Long Term, secondary metabolite metabolism, DAGA4, protein polypeptide chains, Inverse PCR, 2'-Binaphthalene)-8, exact), polypeptide chain, Metabolism, Gene Products, Gossypol Sodium Salt, 1, 6, Polymerase Chain, 7, cotton fiber development, MAM, protein aggregate, SCG3, Effect, increased, network topology analysis, Genetic, Longterm, Gossypol Dipotassium Salt, Tissue, Inverse Polymerase Chain Reaction, study., composition, proteins, Long-Term, (-)-Isomer, Chloroplast, Etioplast, Reaction, Gossypium hirsutum subsp. mexicanum, American cotton, Long-Term Effect, upland cotton, Anchored PCR, Long-Term Effects, accumulated, Anchored Polymerase Chain Reaction, cotton, protein complex, Gossypol, Dipotassium Salt, Proteins, Longterm Effect, total expressed protein, compositionality, Etioplasts, Cistrons, LGMD2C, Secondary Metabolisms, count in organism, native protein, natural protein, American upland cotton, Protein, chemical analysis, Long Term Effects, proteomic analysis, 6', 8'-dicarboxaldehyde, Genetic Materials, secondary metabolism, Genetic Material, PCR, Chlorophyll A2, (+-)-Isomer, Anchored, Reactions, Nested, DMDA1, Nested Polymerase Chain Reaction, increased number, content, common, hoja (Spanish, 葉 (Japanese, Longterm Effects, polymerase chain reaction, Protein Gene Products, 5'-bis(1-methylethyl)-, Gene Proteins, Secondary, present in greater numbers in organism, Sodium Salt, DMDA, (+)-Isomer, Material, SCARMD2, structure, Gossypium lanceolatum, Nested PCR, Cistron, assay, Proteomes, accessory0falseGhirsutum - Leaf, SC (Zhuetal,molecules.2019)abundance based on Spectral counting, Interaction consistency score: 2.5, Coverage: 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