PAXDBapplication/xmlhttp://pax-db.org/downloads/latest/datasets/bioprojects-abundance-files-v4.0.zipprimary200OK0000ProteomicsChristian von Mering8355https://pax-db.org/dataset/8355/299207925mering@imls.uzh.chUniversity of ZurichPAXDBFor the rescaling, the datasets are first parsed or processed such that the data reflect proportional abundances of whole protein molecules (i.e. proportionality to counts of complete, individual protein molecules, not to molecular weights, protein volumes, or digested peptides). In the case of spectral counting data protein. The proportional abundances are rescaled linearly to add up to one million; this means the abundance of each protein of interest is finally expressed in (parts per million,) relative to all other proteins in a sample. For a given protein abundance dataset, we then compute the absolute log abundance ratios of all pairs of proteins annotated to be functionally linked. The median of these absolute log abundance ratios represents an indirect quality metric: the closer it is to zero, the better (i.e. the more there is consistency between abundance values and functional annotations such as protein complexes or pathways). We then compute a background expectation for this metric, by permuting the abundance values in a given dataset randomly, and recomputing the median log abundance ratios. The permutation is repeated several times, yielding a distribution of medians. The actually observed median is then expressed as a Z-score distance to the random distribution ofmedians—this distance is termed the interaction consistency score.While recent developments in genomic sequencing technology have enabled comprehensive transcriptome analyses of single cells, single cell proteomics has thus far been restricted to targeted studies. Here, we perform global absolute protein quantification of fertilized Xenopus laevis eggs using mass spectrometry-based proteomics, quantifying over 5800 proteins in the largest single cell proteome characterized to date. Absolute protein amounts in single eggs are highly consistent, thus indicating a tight regulation of global protein abundance. Protein copy numbers in single eggs range from tens of thousands to ten trillion copies per cell. Comparison between the single-cell proteome and transcriptome reveal poor expression correlation. Finally, we identify 439 proteins that significantly change in abundance during early embryogenesis. Downregulated proteins include ribosomal proteins and upregulated proteins include basal transcription factors, among others. Many of these proteins do not show regulation at the transcript level. Altogether, our data reveal that the transcriptome is a poor indicator of the proteome and that protein levels are tightly controlled in X. laevis eggs.Global absolute quantification reveals tight regulation of protein expression in single Xenopus eggs.Smits Arne H AH, Lindeboom Rik G H RG, Perino Matteo M, van Heeringen Simon J SJ, Veenstra Gert Jan C GJ, Vermeulen Michiel MAdd, DmelCG43443, ADD, ADD-87, Hts-RC, data, AU023367, Data Set, protein complex, supply, Proteins, Ovhts, Gene, HtsRC, CG9325, protein, neutral molecular compounds, protein-containing complex, Dmel_CG9325, Xt, Peptide, 1B1, add, Polypeptides, anon-EST:Posey9, protein polypeptide chains, native protein, peptido, htsRC, GLI3-190, natural protein, polypeptide chain, Add-hts, Protein, CG43443, Gene Products, l(2)k14523, Dmel_CG34197, l(2)00634, median, Ovhts-RC, background, supply and distribution, protein aggregate, all_pairs, molecule, Bph, molecula, Random selection by shearing, oligonucleotide random primer, proportion, HTS-R1, HTS, Hts, molecules, peptides, l(2)k06121, adducin, GLI3FL, distribution, AI854843, proportionality, add-like, HTS-RC, rate, proteins, Molekuel, Pdn, sample population, introduction, Protein Gene Products, Gene Proteins, Adducin, RANDOM, 10^[-6], ppm, sample, supply., quotient, Peptid, peptidos, Polypeptide, Attention Deficit Hyperactivity Disorder, l(2)01103, EST D, CG34197, HtsF, ratio12.1.Xenopus, Xenopus <subgenus>., Xenopus <genus>, gene regulation, regulation of protein expression, regulation of gene product expressionEmbryonic, Presumptive, Embryo, Prenatal Structures, developmental tissue, embryonic organism, embryo, plant germ, Presumptive Structure, Embryos, Prenatal, Embryonic Structure, GRO:0005343, Presumptive Structures., developing organism, Structure, Prenatal StructurePercutaneous Neuromodulation Therapies, Postnidation Embryo, Pre-implantation Embryo Development, Gene Expression Profile, Post-implantation, Ribosomal Protein, protein, Profiles, Pre implantation Embryo Development, Social Controls, DmelCG6383, Embryonic Programming, Transcript Expression Analysis, dmTAF[[II]]230, protein polypeptide chains, Embryo Development, Electrostimulation, Percutaneous Electric Nerve Stimulation, 1802), Gene Expression Monitorings, Percutaneous, Analysis, Profilings, protein aggregate, Fs(3)Hor, Formal Social Controls, Mass Spectrum Analysis, Ribosomal, DmelCG2684, TFIID TAF250, reference sample, Analyses, X. laevis., cel, crumb, proteins, NTef2, Signatures, Preimplantation Embryo Development, Social, Gene Expression Analysis, Xenopus laevis (Daudin, Expression Signature, Transdermal, Transcriptomes, Reagents, Transcutaneous Electrical Stimulation, Therapy, Electric Stimulation, dTAF[[II]]230, Transcutaneous Electrical Nerve Stimulation, embryonal development, Transcutaneous, CG6383, Neuromodulations, Neuromodulation, African clawed frog, Arts, Expression Profiles, Monitorings, TAF200, common platanna, clawed frog <Xenopus laevis>, TAFII-250, TAF250/230, Spectrum Analysis, INSDC_feature:misc_RNA, Electrical Neuromodulation, Fs(3)Sz11, Spectroscopy, Percutaneous Electrical Neuromodulation, Gene Expression, Analgesic Cutaneous Electrostimulation, TAFII250, Expression Signatures, embryo Ce, Transcutaneous Nerve, Expression Profile, Transcriptome Profiles, Percutaneous Electrical, Industrial, Factors, Industrial Arts, Transdermal Electrostimulation, Stimulation, Transcriptome Profilings, Transcutaneous Nerve Stimulation, Spectrometry, Control, Controls, CG17603, TAF[[II]], early, Electrical Neuromodulations, Gene Expression Analyses, Cutaneous Electrostimulation, Electroanalgesia, Taf250, Percutaneous Electrical Neuromodulations, SR3-5, Horka, Indicator, CG2684, Fs(3)Horka, Expression Analysis, Regulation, l(3)S050920, Proteomes, Postimplantation, Embryonic Programmings, TAF230, Platannas, Crbs, Regulations, protein levels, d230, Peptidomics, Transcriptome Profile, Reagent, embryogenesis, Gene, dTAFII250, far, Spectrum Analyses, Development, protein-containing complex, EfW1, Transcription Factor, Gene Expression Profilings, Prenidation Embryo Development, mRNA Differential Displays, Gene Expression Pattern Analysis, Crumbs, polypeptide chain, dmTAF1, Taf230, Embryonic Developments, Gene Products, Mass, clawed frog, DmF2, Animal, Reagents and Indicators, Mass Spectroscopy, TAF250, lod, Postnidation, Taf200, CT19912, Transcription, 0509/20, dTAF[[II]]250, cell, Profile, 1384/04, Neuromodulation Therapy, Postnidation Embryo Development, Transcriptomics, Taf1p, Pre-implantation, Transcript Expression, Transcriptome Analysis, Indicators, Monitoring, dTAF250, embryogenesis and morphogenesis, Electroanalgesias, Electrical Stimulation, Analgesic, Percutaneous Neuromodulation, Expression Analyses, TAF, l(3)j1B5, Controlled, Profiling, data, Controlling, TAF[[II]]250, Transcriptome, Formal Social Control, protein complex, X. laevis, Percutaneous Electrical Nerve Stimulation, Proteins, Post implantation Embryo Development, total expressed protein, Post-implantation Embryo Development, Preimplantation, l(3)84Ab, Nerve Stimulation, BG:DS00004.13, Factor, Cell, Transcutaneous Electric, dTAF230, l(3)07207, whole transcriptome, Differential Display, Transcript Expression Analyses, MS, native protein, natural protein, Social Control, p230, Protein, Gene Expression Signatures, TAF[[II]]250/230, TFIID, CRB, Crb, Bufo laevis, Xenopus leavis, Gene Expression Signature, l(3)S058104, mRNA Differential Display, Mass Spectrum Analyses, Postimplantation Embryo Development, Percutaneous Neuromodulation Therapy, embryonic stage, Embryonic, Taf[[II]]250, Mass Spectrum, Embryo, Gene Expression Monitoring, TAF[[II]]230, Transcriptome Profiling, mRNA, Analgesic Cutaneous, TENS, Embryogenesis, TAF[II]250, Differential Displays, Lds, Transcriptome Analyses, Protein Gene Products, Gene Proteins, DmelCG17603, Gene Expression Profiles, Transcutaneous Electric Stimulation, platanna, regulation, Signature, X. laevi, Platanna, TAF10falseXlaevis - Embryo, SC (Smitsetal,nar2014)abundance based on Spectral counting, Interaction consistency score: 12.1, Coverage: 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