PeptideAtlasapplication/xmlftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Halobacterium/PAe000224/PAe000224_RAW.tarftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Halobacterium/PAe000224/PAe000224_READMEftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Halobacterium/PAe000224/PAe000224_mzXML_201104181135.tar.gzftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Halobacterium/PAe000224/PAe000224_mzXML_201104181135.propertiesftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Halobacterium/PAe000224/PAe000224_222_sequest.paramsftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Halobacterium/PAe000224/PAe000224_prot_222_201104181135.propertiesftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Halobacterium/PAe000224/PAe000224_Search_Results_222_201104181135.tar.gzftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Halobacterium/PAe000224/PAe000224_Search_Results_222_201104181135.propertiesftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Halobacterium/PAe000224/PAe000224_prot_222_201104181135.tar.gzftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Halobacterium/PAe000224/PAe000224_222_search.params.tar.gzprimary200OKY. A. Goo, E. C. Yi, N. S. Baliga, W. A. Tao, M. Pan, R. Aebersold, D. R. Goodlett, L. Hood and W. V. NgTPP'https://db.systemsbiology.net/sbeams/cgi/PeptideAtlas/ManageTable.cgi?TABLE_NAME=AT_sample&sample_id=251'nbaliga@systemsbiology.orgPeptideAtlasProteomicsUnknownHalobacterium Nrc-1 (halobacterium)This paper reports on the findings of the Biomedical Research Institute, as one of the participants in the pilot study of the HUPO Brain Proteome Project. A biopsy and autopsy study sample derived from human brain was distributed among the participants for proteomic analysis. In our laboratory, attention was focused on protein identification using the bottom-up shotgun approach. Protein extracts derived from both samples were trypsinized and analyzed separately by 2-D LC and MS. In a complementary approach, the tryptic digests were analyzed directly by LC-ESI-MS/MS and gas-phase fractionation in the mass spectrometer. Taken together, both proteomic approaches in combination with a stringent evaluation process, resulted in the confident identification of 209 proteins in the human brain samples under investigation.Gel-free analysis of the human brain proteome: application of liquid chromatography and mass spectrometry on biopsy and autopsy samples.Dumont Debora D, Noben Jean-Paul JP, Verhaert Peter P, Stinissen Piet P, Robben Johan JfalseYAGsn1enrichment for soluble proteins; Goo et al., 2003, Mol Cell Proteomics 2(8):5052006-12-312016-03-29PAe0002241691297064091