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However, important research directions, particularly those involving mathematical modelling and simulation of biological processes, also critically depend on absolutely quantitative data--that is, knowledge of the concentration of the expressed proteins as a function of cellular state. Until now, absolute protein concentration measurements of a considerable fraction of the proteome (73%) have only been derived from genetically altered Saccharomyces cerevisiae cells, a technique that is not directly portable from yeast to other species. Here we present a mass-spectrometry-based strategy to determine the absolute quantity, that is, the average number of protein copies per cell in a cell population, for a large fraction of the proteome in genetically unperturbed cells. Applying the technology to the human pathogen Leptospira interrogans, a spirochete responsible for leptospirosis, we generated an absolute protein abundance scale for 83% of the mass-spectrometry-detectable proteome, from cells at different states. Taking advantage of the unique cellular dimensions of L. interrogans, we used cryo-electron tomography morphological measurements to verify, at the single-cell level, the average absolute abundance values of selected proteins determined by mass spectrometry on a population of cells. Because the strategy is relatively fast and applicable to any cell type, we expect that it will become a cornerstone of quantitative biology and systems biology."],"pubmed_title":["Proteome-wide cellular protein concentrations of the human pathogen Leptospira interrogans."],"pubmed_authors":["Malmström Johan J, Beck Martin M, Schmidt Alexander A, Lange Vinzenz V, Deutsch Eric W EW, Aebersold Ruedi R"],"additional_accession":[]},"is_claimable":false,"name":"Li_OGE3-10_LTQ","description":"Li_OGE3-10_LTQ","dates":{"publication":"2009-12-31","export":"2016-03-29"},"accession":"PAe000351","cross_references":{"pubmed":["19606093"],"IPI":["IPI00009867","IPI00019359","IPI00021304","IPI00217963","IPI00220327","IPI00240020","IPI00295684"],"NCBI":["45655586","45655587","45655588","45655589","45655592","45655593","45655594","45655596","45655598","45655603","45655604","45655605","45655609","45655610","45655611","45655613","45655614","45655615","45655616","45655618","45655620","45655626","45655627","45655628","45655630","45655631","45655632","45655633","45655637","45655640","45655641","45655643","45655644","45655645","45655647","45655648","45655649","45655650","45655651","45655659","45655660","45655661","45655662","45655663","45655666","45655668","45655669","45655670","45655671","45655672","45655673","45655675","45655676","45655679","45655680","45655681","45655683","45655684","45655691","45655692","45655695","45655697","45655698","45655706","45655709","45655713","45655715","45655719","45655720","45655723","45655724","45655725","45655726","45655730","45655733","45655735","45655738","45655740","45655741","45655743","45655749","45655750","45655754","45655757","45655767","45655769","45655772","45655773","45655774","45655775","45655776","45655777","45655778","45655779","45655780","45655784","45655785","45655788","45655789","45655790","45655794","45655802","45655805","45655806","45655807","45655810","45655814","45655815","45655816","45655817","45655819","45655820","45655822","45655824","45655825","45655828","45655829","45655831","45655834","45655839","45655840","45655841","45655845","45655847","45655848","45655849","45655850","45655916","45655919","45655920","45655923","45655925","45655926","45655928","45655931","45655933","45655934","45655936","45655937","45655940","45655943","45655944","45655946","45655951","45655952","45655953","45655954","45655957","45655959","45655960","45655962","45655963","45655964","45655965","45655967","45655969","45655978","45655979","45655980","45655982","45655983","45655986","45655987","45655988","45655989","45655991","45655993","45656005","45656008","45656012","45656013","45656015","45656016","45656017","45656022","45656023","45656024","45656025","45656027","45656028","45656031","45656032","45656033","45656034","45656035","45656037","45656040","45656041","45656042","45656044","45656045","45656047","45656048","45656050","45656051","45656053","45656054","45656055","45656058","45656063","45656064","45656066","45656067","45656068","45656069","45656072","45656073","45656079","45656080","45656081","45656082","45656083","45656084","45656086","45656087","45656089","45656091","45656092","45656094","45656096","45656097","45656098","45656099","45656100","45656103","45656105","45656108","45656111","45656113","45656114","45656119","45656121","45656125","45656126","45656127","45656129","45656130","45656131","45656133","45656138","45656140","45656141","45656142","45656146","45656149","45656156","45656157","45656158","45656159","45656163","45656164","45656171","45656172","45656173","45656174","45656176","45656184","45656185","45656186","45656187","45656191","45656192","45656197","45656198","45656199","45656204","45656205","45656206","45656213","45656214","45656215","45656218","45656219","45656221","45656222","45656227","45656229","45656230","45656231","45656240","45656241","45656243","45656248","45656252","45656253","45656254","45656260","45656261","45656262","45656263","45656264","45656266","45656267","45656269","45656280","45656282","45656283","45656286","45656287","45656289","45656290","45656296","45656298","45656302","45656303","45656306","45656309","45656311","45656315","45656319","45656325","45656326","45656327","45656328","45656329","45656330","45656331","45656332","45656333","45656339","45656342","45656343","45656344","45656345","45656349","45656350","45656356","45656357","45656358","45656362","45656363","45656368","45656370","45656371","45656373","45656380","45656381","45656382","45656384","45656403","45656410","45656415","45656416","45656419","45656420","45656421","45656422","45656423","45656424","45656427","45656436","45656441","45656443","45656444","45656445","45656446","45656447","45656448","45656449","45656452","45656455","45656456","45656463","45656466","45656468","45656469","45656472","45656476","45656479","45656480","45656481","45656484","45656485","45656489","45656490","45656494","45656497","45656498","45656499","45656500","45656503","45656504","45656505","45656506","45656508","45656510","45656513","45656516","45656518","45656519","45656520","45656522","45656523","45656524","45656526","45656527","45656529","45656530","45656531","45656532","45656534","45656538","45656539","45656541","45656553","45656555","45656559","45656560","45656561","45656562","45656563","45656564","45656565","45656566","45656569","45656570","45656571","45656576","45656577","45656579","45656581","45656588","45656593","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