PeptideAtlasapplication/xmlftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Candida_albicans/PAe001987/PAe001987_5558_tandem.fastaftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Candida_albicans/PAe001987/PAe001987_prot_5558_201307080502.tar.gzftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Candida_albicans/PAe001987/PAe001987_raw_201307080451.tar.gzftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Candida_albicans/PAe001987/PAe001987_5558_tandem.paramsftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Candida_albicans/PAe001987/PAe001987_prot_5558_201307080502.propertiesftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Candida_albicans/PAe001987/PAe001987_Search_Results_5558_201307080502.propertiesftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Candida_albicans/PAe001987/PAe001987_READMEftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Candida_albicans/PAe001987/PAe001987_mzML_201307080417.tar.gzftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Candida_albicans/PAe001987/PAe001987_mzML_201307080417.propertiesftp://ftp.peptideatlas.org/pub/PeptideAtlas/Repository/Candida_albicans/PAe001987/PAe001987_Search_Results_5558_201307080502.tar.gzprimaryOK200Vital VialasTPP'https://db.systemsbiology.net/sbeams/cgi/PeptideAtlas/ManageTable.cgi?TABLE_NAME=AT_sample&sample_id=3247'PeptideAtlasProteomicsLTQ Orbitrap VelosCandida AlbicansCandida albicans public proteomic datasets, though growing steadily in the last few years, still have a very limited presence in online repositories. We report here the creation of a C. albicans PeptideAtlas comprising near 22,000 distinct peptides at a 0.24% False Discovery Rate (FDR) that account for over 2500 canonical proteins at a 1.2% FDR. Based on data from 16 experiments, we attained coverage of 41% of the C. albicans open reading frame sequences (ORFs) in the database used for the searches. This PeptideAtlas provides several useful features, including comprehensive protein and peptide-centered search capabilities and visualization tools that establish a solid basis for the study of basic biological mechanisms key to virulence and pathogenesis such as dimorphism, adherence, and apoptosis. Further, it is a valuable resource for the selection of candidate proteotypic peptides for targeted proteomic experiments via Selected Reaction Monitoring (SRM) or SWATH-MS.<h4>Biological significance</h4>This C. albicans PeptideAtlas resolves the previous absence of fungal pathogens in the PeptideAtlas project. It represents the most extensive characterization of the proteome of this fungus that exists up to the current date, including evidence for uncharacterized ORFs. Through its web interface, PeptideAtlas supports the study of interesting proteins related to basic biological mechanisms key to virulence such as apoptosis, dimorphism and adherence. It also provides a valuable resource to select candidate proteotypic peptides for future (SRM) targeted proteomic experiments. This article is part of a Special Issue entitled: Trends in Microbial Proteomics.A Candida albicans PeptideAtlas.Vialas Vital V, Sun Zhi Z, Loureiro y Penha Carla Verónica CV, Carrascal Montserrat M, Abián Joaquín J, Monteoliva Lucía L, Deutsch Eric W EW, Aebersold Ruedi R, Moritz Robert L RL, Gil Concha CProcandida stellatoidea, SRML1, Feature, selection process, regulation by symbiont of host system process, caspase-dependent programmed cell death, number, positive regulation by symbiont of host non-apoptotic programmed cell death, Gene, Caspase Dependent Apoptosis, Monilia stellatoidea, Progress Reports, protein, protein-containing complex, type I programmed cell death, presence, infectivity, dIKK-gamma, activation of apoptosis, peptide, Polypeptides, Investigative, peptido, Extrinsic Pathway Apoptoses, Dematium albicans, induction by organism of non-apoptotic programmed cell death in other organism during symbiotic interaction, Summary Report, DmIKK-gamma, Gene Products, pathogenesis, Projections and Predictions, dmIKKgamma, Type I, protein aggregate, IKK[[gamma]], Summary Reports, IKKg, KEY, Key, Monilia albicans, study, Pilz, viral infection, Filamentous Fungi, peptides, stimulation by symbiont of host programmed cell death, MRM, Progress Report, Candida stellatoidea type I, absent from organism, Futurology, causes, Filamentous Fungus, apoptosis activator activity, Progress, Intrinsic Pathway Apoptosis, forecasting, Field Reports, IKK, Classic, Classic Apoptosis, Mycotorula albicans, Classical Apoptosis, causality, peptidos, Characteristics, fungi, Investigative Reports, Parasaccharomyces albicans, SPS1, Apoptosis, Predictions and Projections, Peptidomics., visualization, activation by organism of non-apoptotic programmed cell death in other organism, Molds, Caspase-Dependent Apoptosis, hemolysin activity, protein complex, Proteins, PAPT, Fungi, Fungus, total expressed protein, Candida albicans var. stellatoidea, Syringospora albicans, SPDSY, Peptide, Mold, Multiple Reaction Monitoring, futurology, polypeptide, IKKgamma, count in organism, DmIKKgamma, Characteristic, signaling (initiator) caspase activity, native protein, induction of apoptosis, Investigative Report, dIKK, Intrinsic Pathway, Procandida albicans, Programmed Cell Death, Extrinsic Pathway Apoptosis, Research Reports, Protein, Kenny, Pathogenicity, modulation by symbiont of host system process, Caspase-Dependent, Data Base, apoptosis signaling, absence, Saccharomyces albicans, distinct, apoptosis, Oidium albicans, Dmikkgamma, Field, IKK-gamma, fungus, Features, CG16910, Protein Gene Products, Classic Apoptoses, Intrinsic Pathway Apoptoses, Gene Proteins, Endomyces albicans, Report, DmelCG16910, induction of apoptosis by p53, Reports, Filamentous, apoptotic program, activation by symbiont of host programmed cell death, Extrinsic Pathway, commitment to apoptosis, Peptid, Candida stellatoidea, virulence, Polypeptide, Summary, Future, Classical, Apoptoses, Proteomes, Field Report, Myceloblastanon albicansl(4)17, sodium salt, Ce, l(4)13, Gli, DmelCG8566, Dunc104, Ci, Cid[Mel], TSC-1, Ci[D], imidazole-4-acetic acid, cenH3, DmKlp53D, KIF1B, CENP-A, collisionally activated dissociation, Cid, CENP-C, CID, ciD, CenpA, l(4)102ABc, 1H-imidazoleacetic acid, CAD, ci[D], Ci155, Unc104, ci-D, CG8566, Siah, cenpA, CenH3[Cid], CG13329, CenH3[CID], imidazole-4-acetic acid hydrochloride, BcDNA:RE21270, DmelCG2125, CenH3, ci155, CenH3/CID, Ci/Gli, Ci/GLI, CENP-A/Cid, CENP-A/CID, CENPA, Unc-104., imidazolyl-4-acetic acid, DmelCG13329, MIP-4alpha, Klp53D, X-linked combined immunodeficiency, IMAC, DmCG8566, CI, SCYA26, MIP-4a, Cenp-A, CG2125, imac, CenpA/CID, Ci-155, CID/CENP-A, CiDEndomyces albicans, Saccharomyces albicans, Procandida stellatoidea, Dematium albicans, Oidium albicans, Candida stellatoidea type I, Procandida albicans, Monilia albicans., Mycotorula albicans, Candida stellatoidea, Candida albicans var. stellatoidea, Monilia stellatoidea, Syringospora albicans, Parasaccharomyces albicans, Myceloblastanon albicansfalseSILAC_phos_OrbitrapVelos_1Orbitrap Velos-CID. SILAC IMAC&TiO2. Replicate 12013-12-312016-03-29PAe00198723811049sp|ADH1_YEAST|sp|CAS1_BOVIN|sp|CASB_BOVIN|sp|K1C10_HUMAN|sp|K1C9_HUMAN|sp|K22E_HUMAN|sp|K2C1_HUMAN|sp|RS27A_HUMAN|sp|SYH_HUMAN|sp|TRY1_BOVIN|sp|TRYP_PIG|