PeptideAtlasTPP'https://db.systemsbiology.net/sbeams/cgi/PeptideAtlas/ManageTable.cgi?TABLE_NAME=AT_sample&sample_id=5234'PeptideAtlasProteomicsLTQMus MusculusE14.Tg2a mouse embryonic stem (mES) cells are a widely used host in gene trap and gene targeting techniques. Molecular characterization of host cells will provide background information for a better understanding of functions of the knockout genes. Using a highly selective glycopeptide-capture approach but ordinary liquid chromatography coupled mass spectrometry (LC-MS), we characterized the N-glycoproteins of E14.Tg2a cells and analyzed the close relationship between the obtained N-glycoproteome and cell-surface proteomes. Our results provide a global view of cell surface protein molecular properties, in which receptors seem to be much more diverse but lower in abundance than transporters on average. In addition, our results provide a systematic view of the E14.Tg2a N-glycosylation, from which we discovered some striking patterns, including an evolutionarily preserved and maybe functionally selected complementarity between N-glycosylation and the transmembrane structure in protein sequences. We also observed an environmentally influenced N-glycosylation pattern among glycoenzymes and extracellular matrix proteins. We hope that the acquired information enhances our molecular understanding of mES E14.Tg2a as well as the biological roles played by N-glycosylation in cell biology in general.N-glycoproteome of E14.Tg2a mouse embryonic stem cells.Sun Bingyun B, Ma Li L, Yan Xiaowei X, Lee Denis D, Alexander Vinita V, Hohmann Laura J LJ, Lorang Cynthia C, Chandrasena Lalangi L, Tian Qiang Q, Hood Leroy LfalseMm_mESC_glycoMm_mESC_glyco2013-12-312016-03-29PAe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