PeptideAtlasSigdel TK, Kaushal A, Gritsenko M, Norbeck AD, Qian WJ, Xiao W, Camp DG, Smith RD, Sarwal MMTPP'https://db.systemsbiology.net/sbeams/cgi/PeptideAtlas/ManageTable.cgi?TABLE_NAME=AT_sample&sample_id=5360'UrinePeptideAtlasProteomicsLTQHomo Sapiens (human)<h4>Purpose</h4>Acute rejection (AR) remains the primary risk factor for renal transplant outcome; development of non-invasive diagnostic biomarkers for AR is an unmet need.<h4>Experimental design</h4>We used shotgun proteomics applying LC-MS/MS and ELISA to analyze a set of 92 urine samples, from patients with AR, stable grafts (STA), proteinuria (NS), and healthy controls.<h4>Results</h4>A total of 1446 urinary proteins (UP) were identified along with a number of nonspecific proteinuria-specific, renal transplantation specific and AR-specific proteins. Relative abundance of identified UP was measured by protein-level spectral counts adopting a weighted fold-change statistic, assigning increased weight for more frequently observed proteins. We have identified alterations in a number of specific UP in AR, primarily relating to MHC antigens, the complement cascade and extra-cellular matrix proteins. A subset of proteins (uromodulin, SERPINF1 and CD44), have been further cross-validated by ELISA in an independent set of urine samples, for significant differences in the abundance of these UP in AR.<h4>Conclusions and clinical relevance</h4>This label-free, semi-quantitative approach for sampling the urinary proteome in normal and disease states provides a robust and sensitive method for detection of UP for serial, non-invasive clinical monitoring for graft rejection after kidney transplantation.Shotgun proteomics identifies proteins specific for acute renal transplant rejection.Sigdel Tara K TK, Kaushal Amit A, Gritsenko Marina M, Norbeck Angela D AD, Qian Wei-Jun WJ, Xiao Wenzhong W, Camp David G DG, Smith Richard D RD, Sarwal Minnie M MMfalseH_UrinaryProt_CtrlHuman urinary proteins, healthy 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