{"database":"PeptideAtlas","file_versions":[],"scores":null,"additional":{"submitter":[""],"disease":[""],"software":["TPP"],"full_dataset_link":["'https://db.systemsbiology.net/sbeams/cgi/PeptideAtlas/ManageTable.cgi?TABLE_NAME=AT_sample&sample_id=6883'"],"synonyms":[""],"submitter_email":[""],"repository":["PeptideAtlas"],"sample_protocol":[""],"data_protocol":[""],"omics_type":["Proteomics"],"pubmed":[""],"instrument_platform":["LTQ Orbitrap"],"project_tag":[""],"species":["Caenorhabditis Elegans (c. Elegans)"],"publication":[""],"pubmed_abstract":["MicroRNAs are regulators of gene expression whose functions are critical for normal development and physiology. We have previously characterized mutations in a Caenorhabditis elegans microRNA-specific Argonaute ALG-1 (Argonaute-like gene) that are antimorphic [alg-1(anti)]. alg-1(anti) mutants have dramatically stronger microRNA-related phenotypes than animals with a complete loss of ALG-1. ALG-1(anti) miRISC (microRNA induced silencing complex) fails to undergo a functional transition from microRNA processing to target repression. To better understand this transition, we characterized the small RNA and protein populations associated with ALG-1(anti) complexes in vivo. We extensively characterized proteins associated with wild-type and mutant ALG-1 and found that the mutant ALG-1(anti) protein fails to interact with numerous miRISC cofactors, including proteins known to be necessary for target repression. In addition, alg-1(anti) mutants dramatically overaccumulated microRNA* (passenger) strands, and immunoprecipitated ALG-1(anti) complexes contained nonstoichiometric yields of mature microRNA and microRNA* strands, with some microRNA* strands present in the ALG-1(anti) Argonaute far in excess of the corresponding mature microRNAs. We show complex and microRNA-specific defects in microRNA strand selection and microRNA* strand disposal. For certain microRNAs (for example mir-58), microRNA guide strand selection by ALG-1(anti) appeared normal, but microRNA* strand release was inefficient. For other microRNAs (such as mir-2), both the microRNA and microRNA* strands were selected as guide by ALG-1(anti), indicating a defect in normal specificity of the strand choice. Our results suggest that wild-type ALG-1 complexes recognize structural features of particular microRNAs in the context of conducting the strand selection and microRNA* ejection steps of miRISC maturation."],"pubmed_title":["Caenorhabditis elegans ALG-1 antimorphic mutations uncover functions for Argonaute in microRNA guide strand selection and passenger strand disposal."],"pubmed_authors":["Zinovyeva Anna Y AY, Veksler-Lublinsky Isana I, Vashisht Ajay A AA, Wohlschlegel James A JA, Ambros Victor R VR"],"pubmed_title_synonyms":["pri miRNA, RNA, miRNAs, Small Temporal, Micro, elegan, Temporal RNA, stRNA, Small Temporal RNA, Primary MicroRNA, Primary, Caenorhabditis, miRNA, Primary miRNA, Caenorhabditis elegan, INSDC_feature:ncRNA, pri-miRNA, Mutations, nematode, Small, Micro RNA, MicroRNA, selection process., pre-miRNA, Rhabditis elegans, pre miRNA"],"pubmed_abstract_synonyms":["Crbs, HH, MGC130048, Ribonucleic, Materials, SGCG_HUMAN, single-organism developmental process, elegan, selection process, Feature, stRNA, postnatal development, metazoans, bar, A4, Gene, growth and development, far, protein, protein-containing complex, IDOL, animals, cofactors, TYPE, DmelCG6383, Hh, Mutations, DAGA4, Small, anon-WO0134654.19, DmelCG4637, reduced, Crumbs, 35DAG, Gene Products, Animal, tiny, Mir, MIR, protein aggregate, MAM, gamma-SG, SCG3, Non Polyadenylated, RNA Gene Products, Animalia, Miranda, average, gamma sarcoglycan, CT19912, 0509/20, Small Temporal, Gene Expressions, Genetic, MONDOA, Mrt, 1384/04, hypoplasia, crumb, mir, miRNA, Primary miRNA, Expressions, Caenorhabditis elegan, pri-miRNA, INSDC_feature:ncRNA, Non-Polyadenylated RNA, nematode, reaction, Myosin regulatory light chain-interacting protein, bar-3, gamma-sarcoglycan, Expression, Characteristics, bHLHe36, Idol, pre miRNA, miRNA biogenesis, l(3)j1B5, small, 9430057C20Rik, RNA, l(3)hh, 35 kDa dystrophin-associated glycoprotein, CG6383, ribose nucleic acid, Myosin regulatory light chain interacting protein, protein complex, 6.3.2.-, Proteins, ribonucleic acids, SG-gamma, Small Temporal RNA, RNS, Primary, function, Cistrons, results, SGCG, LGMD2C, CG4637, l(3)07207, CG12249, development, AW228700, Micro RNA, Characteristic, native protein, yeast nucleic acid, Ribonukleinsaeure, Metazoa, Protein, bar3, Genetic Materials, pentosenucleic acids, CRB, Crb, sarcoglycan, Primary., Ribonucleic acids, DmelCG12249, pre-miRNA, l(3)S058104, Rhabditis elegans, Genetic Material, pri miRNA, ribonucleic acid, Acid, miRNAs, cofactor, l(3)neo56, l(3)neo57, DMDA1, Micro, underdeveloped, Temporal RNA, Non Polyadenylated RNA, Mira, postnatal growth, Non-Polyadenylated, Primary MicroRNA, INSDC_feature:gene, Inducible degrader of the LDL-receptor, Ribonucleic Acid, Caenorhabditis, gamma (35kDa dystrophin-associated glycoprotein), Features, loss of, Protein Gene Products, multicellular animals, Phenotypes, Gene Proteins, DMDA, anon-WO0182946.19, Material, 35kD dystrophin-associated glycoprotein, SCARMD2, MIRA, MicroRNA, Cistron, growth, l(3)S050920, hg"],"additional_accession":[]},"is_claimable":false,"name":"alg-1-null","description":"alg-1(null)","dates":{"publication":"2015-12-31","export":"2016-03-29"},"accession":"PAe005610","cross_references":{"pubmed":["26351692"],"UniProt":["A3QMC5","A5Z2S4","A5Z2W3","A7DT45","A9UJN7","B1Q273","B2FD95","B3CJ34","B3GWA1","B6EU49","B7FAR9","C1P640","C6KRJ3","C6KRP5","C9IY21","D0IMZ5","D0VWL6","D1MN85","D3YT36","D5MCN2","E9RKC7","F3Y5Q2","F3Y5Q7","G1K0V8","G4S034","G4SF79","G4SLH0","G4SPY0","G5EBF4","G5EBH3","G5EBH7","G5EBI0","G5EBP5","G5EBR1","G5EBR5","G5EBV6","G5EBY3","G5EBY8","G5EBZ4","G5EC16","G5EC78","G5ECC9","G5ECL3","G5ECM6","G5ECU1","G5ECU5","G5ECZ0","G5ED07","G5ED27","G5ED29","G5ED36","G5ED41","G5ED99","G5EDA1","G5EDA3","G5EDD3","G5EDE4","G5EDP2","G5EDQ4","G5EDS2","G5EDV3","G5EDZ9","G5EE04","G5EE23","G5EE32","G5EE66","G5EEA8","G5EEG8","G5EEI4","G5EEK2","G5EEK9","G5EEL0","G5EEL2","G5EEQ8","G5EER7","G5EES3","G5EES9","G5EEV2","G5EEY5","G5EF32","G5EF37","G5EF53","G5EF84","G5EF87","G5EFC4","G5EFC8","G5EFE3","G5EFI4","G5EFJ3","G5EFK7","G5EFS2","G5EFZ1","G5EGK1","G5EGK8","G5EGS5","G8JY24","H1UBJ9","H2FLK7","H2KY58","H2KYB8","H2KYK4","H2KYL8","H2KYP1","H2KYR1","H2KYU6","H2KYV3","H2KZ06","H2KZ18","H2KZD5","H2KZL7","H2KZR3","H2L023","H2L080","H2L0A4","H2L0A8","H2L0E0","H2L0I3","H2L0I7","H2L0M0","H2L2A2","H9G2T4","I2HAG1","I7FW32","K8ERV5","K8ESK7","K8ESL3","K8FDX2","K8FE02","L8E6Y3","N1NTD8","O01479-2","O01504","O01530","O01583","O01590","O01692","O01761","O01802","O01806","O01812","O01868","O01869","O01974","O02056","O02108","O02141","O02215","O02220","O02286","O02328","O02365","O02639","O02640","O16259","O16266","O16303","O16305","O16368","O16517","O16689","O16720","O17005","O17071-2","O17214","O17218","O17566","O17570","O17586","O17607","O17612","O17626","O17687","O17694","O17695","O17732","O17759","O17836","O17869","O17919","O17953","O18000","O18054","O18180","O18221","O18229-2","O18240","O18307","O18650","O44144","O44145","O44156","O44400","O44441","O44451","O44480","O44503","O44572","O44741","O44906","O44952","O44954","O44956","O45011","O45012","O45087","O45148","O45227","O45246","O45279","O45319","O45406","O45430","O45499","O45502","O45544","O45552","O45599","O45679","O45734","O45812","O45815","O45865","O45924","O45935","O45941","O45946","O61199","O61208","O61235","O61708","O61742","O61792","O61802","O61818","O61848","O61861","O61931","O61955","O62053","O62102","O62183","O62210","O62213","O62244","O62255","O62289","O62305-12","O62327","O62334","O62349","O62388","O62415","O62431","O62481","O62515","O76368","O76371","O76388","O76406","O76449","O76512","O76577","O76606","O76840","P02513","P02566","P02567","P05690","P06125","P06581","P06582","P09446","P0DM41","P11141","P12456","P12844","P12845","P17329","P17331","P18334","P18948-2","P19626","P20163","P27420","P27604","P27639","P27798","P28548","P29691","P30625","P30642","P34255","P34268","P34278","P34286","P34328","P34339","P34342","P34346","P34369","P34378","P34382","P34383","P34385","P34438","P34455","P34460","P34462","P34475","P34496","P34517","P34537","P34540","P34574","P34575","P34659","P34662","P34685","P34689","P34690","P34696","P34697","P34706","P34748","P36573","P37165","P37806-2","P40614","P41932","P41938","P41988","P41996","P42168","P45965","P46502","P46550","P46561","P46562","P46563","P46769","P46822","P47207","P47208","P47209","P47991","P48150","P48152","P48154","P48156","P48158","P48162","P49041","P49180","P49181","P49196","P49197","P49404","P49405","P49632","P50093","P50140","P50432-2","P50880","P51403","P51404","P52015","P52274","P52275","P52713","P52715","P52717","P52819","P52821","P52899","P53013","P53585","P53588","P53596","P54412","P54811","P54812","P55955","P55956","P62784","P90731","P90868","P90916","P90961","P90978","P91020","P91041","P91128","P91175","P91194","P91277","P91283","P91303","P91306","P91349","P91353","P91374","P91398","P91427","P91477","P91844","P91859","P91910","P91913","P91914","P91917","P92020","P92022","P92186-2","P98080","Q03563-2","Q03577","Q04908","Q05036","Q07749","Q07750-1","Q09165","Q09221","Q09236","Q09248","Q09285","Q09289","Q09436","Q09442","Q09444","Q09475","Q09508","Q09509","Q09510","Q09533","Q09543","Q09544","Q09545","Q09583","Q09599","Q09631","Q09665","Q09936","Q09996","Q10033","Q10038","Q10051","Q10129","Q10454","Q10457","Q10657","Q10663","Q11067","Q11174","Q11190","Q17339","Q17348","Q17490","Q17549","Q17561","Q17569","Q17603","Q17624","Q17635","Q17740","Q17763","Q17770","Q17802","Q17832","Q17967","Q17978","Q17994","Q18020","Q18066-2","Q18074","Q18075","Q18115","Q18231","Q18407","Q18421","Q18494","Q18529","Q18577","Q18599","Q18625","Q18680","Q18688","Q18786","Q18787","Q18885","Q18886","Q18905","Q18943","Q18947","Q19007","Q19020","Q19102","Q19126","Q19162","Q19211","Q19240","Q19246","Q19286-2","Q19289-2","Q19324","Q19326","Q19328","Q19339","Q19371","Q19437","Q19478","Q19537","Q19579","Q19591","Q19626","Q19706","Q19746","Q19749","Q19842","Q19869","Q19877","Q19901","Q19969","Q1XFY9","Q20007","Q20025","Q20048","Q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