Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP26.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP39.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP13.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP22.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP35.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP17.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP30.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP18.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP34.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP09.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP21.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP27.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP10.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP23.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP31.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP19.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP14.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP11.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP37.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP15.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP25.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP29.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP38.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/2009_03_23_CAP33.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/Bile_Farina.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/06/PXD000284/Bile_Farina.protXMLprimaryOK200005300Annarita FarinaMass SpectrometryBottom-up proteomicsNot availableHumanOffgelItraqBilehttp://www.ebi.ac.uk/pride/archive/projects/PXD000284Not availablePrideNot availableiodoacetamide derivatized residueiTRAQ4plex-116 reporter+balance reagent acylated residueNot availableProteomicsLTQ OrbitrapPARTIALHomo Sapiens (human)23806607 Farina A, Dumonceau JM, Antinori P, Annessi-Ramseyer I, Frossard JL, Hochstrasser DF, Delhaye M, Lescuyer P; Bile carcinoembryonic cell adhesion molecule 6 (CEAM6) as a biomarker of malignant biliary stenoses., Biochim Biophys Acta, 2013 Jun 24, 10.1016/j.bbapap.2013.06.010annarita.farina@unige.chstructural biology and bioinformaticsDifferentiating malignant from nonmalignant biliary stenoses is challenging. This could be facilitated by the measurement of cancer biomarkers in bile. We aimed at (i) identifying new cancer biomarkers by comparative proteomic analysis of bile collected from patients with a malignant or benign biliary stenosis (exploratory phase) and (ii) verifying the accuracy of the newly identified potential biomarkers for discriminating malignant versus nonmalignant biliary stenoses in a larger group of patients (confirmation phase). Overall, 66 proteins were found overexpressed (ratio>1.5) in at least one cancer condition using proteomic analysis and 7 proteins were increased in all malignant/nonmalignant disease comparisons. Preliminary screening by immunoblot highlighted carcinoembryonic cell adhesion molecule 6 (CEAM6), a cell surface protein overexpressed in many human cancers, as an interesting candidate biomarker. ELISA subsequently confirmed CEAM6 as a potential bile biomarker for distinguishing malignant from benign biliary stenoses with a receiver operating characteristic (ROC) area under the curve (AUC) of 0.92 (specificity 83%, sensitivity 93%, positive predictive value 93%, and negative predictive value 83%). No significant difference in serum CEAM6 level was found between malignant and nonmalignant samples. Combining bile CEAM6 and serum CA19-9 in a panel further improved diagnostic accuracy for malignant stenoses (AUC 0.96, specificity 83%, sensitivity 97%, positive predictive value 93%, and negative predictive value 91%). CEAM6 measurement in bile could be clinically useful to discriminate between malignant and nonmalignant causes of biliary stenosis. This article is part of a Special Issue entitled: Biomarkers: A Proteomic Challenge.Bile carcinoembryonic cell adhesion molecule 6 (CEAM6) as a biomarker of malignant biliary stenoses.Farina Annarita A, Dumonceau Jean-Marc JM, Antinori Paola P, Annessi-Ramseyer Isabelle I, Frossard Jean-Louis JL, Hochstrasser Denis F DF, Delhaye Myriam M, Lescuyer Pierre P530malignant Growth, other disease, Biological Markers, Viral Marker, human being, Surrogate Endpoints, Clinical Markers, Laboratory, Clinical Marker, cell type cancer, Blood, Biochemical, Endpoint, Gene, Serum, supernumerary, cell associated, Surrogate End Points, Surrogate Markers, Laboratory Markers, diseases, Biological, sensitive, Gene Products, disease or disorder, diseases and disorders, neoplasm, sensitivity, malignant tumour, Biomarker, increased, proportion, human disease, Clinical, neoplasm (disease), Biological Marker, proteins, benign, man, CA, non-neoplastic, allergic reaction, Immunologic Markers, Immune, Markers, malignant neoplasm, grupos, Viral Markers, Sludge, Clients, protein binding involved in cell adhesion, Biliary, disorder, Homo sapiens disease, hypersensitivity reaction, Immunologic Marker, Biliary Sludge, Biologic, ratio, malignancy, Viral, grupo, Surrogate Endpoint, Immune Markers., fel, malignant, Serum Markers, Proteins, disorders, End Point, Biochemical Markers, medical condition, Blood Serum, Biologic Marker, Client, stenotic, Immune Marker, group, MT, Marker, Surrogate End Point, malignant neoplasm (disease), Protein, organ system cancer, proteomic analysis, Diseases, condition, Biologic Markers, ELISA, hypersensitivity, primary cancer, Serum Marker, hypersensitive, End Points, Surrogate, stenosis, increased number, Endpoints, proportionality, Rest, rate, Immunologic, cell adhesion molecule, Laboratory Marker, malignant tumor, stricture, Surrogate Marker, human, Protein Gene Products, Gene Proteins, present in greater numbers in organism, disease, Patient, malignant neoplastic disease, Biochemical Marker, gall, hypersensitivity reaction disease, quotient, cancer, cell bound, groupe, Serums, accessory, Gruppe, Immune MarkersNot Available, Unavailable.stenosis., human being, Biliary Sludge, gall, man, Sludge, fel, stenotic, stricture, human, proteomic analysis, Biliarycell adhesion molecule, Biliary Sludge, gall, Sludge, fel, protein binding involved in cell adhesion., BiliaryNot Available, Unavailable.sodium salt, l(4)17, dairy cow, CPD photolyase activity, l(4)13, ammonium formate, Gli, 13C-labeled, cadmium salt, PhrB photolyase activity, ion, Lysine Hydrochloride, Cid[Mel], papular acrodermatitis of childhood, Ci[D], chronic gonococcal infectious disease of lower genitourinary tract., magnesium formate, zinc salt, Solvent, CENP-A, RP11-508D10.1, Long Term, CENP-C, 5730420M11Rik, Polypeptides, KL receptor activity, protein polypeptide chains, Lysine Acetate, Glycoprotein, Drug Tolerance, glycoproteins, cobalt(II) formate dihydrate, PH 3, B1, SCO5, 2, 3, SCO1, 5, NUP96, Software Engineering, 7, outer pigmented layer of retina, 8, glycoproteine, 9, Gsfsow3, rounded, epithelium, WMS, C-Glycosylated Proteins, L Lysine, average, SET, 2810441M03Rik, Divorced, type 5 acid phosphatase, chronic gonococcal infectious disease of upper genitourinary tract, BcDNA:RE21270, F, amino acids, K, M, phosphatase 2A inhibitor I2PP2A, beta-particle, proteins, Divorces, W, HPLC, SUPPRESSOR OF AUXIN RESISTANCE 3, AI047805, DmelCG4299, Bos Tauurus, set, T-cell antigen Gp39, e, pigment epithelium of retina, oxen, decreased, CI, LYS, e-, column, Magics, Lysin, sample, D1, pigmented retina epithelium, CT, Bs, PAC, TNFSF5, Glycosylated Protein, DmAAF34715, drug tolerance, nickel salt, SGS, Neoglycoproteins, CiD, LC-MS2, Ce, PAS, Ci, N-glycanase activity, Crosti-gianotti syndrome, cobalt (+2) salt, N-oligosaccharide glycopeptidase activity, immune system tolerance, Tnfrsf5, Ct, Longterm Effect, Normalcy, collisionally activated dissociation, alpha-amino carboxylic acids, CID, ciD, Cid, Software Tools, hCD40L, ACMICD, (8S-cis)-, PBT, Computer Applications, sodium (4:1:1) salt, Dm1, magnesium salt, Computer Applications Software, dipyrimidine photolyase (photosensitive), IMD3, ci-D, Methionin, jack-bean glycopeptidase, methionine, Hmet, Acid, pbt, Software Applications, HLA-DR-associated protein II, Amino acids, DI-2, CENP-A/Cid, CENP-A/CID, CENPA, Source Software, I-2Dm, formate, NKTL, proportionality, pooled, cromium (+3), rate, alpha, inhibitor of granzyme A-activated DNase, beta Trypsin, GC, human, beta(-), I-2PP1, Applications, TAF-IBETA, Self Tolerance, liquid chromatography-tandem mass spectroscopy, peptide:N-glycanase, ammonium (4:1) salt, acrodermatitis, TAF-Ibeta, Acids, peptide P glycosylase, School-Age Population, bovine, lead (+2) salt, Liquimeth, Ci-155, krk1, Computer Software Applications, l(1)VE614, 2-amino-4-(methylsulfanyl)butanoic acid, C-Glycosylated, nickel formate dihydrate, lead formate, Effects, False, Ly62, TAPK, aluminum salt, e(-), precursor, NAT13P, thomson, protein-containing complex, TR-AP, body system, combined T and B cell immunodeficiency, CG7826, deoxyribodipyrimidine photolyase activity, stratum pigmentosum retinae, mass-to-charge ratio, CenpA, period, LC-MSMS, GP39, CG7835, Gene Products, CG42273, MOD_RES Deamidated asparagine, Neisseria gonorrhoeae, system, Lysine, nat13, Siah, Application, gp39, protein aggregate centre, CD154, Normalities, School-Age Populations, Open Source Softwares, proportion, gonorrhea, anatomical systems, GKLP, CATC4, thallium (+1) salt, Longterm, Software Application, Elektron, glycoproteines, beta-Trypsin, rubidium salt, F23A5.3, Open Source Software, 2pp2a, Long-Term, methanoic acid, Population, ions, Neisseria gonorrhoeae infection, kf, man, Turbo <genus>, O-Glycosylated Proteins, CG10574, Computer Software Application, Injectable, PCTAIRE2-binding protein, MGC:45012, 2PP2A, 2-amino-4-(methylthio)butanoic acid, Tools, glycopeptidase activity, dSET, dSet, CG2125, peptidos, metionina, PTHB1, proto-oncogene c-Kit, NTKL, grupo, cow, mak3, Proteins, ratio., 10-tetrahydro-6, potassium formate, alpha-amino acids, copper (+2) salt, ACP5, L-Isomer, buffer, SPENCDI, Tool, polypeptide, native protein, Immune Tolerance, I-2PP2A, ci[D], Bos primigenius taurus, KIT ligand receptor activity, C18, Acetate, cupric formate, Glycosylated Proteins, chemical analysis, Dm I-2, Long Term Effects, proteomic analysis, MFS1, domestic cattle, aluminum formate, L-Lysine, Met, XKrk1, AU020952, RPE, lithium formate, DmelCG2125, ci155, CenH3, underdeveloped, N-Glycosylated Proteins, Separations, CD117, pI, retinal pigment epithelium, stricture, School Age Populations, beta, Longterm Effects, ME-IV, p. pigmentosa retinae, Computer Programs, Gene Proteins, 12-Naphthacenedione, Applications Softwares, ox, C-Kit, Point, Ssm, glycopeptide N-glycosidase activity, xkl-1, quotient, liquid chromatography tandem mass spectrometry, deoxyribonucleate pyrimidine dimer lyase (photosensitive), TRACP, Mak3, CenpA/CID, Enisyl, MAK3, Immunological Tolerance, glicoproteinas, liquid chromatography tandem mass spectroscopy, IPP2A2, O-Glycosylated, determination, selection process, YM 3, Aminosaeure, zinc formate, cleavage, Amino acid, atado, lead salt, Xkl-1, protein, combined T cell and B cell immunodeficiency, pigmented epithelium, peptide, Cut, School-Age, Tnfsf5, L-Isomer Methionine, Gsfsco1, ammonium tetraformate, Methionine, peptido, ClvPrd, l(1)7Ba, l(1)7Bb, Min, protein aggregate, present in fewer numbers in organism, Gsfsco5, Injectables, Effect, Computer Program, DmelCG42273, SOW3, CG13329, T-BAM, glicoproteina, Glykoprotein, N-Glycosylated, LCMSMS, tartrate-resistant acid ATPase, PNGase F, kDa, peptides, TAF-I, Open, Bos bovis, hypoplasia, pigmented retina, min, PNGase A, Computer Programs and Programming, epsilon-diaminocaproic acid, mAPC, CG12352, Isoelectric, copper, CG11387, DNA cyclobutane dipyrimidine photolyase activity, hNAT5, IGAAD, TRAP3, lithium salt, Tudor repeat associator with PCTAIRE-2, hSAN, DmelCG10574, Sludge, ppm, Cenp-A, domestic cow, TEAB, T5ap, Sl, MGC - 45012, GPHYSD2, Biliary Sludge, Racemethionine, ammonium (2:1) salt, Long-Term Effects, Mak3p, CD40L, phapii, PRE, NAT13, Aminokarbonsaeure, deoxyribonucleic cyclobutane dipyrimidine photolyase activity, LC-MS/MS, Cd40l, 2-Amino-4-(methylthio)butyric acid, Dmel_CG7826, StF-IT-1, Tr-kit, Th, infections, pigmented retinal epithelium, Nat13, stenotic, Source Softwares, Isoelectric Points, Programs, Program, N-linked-glycopeptide-(N-acetyl-beta-D-glucosaminyl)-L-asparagine amidohydrolase activity, HCD, retinal pigment, Ionen, School Age, Computer Applications Softwares, lysine, kl1-A, Immunologic Tolerance, TNF-related activation protein, Softwares, BcDNA:GH10590, KIT, nickel (+2) salt, Ly-62, fixed, retinal pigment layer, Dmel_CG7835, cenpA, Mnb, MNB, parent ion, tyrosine-protein kinase Kit, Amino Acid, Turbo <subgenus>, ammonium salt, Populations, Ci/Gli, Ci/GLI, CenH3/CID, stenosis, retinal pigmented epithelium, Glycosylated, cobaltous formate, AW743063, kit, CG4299, MASS, AW124434, AI326936, organ system, congenital combined immunodeficiency, phr A photolyase activity, Health, DNA-photoreactivating enzyme, precursor ion, Tolerance, Patient, TrATPase, copper salt, IGM, CD40LG, Polypeptide, i2pp2a, 2600005K24Rik, time, Gruppe, nat5, human being, TRAP, p50, cesium salt, Aminocarbonsaeure, SCF receptor activity, L Isomer, FBN, Gene, NAT5P, electron, Computer, alpha-amino acid, photoreactivating enzyme activity, Gianotti Crosti syndrome, PHAPII, LC-MS-MS, Buffer, formic acid, 11-trihydroxy-8-(hydroxyacetyl)-1-methoxy-10-((2, l(4)102ABc, papular infantile, potassium salt, reduced, scfr, template-activating factor I, polypeptide chain, cattle, subnumerary, ECTOL1, Injection, 14C-labeled, L-Methionine, stratum pigmentosa retinae, tiny, CD40-L, PNGase, SCFR, Separated, Pedameth, CenH3[Cid], CenH3[CID], Fdc, MOS3, deoxyribocyclobutadipyrimidine pyrimidine-lyase activity, PRECOCIOUS, iones, ipp2a2, DmelCG13329, decreased number, calcium formate, OCTD, X-linked combined immunodeficiency, DmelCG11387, 10^[-6], TEIF, grupos, taf-ibeta, Trap, Clients, DmelCG12352, Biliary, Nat5, NAT5, HIGM1, cromium (+3) salt, Long-Term Effect, School Age Population, F23A5_3, sodium formate, C78062, Glykoproteine, span, combined immunodeficiency, small, Applications Software, P105, Open Source, DYRK1, data, Computer Software, nickel formate, chronic gonococcal infectious disease of lower genitourinary tract, DL-Methionine, fel, protein complex, PCTAIRE2BP, igaad, YM3 doxorubicin analog, YM-3, strontium formate, cenH3, Bp50, infantile lichenoid, strontium salt, Client, Peptide, group, AW112078, stratum pigmentosum (retina), LC/MS/MS, MODIFIER OF SNC1, Software Tool, Oligosaccharide, CAD, natural protein, Ci155, Protein, deoxyribonucleic photolyase activity, I2PP2A, 6-trideoxy-3-((3-pyridinylmethyl)amino)-alpha-L-arabino-hexopyranosyl)oxy)-, Dyrk1, Vacuums, connected anatomical system, Software, WMS2, Data Base, San, SAN, Separation, c-KIT, negatron, photolyase activity, Normality, CC1, Engineering, Rest, Tripcellim, Points, Amino, sample population, chromic formate, incised, Protein Gene Products, san, Trypure, dSET/TAF-Ibeta, c-kit, 2610030F17Rik, Ion, gall, SSKS, Deamidated asparagine, calcium salt, alpha-amino-gamma-methylmercaptobutyric acid, Peptid, round, assay, variable, AA407739, 6-diaminohexanoic acid, groupe, CID/CENP-A0trueComparative proteomic analysis of human bile from malignant and nonmalignant biliary stenosisFour bile samples collected from patients with a malignant (PAC, CC) or a nonmalignant (CP, BS) biliary stenosis were used for comparative proteomic analysis. Briefly, bile samples were centrifuged at 16,000/g/ for 10 min at 4 C. Each supernatant was delipided with Cleanascite (Biotech Support Group, North Brunswick, NJ, USA) and ultrafiltrated using a 3 kDa filter cut-off (YM-3 centricon, Millipore, Bedford, MA, USA). For each sample, 50 ug of proteins were mixed with 0.5 M triethylammonium bicarbonate (TEAB) buffer pH 8.0 to a total volume of 100 uL. One ug of bovine lactoglobulin (LACB) was spiked in each sample as an internal standard. Proteins were reduced and alkylated before digestion of N-linked oligosaccharides from glycoproteins using PNGase F (Sigma-Aldrich, St. Louis, MO, USA). Proteins were then digested with trypsin and the resulting peptides were tagged with one of the isobaric tags from the iTRAQ reagents 4plex kit (AB Sciex, Foster City, CA, USA) according to the manufacturer's instructions. The four samples were then pooled and dried under vacuum. Labeled peptides were fractionated according to their isoelectric point on an Agilent 3100 OFFGEL fractionator using 24 cm pH 3-10 linear immobilized pH gradients (GE Healthcare, Chalfont St. Giles, UK) following manufacturer's instructions. Fractions were then recovered in separate tubes for high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). MS analysis was performed on a LTQ Orbitrap XL from Thermo Electron (San Jose, CA, USA) equipped with a NanoAcquity HPLC system from Waters. Peptides were trapped on a home-made 5 um 200 A Magic C18 AQ (Michrom, Auburn, CA, USA) 0.1 x 20 mm pre-column and separated on a home-made 5 um 100 A Magic C18 AQ (Michrom) 0.75 x 150 mm column with a gravity-pulled emitter. The analytical separation was run for 65 min using a gradient of H_2 O/formic acid (FA) 99.9%/0.1% (solvent A) and CH_3 CN/FA 99.9%/0.1% (solvent B). The gradient was run at a flow rate of 220 nL/min as follows: 5% B for 1 min, from 5 to 35% B in 54 min, from 35 to 80% B in 10 min. For MS survey scans, the OT resolution was set to 60,000 and the ion population was set to 5 x 10^5 with an m/z window from 400 to 2000. Maximum of 3 precursors were selected for both collision-induced dissociation (CID) in the LTQ and high-energy C-trap dissociation (HCD) with analysis in the Orbitrap (OT). For MS/MS in the LTQ, the ion population was set to 1 x 10^4 (isolation width of 2 m/z) while for MS/MS detection in the OT, it was set to 2 x 10^5 , with resolution of 7,500, first mass at m/z = 100, and maximum injection time of 750 ms. The normalized collision energies were set to 35% for CID and 60% for HCD. Peak lists from MS analysis were searched against Uniprot_Swissprot database (version 2011_2) using Phenyx protein identification software (GeneBio, Geneva, Switzerland). /Homo Sapiens/ taxonomy was specified for database searching. The parent ion tolerance was set to 10 ppm. Variable amino acid modifications were oxidized methionine and deamidated asparagine. Carbamidomethylation of cysteines and iTRAQ-labeled peptides on the amino terminus and lysine were set as fixed modifications. Trypsin was selected as the enzyme, with one potential missed cleavage, and the normal cleavage mode was used. Only one search round was used with selection of "turbo" scoring. The peptide p value was 10^-2 for LTQ-OT data. Protein and peptide scores were set up to maintain the false positive rate below 5%.2013-06-202013-06-11PXD00028444544641524991755931173880433309286643235983906288705327160246197160329358113635894532758385118794713512251177505855571468353649215891030629159364151584938858033271591002263888691513558814647969287713328038365458333985808946211825906329571749341247272563227321928672813954513294914081691390363453011431140981087135547714245073264435551274432714694559729375146157546478422422089643174471270139011431933829355082307411148119466948969352937251264690727580240216599366581216595294128128329654312821280339523218184318329204353152281125755478757593079725362311436733115955644421432138184922242619109697622615515311179573347986588845586265281736309431796989361604885664162425678253175136303555432046565050632633NCBITaxon:13134565860688607699389427955453512014887795921612941002642032890969456967631076228531643518543121574577760494144242324113NCBITaxon:177392979310239101178709NCBITaxon:4055974990799136526112273498217170187367451011656915693132842610360983341336795663303NCBITaxon:4932992588391000589873299404458611535722491134305311563120175544219329935872631787236669872464268727547624223141461151041607913055468911180066125522856213616299767982510216998231174673116761080772326424446896776744685960039376511822635735784588086363266689NCBITaxon:26970491031298382413681089319975961231974794652283291761966277668960596061572951372211711012567371233435105231100904054998638783104298635788401937334961519821597397574878229719721519089850764802563184189856239190264946454635286746360NCBITaxon:110712883574612874601134506649125314594352638NCBITaxon:615797787237953440812633803945264114595361235178616722724323074691123869978540979541NCBITaxon:29544243277627912924970361251802848124264281504752697961029938865990346941734751541554036329979662877323962899548657558462823823522862871736231399463601068842043601043994796858355847954440426071016780781440772NCBITaxon:61918402317199312811836100361050231855791908021878783749907038476641692259634591065922607046183260705334542260707NCBITaxon:780NCBITaxon:150245151688531870292115869103209285747078124966842528619212638541094343197383379192875NCBITaxon:10359282912680639560295987044830064180224275210555242354432243081433614690081502575412983964271191364516996328104121697927262327262430085212389936436801097791356223347471821314269704958331361858030826882976052779638185833424090611477871274414153681639588858130767494022432640817212816140559204254687613837081274423285099011912744201274426519534911370249240543734131641660817562615282352567506028532189517731193501846451450511492412153239564818822970915800257125614801549031384736031423166385962859631015101450520493227591428693732664179332648373153NCBITaxon:9615297221097677112111449376083334191107927628333223756126662555592924847914869177492003600943407335247225944751750375451649908515151438992374723806607