Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001133/W303-1A_Mutant_Batch.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001133/W303-1A_Wt_Colony.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001133/W303-1A_Mutant_Colony.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001133/W303-1A_Wt_Batch.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001133/LIQ13-86_FabioFariaSPSaccarocere2miss.prot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001133/WT1SPSACCHAROMYCES.prot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001133/AmiSOL13-86_FabioFariaSPSaccarocere2miss.prot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001133/WTML12171SPSACCHARORBITRAP.prot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001133/W303-1A_Mutant_Colony.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001133/W303-1A_Mutant_Batch.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001133/W303-1A_Wt_Colony.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001133/W303-1A_Wt_Batch.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001133/W303-1A_Mutant_Colony.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001133/W303-1A_Mutant_Batch.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001133/W303-1A_Wt_Colony.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001133/W303-1A_Wt_Batch.mgfprimaryOK200004100clucas@bio.uminho.ptFábio Faria-OliveiraMass SpectrometryShotgun proteomicsNot availableYeastYeast; ecm; colony; multicellular agregatescolonymulticellular agregatesecmhttp://www.ebi.ac.uk/pride/archive/projects/PXD001133The development of a uniform ±3 mm-thick homogenous yeast culture mat was obtained spreading evenly 1.5 ml of YPD batch cultures at OD600=1 in Ø 90 mm YPDa plates, and incubating for 7 days at 30 °C. The cellular biomass of 200 plates was gently transferred into 50 ml Falcon tubes. The suspension was washed with PBS buffer (NaCl 100 mM; KCl 2.7 mM; Na2HPO4.2H2O 10 mM; KH2PO4 20 mM; pH 7.4), supplemented with a protease inhibitor cocktail (PMSF 0.2 µg/ml; Aprotinin 0.32 µg/ml; Pepstatin 1 µg/ml; Leupeptin 1 µg/ml), and incubated for 10 min with constant rotation in a tube roller SRT1 (Stuart, Staffordshire, UK). The suspension, containing cells and ECM, was spun down for 10 min at 15,000 rpm and 4 °C in a Sigma 4-16K centrifuge (Sigma, Osterode, Germany). The supernatant was collected, filtered and freeze-dried. The proteins were precipitated using the chloroform/methanol protocol. Overnight batch cultures on liquid YPD with an air:liquid ratio of 2:1 were used as control, to assess the proteins secreted to the extracellular growth medium. These cultures were centrifuged for 10 min at 5,000 rpm, and the supernatants collected and processed in the same way as the ECM samples. Total protein extract (50 µg) was brought up to 40 µl with MilliQ water and mixed with loading buffer 5X. The samples were loaded and run at low voltage (25 V) until the migration front reached 2-3 mm above the resolving gel. The bands containing protein total extract were excised and digested overnight at 37 °C with sequencing grade trypsin (Roche Biochemicals) in 25 mM ammonium bicarbonate (pH 85), 1 µg/20µg protein. After digestion, the supernatant from the excised protein bands were analysed by LC-MS/MS.PrideNot availableiodoacetamide derivatized residuemonohydroxylated residueThe total extract samples (5 μl), in 0.1% formic acid for a final concentration of 1 μg/μl were loaded onto a C18-A1 ASY-Column 2 cm pre-column (Thermo Scientific) and then eluted onto a Biosphere C18 column (inner diameter 75 µm, 15 cm long, 3 µm particle size) (NanoSeparations). The proteins were separated using a gradient on a nanoEasy HPLC (Proxeon), coupled to a nanoelectrospray ion source (Proxeon), at a flow-rate of 250 nl/min. The mobile phase A consisted of 0.1% formic acid in 2% ACN, and mobile phase B was 0.1% formic acid in 100% ACN. A solvent gradient was applied for 140 min, from 0% to 35% phase B. Mass spectra were acquired on the LTQ-Orbitrap Velos (ThermoScientific) in the positive ion mode. Full-scan MS spectra (m/z 400-1,800) were acquired with a target value of 1,000,000 at a resolution of 30,000 at m/z 400 and the 15 most intense ions were selected for collision induced dissociation (CID) fragmentation in the LTQ with a target value of 10,000 and normalized collision energy of 38%. Precursor ion charge state screening, and monoisotopic precursor ion selection, were enabled. Singly charged ions and unassigned charge states were rejected. Dynamic exclusion was enabled with a repeat count of 1 and exclusion duration of 30 ms. Proteome Discoverer 1.2 with MASCOT 2.3 was used as search engine to search in the Uniprot/Swissprot (taxonomy Saccharomyces cerevisiae) database (7,798 sequences). The search parameters used were the following: peptide tolerance - 10 ppm; fragment ion tolerance – 0.8 Da; missed cleavage sites – 2; fixed modification - carbamidomethyl cysteine, and variable modifications - methionine oxidation. Mascot ion score 20 and a 99% peptide confidence were set as filters.ProteomicsCândida Manuel Ribeiro Simões LucasLTQ Orbitrap VelosCBMA - Univeristy of MinhoPARTIALSaccharomyces Cerevisiae (baker's Yeast)ffariaoliveira@bio.uminho.pt26608260 Faria-Oliveira F, Carvalho J, Ferreira C, Hernáez ML, Gil C, Lucas C. Quantitative differential proteomics of yeast extracellular matrix: there is more to it than meets the eye. BMC Microbiol. 2015 Nov 25;15(1):271 10.1186/s12866-015-0550-1BiologicalUniversity of Minho<h4>Background</h4>Saccharomyces cerevisiae multicellular communities are sustained by a scaffolding extracellular matrix, which provides spatial organization, and nutrient and water availability, and ensures group survival. According to this tissue-like biology, the yeast extracellular matrix (yECM) is analogous to the higher Eukaryotes counterpart for its polysaccharide and proteinaceous nature. Few works focused on yeast biofilms, identifying the flocculin Flo11 and several members of the HSP70 in the extracellular space. Molecular composition of the yECM, is therefore mostly unknown. The homologue of yeast Gup1 protein in high Eukaryotes (HHATL) acts as a regulator of Hedgehog signal secretion, therefore interfering in morphogenesis and cell-cell communication through the ECM, which mediates but is also regulated by this signalling pathway. In yeast, the deletion of GUP1 was associated with a vast number of diverse phenotypes including the cellular differentiation that accompanies biofilm formation.<h4>Methods</h4>S. cerevisiae W303-1A wt strain and gup1∆ mutant were used as previously described to generate biofilm-like mats in YPDa from which the yECM proteome was extracted. The proteome from extracellular medium from batch liquid growing cultures was used as control for yECM-only secreted proteins. Proteins were separated by SDS-PAGE and 2DE. Identification was performed by HPLC, LC-MS/MS and MALDI-TOF/TOF. The protein expression comparison between the two strains was done by DIGE, and analysed by DeCyder Extended Data Analysis that included Principal Component Analysis and Hierarchical Cluster Analysis.<h4>Results</h4>The proteome of S. cerevisiae yECM from biofilm-like mats was purified and analysed by Nano LC-MS/MS, 2D Difference Gel Electrophoresis (DIGE), and MALDI-TOF/TOF. Two strains were compared, wild type and the mutant defective in GUP1. As controls for the identification of the yECM-only proteins, the proteome from liquid batch cultures was also identified. Proteins were grouped into distinct functional classes, mostly Metabolism, Protein Fate/Remodelling and Cell Rescue and Defence mechanisms, standing out the presence of heat shock chaperones, metalloproteinases, broad signalling cross-talkers and other putative signalling proteins. The data has been deposited to the ProteomeXchange with identifier PXD001133.<h4>Conclusions</h4>yECM, as the mammalian counterpart, emerges as highly proteinaceous. As in higher Eukaryotes ECM, numerous proteins that could allow dynamic remodelling, and signalling events to occur in/and via yECM were identified. Importantly, large sets of enzymes encompassing full antagonistic metabolic pathways, suggest that mats develop into two metabolically distinct populations, suggesting that either extensive moonlighting or actual metabolism occurs extracellularly. The gup1∆ showed abnormally loose ECM texture. Accordingly, the correspondent differences in proteome unveiled acetic and citric acid producing enzymes as putative players in structural integrity maintenance.Quantitative differential proteomics of yeast extracellular matrix: there is more to it than meets the eye.Faria-Oliveira Fábio F, Carvalho Joana J, Ferreira Célia C, Hernáez Maria Luisa ML, Gil Concha C, Lucas Cândida CBovine Kunitz Pancreatic Trypsin Inhibitor, Methane, liquid chromatography tandem mass spectroscopy, single-organism developmental process, muriate of potash, YPD, KCl, protein, apg, FabD, extracted material, trichloro-, Antilysin, Background, protein polypeptide chains, halite, Trypsin Inhibitor, Kontrykal, Traskolan, Klotrix, Cultural, P-mad, Kontrikal, Min, L-Leucinamide, protein aggregate, Smad, sylvite, DmelCG42273, wood alcohol, ethnicity, me75, LCMSMS, malonyl-CoA:ACP transacylase activity, reference sample, P-Mad, yeast extract peptone dextrose, SAMS, min, mAPC, proteins, D17Mit170, T1, T2, AI047805, cloruro sodico, dMAD, dMad, salt, GPIa*, N-acetyl-L-leucyl-N-(4-((aminoiminomethyl)amino)-1-formylbutyl)-, leupeptin, anatomical tube, p-Mad, Rotations, Sodium Methoxide, Iniprol, malonyl transferase activity, ratio, LC-MS2, [KCl], Tube, rock salt, Klor-con, malonyl-CoA:AcpM transacylase activity, Alcohol, SAMS1, LC-MS/MS, Dmel_CG7826, Kunitz, Tl3, Bovine Pancreatic Trypsin Inhibitor, Tl2, TUBE, Cultural Background, common salt, batch, Cultures, Dm1, THIL, Kaon-Cl 10, ahpatinin C, Pancreatic, Carbinol, Dmel_CG7835, Hydrogen Oxide, carbinol, Mnb, MNB, Clinorotations, Yeast, PMad, proportionality, En(vvl), rate, AI046368, AW124434, natrii chloridum, study protocol, beta Trypsin, pSmad, CG12399, Trichloromethane, Clinorotation, METHANOL, Wood, rotation, spirit of wood, liquid chromatography-tandem mass spectroscopy, duct, pMAD, pMad, Monopotassium chloride, acyl-carrier-protein S-malonyltransferase activity, Methyl Alcohol, Beliefs, MATA1, malonyl coenzyme A-acyl carrier protein transacylase activity, Gene, Methyl, CH3OH, [acyl-carrier protein] S-malonyltransferase activity, ACP S-malonyltransferase activity, acetyl-L-leucyl-L-leucyl-L-arginal, protein-containing complex, Methylalkohol, CG7826, Buffer, method, 2/23, polypeptide chain, malonyl transacylase activity, Pulmin, method used in an experiment, LC-MSMS, AdoMet, CG7835, Gene Products, MAD, CG42273, Kallikrein-Trypsin, Wood Alcohol, Low, Backgrounds, MAT, Mat, proportion, 2310061K05Rik, malonyl-CoA:acyl-carrier-protein S-malonyltransferase activity, uniform, mad, beta-Trypsin, chlorure de sodium, Cultural Relativisms, methanol, BPTI, Basic Pancreatic Trypsin Inhibitor, E(zen)2, (S)-isomer, Kallikrein-Trypsin Inactivator, l(2)k00237, Suspension, monoacetate, wood naphtha, ECM, Kochsalz, Smad1, Customs, LC-MS-MS., liquid, culture, Kallikrein Trypsin Inactivator, Zymofren, Controlled, constant, Inactivator, DYRK1, Controlling, cou, potassium chloride, protein complex, acyl carrier protein malonyltransferase activity, malonyl-CoA:acyl carrier protein transacylase activity, Proteins, Dilmintal, Methyl alcohol, Cultural Backgrounds, buffer, Cell, EMILIN4, Methanol, Belief, Kaliumchlorid, development, PBS buffer, LC/MS/MS, Ams, Lr, native protein, acyl carrier proteinmalonyltransferase activity, Natriumchlorid, natural protein, Protein, AI551343, table salt, Sodium, Dyrk1, MeOH, NaCl, dJ142L7.2, Basic, AU020952, Contrical, ACAT, CC1, Tripcellim, ME-IV, Protein Gene Products, plan specification, Gene Proteins, extracellular, Trypure, l(2)K00237, DmelCG12399, c28, malonyl-CoA-acyl carrier protein transacylase activity, Biomasses, MCAT activity, wood spirit, Kunitz Pancreatic Trypsin Inhibitor, quotient, Bra, Relativisms, liquid chromatography tandem mass spectrometry, Contrykal, Relativism, MMRN, Cultural Relativism, pepstatin A, Trasylol, Methoxide, sodium chloridelight-detecting organ, eye globe, Yeast, matrisome, regio orbitalis, Peptidomics, eyes, visual system, number, Matrix, bulbus oculi, vertebrate eye, Matrices, presence, visual apparatus., Eyes, count in organism, camera-type eye plus associated structures, orbital region, orbital part of face, Extracellular, eye, globe, quantitative, Extracellular Matrices, proteinaceous extracellular matrix, eyeball, presence or absence in organismsodium salt, l(4)17, CPD photolyase activity, l(4)13, IPP2A2, ammonium formate, Gli, 13C-labeled, Size, cadmium salt, AA407876, PhrB photolyase activity, ion, selection process, Cid[Mel], Ci[D], Particle, zinc formate, cleavage, lead salt, magnesium formate, protein, combined T cell and B cell immunodeficiency, pigmented epithelium, extracted material, zinc salt, Solvent, CENP-A, mKIAA4153, CENP-C, 5730420M11Rik, peptide, Polypeptides, AA409940, protein polypeptide chains, L-Isomer Methionine, ammonium tetraformate, Drug Tolerance, Methionine, ClvPrd, peptido, BANF, cobalt(II) formate dihydrate, AA960376, B1, Nbla10545, 3, Min, NUP96, outer pigmented layer of retina, protein aggregate, 1110020G17Rik, epithelium, WMS, DmelCG42273, C79691, CG13329, SET, Divorced, BcDNA:RE21270, C79325, i2pp2a., NOGO-A, peptides, TAF-I, NOGO, M, long, 2610036I19Rik, phosphatase 2A inhibitor I2PP2A, 2610510L13Rik, pigmented retina, min, mAPC, proteins, Divorces, Baker, copper, Saccharomyces uvarum var. melibiosus, SCAN, DNA cyclobutane dipyrimidine photolyase activity, HPLC, SUPPRESSOR OF AUXIN RESISTANCE 3, AI047805, Lccp, DmelCG4299, set, IGAAD, fSAP152, pigment epithelium of retina, lithium salt, CI, ASY, column, DmelCG10574, Saccharomyces capensis, ACN, ppm, D1, Acn, Cenp-A, pigmented retina epithelium, Baker's, Mycoderma cerevisiae, Half Cystine, brewer's yeast, GPHYSD2, z, Racemethionine, ammonium (2:1) salt, drug tolerance, nickel salt, SGS, ratio, CiD, phapii, PRE, Ce, Ci, NI220/250, NOGOC, cobalt (+2) salt, Zinc Cysteinate, immune system tolerance, deoxyribonucleic cyclobutane dipyrimidine photolyase activity, 2-Amino-4-(methylthio)butyric acid, Dmel_CG7826, StF-IT-1, Th, Search, Nogo-B, Nogo-C, pigmented retinal epithelium, Nogo-A, Thermo Scientific, not genetically inherited, collisionally activated dissociation, CID, ciD, Cid, ACMICD, Baker's Yeasts, sodium (4:1:1) salt, count, magnesium salt, retinal pigment, Dm1, Ionen, Saccharomyces diastaticus, dipyrimidine photolyase (photosensitive), Sizes, Immunologic Tolerance, ci-D, Methionin, methionine, Hmet, nickel (+2) salt, retinal pigment layer, Dmel_CG7835, cenpA, Mnb, MNB, parent ion, ammonium salt, Yeast, HLA-DR-associated protein II, Ci/Gli, Ci/GLI, CenH3/CID, CENP-A/Cid, CENP-A/CID, DI-2, retinal pigmented epithelium, CENPA, I-2Dm, formate, proportionality, cobaltous formate, cromium (+3), rate, MASCOT, L-Cysteine, MASS, CG4299, inhibitor of granzyme A-activated DNase, AW124434, congenital combined immunodeficiency, I-2PP1, nanospray, phr A photolyase activity, DNA-photoreactivating enzyme, Self Tolerance, TAF-IBETA, precursor ion, Tolerance, copper salt, ammonium (4:1) salt, TAF-Ibeta, Polypeptide, lead (+2) salt, Liquimeth, Ci-155, time, Brewer's Yeast, Particle Sizes, Cysteine Hydrochloride, 2-amino-4-(methylsulfanyl)butanoic acid, nickel formate dihydrate, lead formate, AW549739, aluminum salt, cesium salt, NI220|250, Brewer's, L Isomer, FBN, Gene, baker's yeast, ACINUS, precursor, photoreactivating enzyme activity, protein-containing complex, thomson, combined T and B cell immunodeficiency, Saccharomyces italicus, deoxyribodipyrimidine photolyase activity, CG7826, PHAPII, stratum pigmentosum retinae, formic acid, mass-to-charge ratio, CenpA, period, l(4)102ABc, potassium salt, mKIAA0886, polypeptide chain, template-activating factor I, NSP, ECTOL1, 14C-labeled, C130026I10Rik, Gene Products, CG7835, CG42273, L-Methionine, stratum pigmentosa retinae, Half-Cystine, Separated, Siah, Pedameth, CenH3[Cid], CenH3[CID], Search Engines, proportion, MOS3, deoxyribocyclobutadipyrimidine pyrimidine-lyase activity, PRECOCIOUS, Acinus, thallium (+1) salt, iones, L Cysteine, ipp2a2, rubidium salt, F23A5.3, DmelCG13329, 2pp2a, calcium formate, methanoic acid, ions, CG10574, S. cerevisiae, acinusL, OCTD, X-linked combined immunodeficiency, mKIAA0989, S cerevisiae, Candida robusta, 2-amino-4-(methylthio)butanoic acid, 2PP2A, 10^[-6], acinusS, taf-ibeta, dSET, dSet, CG2125, peptidos, cromium (+3) salt, metionina, sodium formate, F23A5_3, PTHB1, combined immunodeficiency, DYRK1, nickel formate, NgA, Saccharomyces cerevisiae 'var. diastaticus', DL-Methionine, Nbla00271, protein complex, Proteins, igaad, strontium formate, cenH3, potassium formate, copper (+2) salt, strontium salt, L-Isomer, NSP-CL, mKIAA0670, Peptide, Engine, polypeptide, stratum pigmentosum (retina), MODIFIER OF SNC1, CAD, native protein, Immune Tolerance, natural protein, ci[D], I-2PP2A, C18, Ci155, cupric formate, RTN4-A, Protein, Dm I-2, RTN4-C, deoxyribonucleic photolyase activity, I2PP2A, MFS1, Dyrk1, aluminum formate, RTN-X, WMS2, Data Base, Met, Saccharomyces oviformis, AU020952, Separation, lithium formate, RPE, DmelCG2125, ci155, CenH3, photolyase activity, RTN4-B2, RTN4-B1, Separations, CC1, retinal pigment epithelium, chromic formate, p. pigmentosa retinae, ME-IV, Protein Gene Products, Gene Proteins, dSET/TAF-Ibeta, 2610030F17Rik, Ion, concentration, SSKS, calcium salt, quotient, alpha-amino-gamma-methylmercaptobutyric acid, Peptid, deoxyribonucleate pyrimidine dimer lyase (photosensitive), Baker's Yeast, variable, SCH, AA407739, CenpA/CID, Baker Yeast, CID/CENP-A, Immunological ToleranceSaccharomyces oviformis, Yeast, matrisome, Saccharomyces cerevisiae 'var. diastaticus', single-organism developmental process., Brewer's, Matrix, baker's yeast, Baker, Matrices, Saccharomyces uvarum var. melibiosus, Saccharomyces italicus, S. cerevisiae, Baker's Yeasts, development, S cerevisiae, Candida robusta, Saccharomyces capensis, Saccharomyces diastaticus, Extracellular, MSR, proteomic analysis, Baker's, Mycoderma cerevisiae, Extracellular Matrices, Baker's Yeast, brewer's yeast, Brewer's Yeast, proteinaceous extracellular matrix, Baker YeastATHSP70, Intercellular, H3cit, l(3)L3929, anon-WO0118547.303, FEZL, Metabolic Concepts, signal secretion, Hsc-4, morphogenesis, scd, Space, Cell Interactions, dmTAF[[II]]230, protein polypeptide chains, Communications, Method, AA959943, 2, Cell-to-Cell, Analysis, Extracellular Matrices, Metabolism Concept, myd, ASD2, irp94, DmelCG6489, DmelCG18743, hsp70 Ba, Divorced, TFIID TAF250, SDS-PAGE electrophoresis, Analyses, cel, catabolism, FATE, Tissue, HSPH2, HS24/P52, Mbp-1, composition, proteins, Divorces, metabolic process resulting in cell growth, procedures, number of, Eucarya, Saccharomyces uvarum var. melibiosus, ecotype, Hsp70Bc, HPLC, Hsp70Bb, hsp70 Aa, Intercellular Space, Hsp70Bd, GPIa*, Glycan, n, Mycoderma cerevisiae, DMob1, 5-dioic acid, hsc70, single organism signaling, Acta-2, LC-MS2, Hsp70b, Dmob1, dTAF[[II]]230, Hsp70a, Citrate, functional failure, metabolism resulting in cell growth, TAF200, Actsk-1, Procedure, HS24|P52, signal transduction by trans-phosphorylation, HSP70Bbb, Glykane, Hsp70B, Baker's Yeasts, Hsp70A, Interaction, Saccharomyces diastaticus, Dm-hsp70, mKIAA0609, secretion, simple tissue, ARABIDOPSIS HEAT SHOCK PROTEIN 70, Hydrogen Oxide, fg, DmelCG4264, Cell Communications, content, Anhydrous Citric Acid, Eukarya, expanded, Methodological, hsp-70, Phenotypes, MDC1D, signaling pathway, enr, Taf250, liquid chromatography-tandem mass spectroscopy, Hsp70A7d, dhsp70, HSC70-4, CG31449, Brewer's Yeast, Intercellular Spaces, TAF230, CG5834, l(3)j7A4, big, xshh, atypia, Processes, Biocatalysts, number, hs70, Metabolic Processes, protein-containing complex, SDS polyacrylamide gel electrophoresis of proteins, Saccharomyces italicus, bs17d06.y1, 87A, CITRIC ACID, Gup, large, TOF, hsp70RY, LC-MSMS, DMHSP7A2, Gene Products, hedgehog, atypical, Technique, hsp70Bb-prime, Polysaccharide, ZNF312, dTAF[[II]]250, Cell Interaction, DMHSP7D1, cell, MDDGB6, BAP74, nutrients, Hsp 70, Cell-to-Cell Interaction, Metabolic Concept, CG31359, Hsc70, S. cerevisiae, CG31354, Study, dTAF250, S cerevisiae, Enzyme, embryogenesis and morphogenesis, HSC70, Extracellular, Citric acid, 2-Hydroxytricarballylic acid, Hsp-70Aa, Mob1, Hsc4p, Anhydrous, CG31366, grupo, Eukaryote, degradation, Proteins, BG:DS00004.13, defective, compositionality, Hsp70ab, Cell, EMILIN4, eukaryotes, dTAF230, Concept, hsp70A, E(csp)1545, hsp70B, native protein, hsc70-4, Strain, TAF[[II]]250/230, background, signalling cascade, metabolism, CG13852, Metabolic Phenomenon, Saccharomyces oviformis, Taf[[II]]250, polisacaridos, hsp70a, hsp70b, dhsp70Aa, multicellular organism metabolic process, l(3)03550, i190, Separations, sodium dodecyl sulphate–polyacrylamide gel electrophoresis, 3-propanetricarboxylic acid, signalling, extracellular, Gene Proteins, ZFP312, DmHSP70AA, signalling process, structure, liquid chromatography tandem mass spectrometry, DmelCG31449, Eucaryotae, quantitative, Baker's Yeast, Hsp70Bbc, Interactions, HSP70Bc, Data Analyses, Hsp70aB, Anabolism, liquid chromatography tandem mass spectroscopy, biochemical pathways, Metabolic Process, Hsp70, Mbp1, Glykan, protein, heat shock protein 70, Principal Component Analyses, composed of, dmob1, HSP70, polisacarido, APG-2, Techniques, Hsp110, Concepts, protein aggregate, Phenomenon, signal transduction by protein phosphorylation, portion of tissue, RY, LCMSMS, DmelCG13852, reference sample, enzymes, Glycans, availability, DmelCG5834, TACHD, hsc4, Cell to Cell Interaction, xhsp70, Glycane, Baker, DmelCG31359, 2-Hydroxy-1, Extracellular Spaces, eucaryotes, Acts, Communication, GRP78, Methodological Studies, Saccharomyces capensis, signaling process, Baker's, biotransformation, DmelCG31366, CG4264, Strains, brewer's yeast, associated, Catabolism, wide/broad, organisation, aberrant, Process, dHsp70, gyltl1b-b, Citric Acid Monohydrate, LC-MS/MS, signal transduction by conformational transition, 2-hydroxypropane-1, extra or missing physical or functional parts, TAFII-250, TAF250/230, results, Citric Acid, signaling cascade, TAFII250, batch, MDDGA6, CT43, tissue portion, cultivar, failure, Biofilm, regulator, HSC4, Hsc4, Yeast, gyltl1b, Hsp70-Ab, Hsp70-BC, mdc1d, SDS polyacrylamide gel electrophoresis, Maintenances., CG17603, TAF[[II]], Methodological Study, polysaccharides, wide, Principal Component, enlarged, SR3-5, Biocatalyst, CG6489, 3-tricarboxylic acid, Strains and Sprains, Proteomes, Gruppe, d230, biological signaling, Procedures, Brewer's, Eukaryotes, Matrix, glycans, baker's yeast, Gene, hsp70(87A), dTAFII250, hsp70Ab, HEL-S-5a, hsp70Aa, Mats, disfunctional, broad, Matrices, EfW1, presence, froggy, LC-MS-MS, Gyltl1a, Hsp70-1, polypeptide chain, dmTAF1, Taf230, Cell-to-Cell Interactions, Metabolism, Studies, hsp70Ba, hsp70Bc, hsp70Bb, Separated, Sprains, Metabolism Phenomena, Hsp-c4, dhsc70, TAF250, Taf200, matrisome, E330, Taf1p, Xhh, hsp70 87A, hsp70 87C, BPFD#36, signalling pathway, Candida robusta, grupos, time of survival, Gup1, great, ECM, has or lacks parts of type, anon-WO0118547.237, Gup2, signal transduction by cis-phosphorylation, liquid, MYOZAP, TAF, FEZ, Controlled, nutrient, anatomical structure organization, Controlling, data, TAF[[II]]250, Saccharomyces cerevisiae 'var. diastaticus', protein complex, Sprain, total expressed protein, l(3)84Ab, Eukaryotas, enzyme activity, Citronensaeure, group, strain, Metabolic Phenomena, mereological quality, HSC-70, Metabolism Concepts, count in organism, LC/MS/MS, survival, hsp68, natural protein, p230, Protein, Phenomena, hsp70, TFIID, techniques, intercellular space, Spaces, Hsp70(87C), CG18743, GCOM1, Hsp70.1, VSD1, Separation, Uralyt U, 10^[-9], TAF[[II]]230, distinct, biodegradation, Metabolic, 3-Carboxy-3-hydroxypentane-1, E(nd)195, Rest, TAF[II]250, introduction, Protein Gene Products, Eukaryotae, single-organism metabolic process, DmelCG17603, DEL, Data, hsp 70, cardinality, MMRN, groupe, Baker Yeast, proteinaceous extracellular matrix, methodology, TAF1, presence or absence in organismSaccharomyces oviformis, Yeast, matrisome, Saccharomyces cerevisiae 'var. diastaticus', Brewer's, total expressed protein, Matrix, baker's yeast, Baker, proteinaceous extracellular matrix., Saccharomyces uvarum var. melibiosus, Matrices, Saccharomyces italicus, S. cerevisiae, Baker's Yeasts, S cerevisiae, Candida robusta, Saccharomyces capensis, Saccharomyces diastaticus, Extracellular, Baker's, Mycoderma cerevisiae, Baker's Yeast, brewer's yeast, Extracellular Matrices, Proteomes, Brewer's Yeast, Baker Yeast410trueProteome of Saccharomyces cerevisiae extracellular matrixProteomic analysis of the extracellular matrix of Saccharomyces cerevisiae W303-1A Wt and the isogenic mutant strain gup1Δ during the development of multicellular 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